Effect of <i>abiF</i>_<i>Cd</i> overexpression on <i>C. difficile</i> and <i>E. coli.</i> gan Marion Saunier (22086633)

Pynciau:

Identification of putative AbiF/Abi_2 systems associated with phage lysis module. gan Marion Saunier (22086633)

Pynciau:

Primers used in this study. gan Marion Saunier (22086633)

Pynciau:

Growth controls for phenotypic assay in CD156 strains carrying the different plasmid constructions. gan Marion Saunier (22086633)

Pynciau:

Impact of <i>abiF</i>_<i>Cd</i> gene overexpression on <i>C. difficile</i> growth. gan Marion Saunier (22086633)

Pynciau:

Schematic representation of the workflow for the BONCAT protocol. gan Elizabeth P. Anim (22057086)

Pynciau: “...subsequent affinity purification...”

RNA-seq results from MAPS experiment. gan Marion Saunier (22086633)

Pynciau:

Distribution and environment of AbiF/Abi_2 systems in bacteria. gan Marion Saunier (22086633)

Pynciau:

Environment of <i>abiF/abi_2</i> gene. gan Marion Saunier (22086633)

Pynciau:

A ncRNA RCd22 is associated with the hypothetical AbiF_Cd system. gan Marion Saunier (22086633)

Pynciau:

Biotinylated proteins extracted with methanol/CHCl₃/water are efficiently purified with magnetic streptavidin beads. gan Elizabeth P. Anim (22057086)

Pynciau: “...subsequent affinity purification...”

Importance of <i>abiF</i> motifs and RCd22 structure for AbiF_Cd-RCd22 interaction. gan Marion Saunier (22086633)

Pynciau:

Optimizing wash conditions to reduce non-specific binding to Streptavidin-MagBeads. gan Elizabeth P. Anim (22057086)

Pynciau: “...subsequent affinity purification...”

Expression level of <i>abiF</i>_<i>Cd</i> and RCd22 mutated or not in phenotypic assays. gan Marion Saunier (22086633)

Pynciau:

RCd22 interacts with the AbiF_Cd protein. gan Marion Saunier (22086633)

Pynciau:

Potential obstacles for the successful completion of the BONCAT protocols are listed. To optimize the workflow and the quality of results, the proposed solutions can be applied eit... gan Elizabeth P. Anim (22057086)

Pynciau: “...subsequent affinity purification...”

Distribution of <i>abiF/ab</i>i_2 gene. gan Marion Saunier (22086633)

Pynciau:

Mass spectrometry results from MAPS experiment. gan Marion Saunier (22086633)

Pynciau:

Structural alignment of AlphaFold3 model of RCd22-AbiF_Cd complex with experimental structure of PbAbiF dimer bound to ncRNA (PDB identifier: 8vz6 [38]). gan Marion Saunier (22086633)

Pynciau:

Functions enriched in the vicinity of the <i>abiF/abi_2</i> gene located in prophages. gan Marion Saunier (22086633)

Pynciau: