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    Development of a CRISPR/Cas-Based Detection Platform for Tracking Decreased Susceptibility to Cephalosporins in Neisseria gonorrheae by Ziyuan Zhao (2889005)

    Published 2025
    “…We have developed the Multiplex Integrated RPA-CRISPR/Cas12a detection Assay (MIRCA) for simultaneous detection of Neisseria gonorrheae (<i>Ng</i>) and mutations with decreased susceptibility to cephalosporins. MIRCA enables multiplex detection of <i>Ng</i> and single-nucleotide polymorphisms in resistance-associated genes within 40 min, with high specificity and sensitivity (10–20 copies/reaction). …”
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