Data Sheet 11_Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses.pdf מאת Xiao Wang (19312)

"...The lncRNF219-3:1/miR-93-5p/Tim-4 regulatory axis was validated through RNA pull-down, RNA antisense purification, and luciferase reporter assays.</p>Results<p>Tim-4 was significantly downregulated in DVT-associated macrophages, correlating with elevated proinflammatory cytokine levels. ..."

Data Sheet 1_Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses.zip מאת Xiao Wang (19312)

"...The lncRNF219-3:1/miR-93-5p/Tim-4 regulatory axis was validated through RNA pull-down, RNA antisense purification, and luciferase reporter assays.</p>Results<p>Tim-4 was significantly downregulated in DVT-associated macrophages, correlating with elevated proinflammatory cytokine levels. ..."

Expression pattern and functional analysis of MAP3K15 during WSSV infection. מאת Xiao-Qin Ran (21957039)

"...(D) The recombinant expression and purification of the S_TKC domain of MAP3K15 in <i>Escherichia coli</i> (<i>E. coli</i>). ..."

Data Sheet 5_Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses.zip מאת Xiao Wang (19312)

"...The lncRNF219-3:1/miR-93-5p/Tim-4 regulatory axis was validated through RNA pull-down, RNA antisense purification, and luciferase reporter assays.</p>Results<p>Tim-4 was significantly downregulated in DVT-associated macrophages, correlating with elevated proinflammatory cytokine levels. ..."

Data Sheet 3_Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses.zip מאת Xiao Wang (19312)

"...The lncRNF219-3:1/miR-93-5p/Tim-4 regulatory axis was validated through RNA pull-down, RNA antisense purification, and luciferase reporter assays.</p>Results<p>Tim-4 was significantly downregulated in DVT-associated macrophages, correlating with elevated proinflammatory cytokine levels. ..."

Data Sheet 4_Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses.zip מאת Xiao Wang (19312)

"...The lncRNF219-3:1/miR-93-5p/Tim-4 regulatory axis was validated through RNA pull-down, RNA antisense purification, and luciferase reporter assays.</p>Results<p>Tim-4 was significantly downregulated in DVT-associated macrophages, correlating with elevated proinflammatory cytokine levels. ..."

Data Sheet 8_Macrophage Tim-4 protects against deep vein thrombosis by binding CK2β to suppress inflammatory responses.zip מאת Xiao Wang (19312)

"...The lncRNF219-3:1/miR-93-5p/Tim-4 regulatory axis was validated through RNA pull-down, RNA antisense purification, and luciferase reporter assays.</p>Results<p>Tim-4 was significantly downregulated in DVT-associated macrophages, correlating with elevated proinflammatory cytokine levels. ..."

Adrenal proteomics מאת Adam Edholm (22256464)

"...The samples were then transferred to 3kDa spin filters (Pall Life Sciences, Ann Arbor, MI, USA) for three steps of purification. A volume of 100 µL of 50 mM NH₄HCO₃ was added to the filter, followed by centrifugation at 14000 xg for 10 mins. ..."

Chlorite exposure induces Spy production and reversible oxidation. מאת Laurent Loiseau (263538)

"...</b> Mass spectrometry analysis of the Spy protein endogenously expressed in a wild-type strain (LL1995) and a ∆<i><i>msrP</i></i> mutant (LL1996) following induction by chlorite stress. After purification and trypsin digestion, peptides containing at least one methionine residue were analyzed to assess the level of methionine oxidation. ..."

Molecular characterization of <i>Sm</i>PK1. מאת Ke Zhou (131917)

"...</b> Solubility analysis and purification of r<i><i>Sm</i></i>PK1. M: Prestained protein marker; 1: uninduced bacterial cultures; 2: the lysate of the induced recombinant bacteria harbouring pQE-80 L-r<i><i>Sm</i></i>PK1 after ultrasonication; 3: the protein in the supernatant; 4: the protein in the precipitate; 5: r<i><i>Sm</i></i>PK1 purified by the Ni-NTA-Sefinose column. ..."

