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    Figure 5 from Ammonia Suppresses the Antitumor Activity of Natural Killer Cells and T Cells by Decreasing Mature Perforin by Joanna Domagala (21643925)

    Published 2025
    “…NK cells were primed with IL2 (200 U/mL) and IL15 (10 ng/mL) for 24 hours before the experiment. <b>G,</b> The level of perforin detected in NK cells incubated with NH<sub>4</sub>Cl and other lysosomotropic agents [chloroquine (CQ), concanamycin (CMA), and monensin] for 4 hours determined by intracellular staining using antibodies detecting total perforin (B-D48 clone) and lysosomal perforin (δG9 clone; <i>n</i> = 2). …”
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