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Suggested set of research activities and tools for the RITEC (Children's Digital Play and Well-being) research project
Veröffentlicht 2025Schlagworte: -
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Preparing materials: using a Luopan tape measure to cut a metal sheet according to phong thủy (feng shui) guidance
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Application of STUPPIT to identify novel intermediary proteins between SMAD4 and β-catenin, central hubs of BMP and Wnt pathway.
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Validation of the intermediary proteins between β-catenin and SMAD4.
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Immunofluorescence analysis of the indicated proteins expressed in HeLa cells.
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Proximity labeling of the intermediary protein LATS1 between MOB1A and YAP1 by STUPPIT.
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Proximity labeling of the intermediary protein α-catenin between actin and β-catenin by STUPPIT.
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Validation of the YAP1 and actin related plasmids expression in the approach II of STUPPIT.
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Validation of the interaction of RNF20 with β-catenin and SMAD4 using PUP-IT system.
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Design of STUPPIT for labeling and capturing intermediary proteins between two non-interacting proteins.
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Proximity labeling of the intermediary protein AMOT between β-actin and YAP1 by STUPPIT.
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Immunoblotting confirmation that β-actin-PafA-Myc ligates 3 × Flag-TbN-PupE substrate to HA-AMOT.
Veröffentlicht 2025Schlagworte: