Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway. od Lin Xie (136792)

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Validation of the intermediary proteins between β-catenin and SMAD4. od Lin Xie (136792)

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Immunofluorescence analysis of the indicated proteins expressed in HeLa cells. od Lin Xie (136792)

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Proximity labeling of the intermediary protein LATS1 between MOB1A and YAP1 by STUPPIT. od Lin Xie (136792)

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Proximity labeling of the intermediary protein α-catenin between actin and β-catenin by STUPPIT. od Lin Xie (136792)

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Validation of the YAP1 and actin related plasmids expression in the approach II of STUPPIT. od Lin Xie (136792)

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Validation of the interaction of RNF20 with β-catenin and SMAD4 using PUP-IT system. od Lin Xie (136792)

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Design of STUPPIT for labeling and capturing intermediary proteins between two non-interacting proteins. od Lin Xie (136792)

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Proximity labeling of the intermediary protein AMOT between β-actin and YAP1 by STUPPIT. od Lin Xie (136792)

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Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT. od Lin Xie (136792)

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Validating the intermediary protein labeling efficiency of STUPPIT by mass spectrometry analysis. od Lin Xie (136792)

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Analysis of TRIM33 interactions with β-catenin and SMAD proteins. od Lin Xie (136792)

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