Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway. ‌ Lin Xie (136792)

Խորագրեր:

Validation of the intermediary proteins between β-catenin and SMAD4. ‌ Lin Xie (136792)

Խորագրեր:

Immunofluorescence analysis of the indicated proteins expressed in HeLa cells. ‌ Lin Xie (136792)

Խորագրեր:

Proximity labeling of the intermediary protein LATS1 between MOB1A and YAP1 by STUPPIT. ‌ Lin Xie (136792)

Խորագրեր:

Proximity labeling of the intermediary protein α-catenin between actin and β-catenin by STUPPIT. ‌ Lin Xie (136792)

Խորագրեր:

Validation of the YAP1 and actin related plasmids expression in the approach II of STUPPIT. ‌ Lin Xie (136792)

Խորագրեր:

Validation of the interaction of RNF20 with β-catenin and SMAD4 using PUP-IT system. ‌ Lin Xie (136792)

Խորագրեր:

Design of STUPPIT for labeling and capturing intermediary proteins between two non-interacting proteins. ‌ Lin Xie (136792)

Խորագրեր:

Proximity labeling of the intermediary protein AMOT between β-actin and YAP1 by STUPPIT. ‌ Lin Xie (136792)

Խորագրեր:

Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT. ‌ Lin Xie (136792)

Խորագրեր:

Validating the intermediary protein labeling efficiency of STUPPIT by mass spectrometry analysis. ‌ Lin Xie (136792)

Խորագրեր:

Analysis of TRIM33 interactions with β-catenin and SMAD proteins. ‌ Lin Xie (136792)

Խորագրեր: