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BRET-based biosensors for SARS-CoV-2 oligonucleotide detection
منشور في 2024"…Specifically, we designed BioODs against the SARS-CoV-2 parental (Wuhan strain) and B.1.617.2 Delta variant through the conjugation of specific, fluorescently modified molecular beacons (sensor module) through a complementary oligonucleotide handle DNA functionalized with the NanoLuc (NLuc) luciferase protein such that the dissolution of the molecular beacon loop upon the binding of the viral oligonucleotide will result in a decrease in BRET efficiency and, thus, a change in the bioluminescence spectra. …"
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A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor
منشور في 2022"…Further, in vitro assays with the BRET-based Mpro sensors revealed a molecular crowding-mediated increase in the rate of M<sup>pro</sup> activity and a decrease in the inhibitory potential of GC376. …"
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A BRET-based Mpro biosensor containing a nanobody and tandem cleavage sites shows an increased cleavage rate
منشور في 2025"…Here, we report the engineering of a Bioluminescence Resonance Energy Transfer (BRET)-based SARS-CoV-2 main protease (Mpro) biosensor containing the Mpro N-terminal autocleavage sequence in tandem and a nanobody that shows an enhanced rate of Mpro-mediated proteolytic cleavage. …"
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Multiple-pseudogap phases in the hydrogen-doped LaFeAsO system
منشور في 2017"…The low-energy electronic structure of LaFeAsO1−H (0.0≤≤0.60), a system which exhibits two superconducting domes and antiferromagnetic orders in its phase diagram, is investigated by utilizing laser photoemission spectroscopy. …"
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LaScaDa: A Novel Scalable Topology for Data Center Network
منشور في 2020"…LaScaDa forwards packets between nodes using a new hierarchical row-based routing algorithm. …"
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Photoemission study of the electronic structure of LaFeAsO 1-xFx and LaFePO1-xFx
منشور في 2008"…We have performed a photoemission spectroscopy study of the newly discovered iron-based superconductors LaFeAsO1-xFx and LaFePO1-xFx. …"
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Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain
منشور في 2018"…Also at this locus, there was a significant increase in aggregated protein as detected by high-levels Aβ1-42. …"
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Notch Signaling Inhibition by LY411575 Attenuates Osteoblast Differentiation and Decreased Ectopic Bone Formation Capacity of Human Skeletal (Mesenchymal) Stem Cells
منشور في 2019"…Hematoxylin and eosin staining and Sirius red staining were performed to identify bone formation in vivo. Results. Among the tested molecules, LY411575, a potent γ-secretase and Notch signaling inhibitor, exhibited significant inhibitory effects on osteoblastic differentiation of hBMSCs manifested by reduced ALP activity, mineralized matrix formation, and decreased osteoblast-specific gene expression as well as in vivo ectopic bone formation. …"
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Transferrin‑modifed liposomes triggered with ultrasound to treat HeLa cells
منشور في 2021"…Calcein uptake by HeLa cells was found to be significantly higher with the Tf-PEG liposomes compared to the non-targeted control liposomes. …"
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High-sensitive detection and quantitation of thyroid-stimulating hormone (TSH) from capillary/fingerstick and venepuncture whole-blood using fluorescence-based rapid lateral flow i...
منشور في 2023"…BackgroundIn the last decade, point of care testing (POCT) such as lateral flow immunoassays (LFIA) were developed for rapid TSH measurement. …"
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Temperature dependence of the chemical potential in La2-xSrxCuO4
منشور في 2008"…We have performed x-ray photoemission spectroscopy (XPS) measurements on the high-Tc superconductor La2-xSrxCuO4 (LSCO) at various temperatures. From the shifts of core-level spectra, we have deduced the temperature dependence of the chemical potential in this system. …"
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Effect of the stretch rate during uniaxial testing on the mechanical properties of prolene
منشور في 2017"…Of the mechanical properties of special significance are the following three properties: peak load (N), extension (%) at peak load, and linear stiffness (N/mm). Uniaxial tensile testing was performed on mesh samples on a universal testing machine and involved loading different samples at 5 cross-head speeds of: 1, 10, 50, 100, and 500 mm/min. …"
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Underrepresentation of racial and ethnic minorities in cascade testing for hereditary cancer syndromes
منشور في 2023"…Cascade testing is critical as relatives found to carry a pathogenic gene variant have the opportunity to pursue targeted cancer risk reduction and early detection, decreasing cancer-associated morbidity and mortality. …"
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Adopting Geophysical Testing Techniques in Geotechnical Investigation and Shallow Foundation Design
منشور في 2018احصل على النص الكامل
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Brain microstructural changes support cognitive deficits in HIV uninfected children born to HIV infected mothers
منشور في 2020"…Significantly changed FA and MD values in different brain regions in HIC and HUC compared to CHNM were used for correlation analysis with NP test scores using Pearson’s correlation. ResultsHIC and HUC groups showed significantly decreased NP test scores in various domain compared to CHNM. …"
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Fracture Behavior of ASTM A633 steel plate specimens with edge crack at tensile test
منشور في 2017"…This paper evaluates the effect of crack length on the load-displacement curve and also fracture behavior of steel specimens is investigated after tensile tests. Results show that with crack length increasing, yield strength, the ultimate tensile strength, and fracture stress show a decreasing trend. …"
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