Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System حسب Karim E. Shalaby (12267224)

"…Using this system, we developed a novel and rapid cell-based microplate reader assay that makes possible real-time, rapid, and high throughput quantification of CRISPR nuclease activity. Cells stably expressing this dual-fluorescent reporter construct facilitated a direct quantification of the level of the internalized and functional CRISPR-Cas9 molecules into the cells without the need of co-transfecting fluorescently labeled reporter molecules. …"

A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells حسب Robin Augustine (3976964)

"…To this end, we used a simple tissue processing technique to achieve rapid imaging and quantification of invasive cells. We were able to clearly observe the migration of GFP expressing cancer cells into various organs of chicken embryo. …"

The contemporary definition of university social responsibility with quantifiable sustainability حسب Kouatli, Issam

"…Hypothetical “Green Cloud” project was used as a mechanism to show the quantification process.…"
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Quantitative MRI iron load assessment in β-thalassemia patients beyond the liver and heart: A systematic review حسب Mustafa M. Almuqbel (6629681)

"…Approximately 86% employed T2*-weighted Multi-Echo Gradient Echo sequences, reflecting their widespread acceptance for detecting paramagnetic effects of iron. ROI-based T2* quantification was the predominant image analysis method, applied in 23 studies through mono-exponential fitting of signal decay curves. …"

ABC transporter inhibition by beauvericin partially overcomes drug resistance in <i>Leishmania tropica</i> حسب Charbel Al Khoury (13167975)

"…The efficacy of ML against all developmental stages of <i>L. tropica</i> in the presence or absence of BEA was evaluated using an absolute quantification assay. The expression of resistance genes was evaluated, comparing susceptible and resistant strains. …"

Accre 8 emerging point of care CLIA system for vitamin B12 assessment compared with three established assays حسب Farah M. Trad (22466704)

"…<p dir="ltr">Accurate Vitamin B12 (Vit B12) quantification is essential for diagnosing deficiencies linked to neurological and hematological disorders. …"

Hydrogen Sulfide (H₂S) Sensor: A Concept of Physical Versus Virtual Sensing حسب Ahmed Alsarraj (16876014)

"…The proposed gas quantification model has the advantages of being low cost, easy to implement, and fast operation compared with the analytical solutions. …"

Air infiltration through building envelopes حسب Abi Shdid, Caesar

"…A generalized review of infiltration that includes evaluation techniques and models, quantification, and interaction with other heat transfer phenomena is presented in this article.…"
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The protective effects of conditioned media from human mesenchymal stem cells against Methylglyoxal-mediated injury of cultured endothelial cells حسب Maram Hasan (6672440)

"…Then, oxidative stress was quantified by DCF-DA assay and cell viability and apoptosis quantification via Annexin V & caspase 3/7 activity were assessed using flowcytometry. …"

Entitlement to Compensation for Prolongation and Disruption Costs in Construction Projects Under Standard Forms of Contract and UAE Civil Law حسب KEILANI, MOHAMMAD BASEM NAIM ZAID

"…The study identifies substantial gaps and challenges within both frameworks, including the absence of defined methodologies for assessing extensions of time, concurrent delay, and disruption quantification. Adopting a doctrinal legal analysis supported by case law, the research introduces the Society of Construction Law Delay and Disruption Protocol (2nd Edition, 2017) as a non-binding guidance document that can supplement contractual and legal procedures. …"
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Efficient automatic watershed delineation tool. (c1999) حسب Mroueh, Ahmad M.

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Long non-coding RNA MALAT1 promotes steatosis in HepG2 cells by modulating target gene expression in presence of Exendin-4 حسب Olfa Khalifa (10914452)

"…Following the starvation, a 16h incubation in DMEM containing increasing concentrations of OA (0–500 µM) at 37 °C was performed, and steatosis was quantified by triglycerides quantification, Bodipy staining, and quantification of the expression of perilipin genes family. …"

Drug Selective Poly (Vinyl chloride)-based Sensor of Desipramine Hydrochloride حسب Gupta, V.K.

"…The novel ion-pair ([TPB]- [DH]+) of the quaternary ammonium drug desipramine hydrochloride, 3-(5,6 dihydrobenzo[b][1]benzazepin-11-yl)-N-methylpropan-1-amine, has been synthesized and incorporated into a poly(vinyl chloride)-based membrane sensor for the quantification of desipramine hydrochloride in different pharmaceutical preparations. …"
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Estimation of road network reliability on resiliency حسب Soltani-Sobh, Ali

"…This study focuses on the quantification of improved reliability, which reduces the time of annealing and recovery post uncertain disruption. …"
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Evaluating antibiotic resistance gene correlations with antibiotic exposure conditions in anaerobic membrane bioreactors حسب Zarei-Baygi, Ali

"…The purpose of this study was to determine the effect of select influent antibiotics at varying concentrations on the presence and abundance of ARGs in an AnMBR system and its effluent. Quantification of targeted ARGs revealed distinct profiles in biomass and effluent, with genes conferring resistance to different antibiotic classes dominating in biomass (macrolides) and effluent (sulfonamides). …"
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A rapid, quantitative, non-radioactive bisulfite-SNuPE- IP RP HPLC assay for methylation analysis at specific CpG sites حسب El Maarri, Osman

"…The precise mapping and quantification of DNA methylation as an epigenetic parameter during development and in diseased tissues is of great importance for functional genomics. …"
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When geoscience meets generative AI and large language models: Foundations, trends, and future challenges حسب Hadid, Abdenour

"…Beyond that, GAI models show promises to the geoscience community, especially with the support to climate change, urban science, atmospheric science, marine science, and planetary science through their extraordinary ability to data‐driven modelling and uncertainty quantification.…"
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Development and applications of quaternary ammonium (QA) membrane electrodes in pharmaceutical preparation and in bioavailability of Prostaglandin E1 and Deoxycholate حسب K. Gupta, Vinod

"…The two novel ion-pairs (PB-TPB and NB-TPB) of quaternary ammonium drugs; propantheline bromide (PB), N,N-Diisopropyl-N-methyl-N-[2-(xanthen-9ylcarbonyloxy)ethyl] ammonium bromide and neostigmine bromide (NB), 3-(dimethylcarbamoyloxy) phenyl]-trimethylazanium have been synthesized, respectively and incorporated in poly (vinyl chloride)-based membrane electrodes for the quantification of propantheline bromide and neostigmine bromide in different pharmaceutical preparations. …"
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Probabilistic AutoRegressive Neural Networks for Accurate Long-Range Forecasting حسب Panja, Madhurima

"…Notably, the PARNN model provides uncertainty quantification through prediction intervals and conformal predictions setting it apart from advanced deep learning tools. …"
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Accurate and rapid prenatal diagnosis of the most frequent East Mediterranean β‐thalassemia mutations حسب Naja, R.P.

"…The technique used is based on Real‐Time PCR quantification and melting curve analysis of the amplified fragment using the LightCycler. …"
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