IL-17A enhances PI3K-mTOR signaling in CD8⁺ T cells. מאת Farzana Nazneen (9644837)

"...<p>(A) Eight weeks old <i>Il17ra</i>^<i>-/-</i>, and WT (C57BL/6J) mice (n = 3) were infected with 100 PFU of WNV via f.p. inoculation, and the spleens were collected on D8 p.i., followed by purification of CD8⁺ T cells. The transcription profile was analyzed by RNA sequencing and the representative data were shown as a heatmap. ..."

<i>pcnB</i> contribution in sustaining <i>Shigella</i> pINV abundance. מאת Thibault Frisch (22656320)

"...(B) pINV ratio relative to wt using gDNA from the Δ<i>pcnB</i> mutant extracted by bead beating, Qiagen DNeasy Blood & Tissue Kit or Thermo Scientific GeneJET Genomic DNA Purification Kit. pINV ratio was calculated from data from <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1013727#ppat.1013727.g003" target="_blank">Fig 3A</a> and <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1013727#ppat.1013727.s002" target="_blank">S2J-K Fig</a>. wt is set as 1. ..."

RRSV Pns10 disrupts the NlNDUFS1 and NlPHB2 interaction in <i>N. lugens.</i> מאת Lianshun Zheng (22086585)

"...MBP-NlNDUFS1, His-Pns10, and GST-NlPHB2 were individually expressed in <i><i>E. coli</i></i>. After purification, different amounts of His-Pns10 were added to the MBP-NlNDUFS1 and GST-NlPHB2 mixed samples. ..."

KSHV inducible gene expression is positively regulated in a TR-dependent manner. מאת Tomoki Inagaki (12903779)

"...</b> Virions in the supernatant four days after reactivation by sodium butyrate (1 mM) and doxycycline (1 μg/ml) were treated with DNaseI, followed by DNA purification. Results are shown as viral copies per milliliter. ..."

BRD4 regulates KSHV inducible gene expression in the TR dependent manner. מאת Tomoki Inagaki (12903779)

"...</b> Virions in the supernatant four days after r.219 iSLk cells reactivated by sodium butyrate (1 mM) and doxycycline (1 μg/ml) were treated with DNaseI, followed by DNA purification. MZ1 or DMSO were added one day before reactivation. ..."

Optimization of experimental conditions and <i>pcnB</i> effect on <i>Shigella</i> pINV abundance (Supporting data for Fig 3). מאת Thibault Frisch (22656320)

"...(K) pINV relative copies per chromosome was determined by ddPCR using <i>virF</i> primers for pINV and <i>manA</i> primers for chromosome. gDNA was extracted with the Thermo Scientific GeneJET Genomic DNA Purification Kit from <i>Shigella</i> wt or Δ<i>pcnB</i> mutant grown at 30°C or 37°C at OD600 0.7. ..."

Supplementary information files for "A robust shard inspection framework with efficient throughput and energy consumption for secure geolocation-based sharded blockchains" מאת Weiquan Ni (18627421)

"...However, additional block verifications during the inspection process can incur significant block delays and energy overheads. ..."

<b>Sustainable Water Reinforcement System for Urban Areas Through Rain Capture, Transfer Logistics, and Natural Subsurface Storage</b> / <b>Propuesta de Solución Sostenible para el... מאת Ricardo Riveros (22484566)

"...</li></ul><p dir="ltr">These reservoirs may also receive treated urban rainwater or water processed through advanced purification systems.</p><h3><b>3.4. Controlled use of natural caves and shallow aquifers</b></h3><p dir="ltr">Where geology permits, stable rock caves or shallow aquifers can be used as <b>natural water reservoirs</b>, following scientific safeguards:</p><ul><li>Hydrogeological assessment (permeability, stability, recharge)...."