Effect of NRP-2 on β-catenin expression and function. by Shaija Samuel (341436)

“…Luciferase activities were measured after transient transfection of the reporter plasmids into shcntr and shNRP-2 CNDT 2.5 cells (B) Increased proteasome-mediated degradation of β-catenin in shNRP-2 cells. shcntr and shNRP-2 CNDT 2.5 cells were treated with 30 µM MG132 or equal volume of DMSO (a solvent for MG132) for 2 hours. …”

Crosstalk of daf-11/pkg-1/pde signaling in <i>C. elegans</i> analyzed by qRT-PCR and HPLC-MS/MS analysis of cGMP. by Ulrike Beckert (450918)

“…<i>Vice versa</i>, pde- deletion mutants showed reduced <i>pde-1</i> and <i>pde-5</i> gene expression connected with decreased daf-11 expression levels pointing to a direct dependency of <i>daf-11</i> and <i>pde-1</i> and <i>pde-5</i> gene expression in both directions. …”

ECOTOXICOLOGICAL ASSESSMENT OF COAL MINING WASTE by Luís Carlos Iuñes de Oliveira Filho (4630246)

“…In survival and reproduction testing of enchytraeids, the value of LC50 (estimated concentration that can cause negative effects on the survival and germination of 50 % of a group of organisms) was below 4 %, and EC50 (negative effects on reproduction) was less than 2.5 % for both soils. …”

Methionine + cystine levels for piglets from 6 to 16 kg kept under different degrees of immune system activation by Roniê Wellerson PINHEIRO (6140771)

“…It was used 360 piglets, 180 males and 180 females, weaned at 20.3 days of age and initial weight of 5.43 ± 1.17kg. Was used the experimental design of randomized blocks in a 5x2 factorial design, with five levels of total methionine + cystine (0.70; 0.77; 0.85; 0.93 e 1.01%) and two forms of immune system activation (vaccinated and unvaccinated), with four replications and nine animals per experimental unit. …”

Electrophoretic mobility shift assays with promoter deletions of <i>por</i>. by Lara Rajeev (3476492)

“…Five biotin-labeled DNA promoter substrates (I to V) of decreasing lengths (426, 348, 283, 220 and 159 bp) were used in EMSAs with purified His-tagged protein. …”

Daily qRT-PCR analysis in aggregate differentiation of hESCs. by Hananeh Fonoudi (278175)

“…<i>Isl1</i> expression was remained at high level for the next 3 days and by decreasing its expression, <i>Mef2c</i>, a cardiac progenitor marker showed its maximum expression and after that, other cardiac progenitor genes, <i>Gata4 Nkx2.5</i> and <i>Tbx5</i> reached to their highest expression level respectively. …”

Enhanced Anthropogenic Contributions to Marine Aerosols and trace elements Compared to Coastal Regions in Northern China by Yang Zhou (17600586)

“…Through positive matrix factorization (PMF) analysis, it is identified that dust and secondary nitrate contributions to PM_2.5 decreased dramatically from an average of 48.3% in coastal to 19.7% in marine environment. …”

Illustration of top two pathways and related genes restored by nicotine in the prefrontal cortex (PFC) of HIV-1Tg rats. by Junran Cao (254721)

“…APC, adenomatous polyposis coli; AXIN1, Axin-1; EPHB1, Eph receptor B1; Frizzled, frizzled receptor; GNAO1, guanine nucleotide-binding protein G(o) subunit alpha; GPCR, G protein-coupled receptor; GSK, glycogen synthase kinase; JNK, c-Jun N-terminal kinase, LRP5/6, low density lipoprotein receptor-related protein; MAPK8IP2, mitogen-activated protein kinase 8-interacting protein 2; RAC1, ras-related C3 botulinum toxin substrate 1; RHO, rhodopsin; SRC, v-src sarcoma; TCF, transcription factor; WNT5A, wingless-related MMTV integration site 5A; WNT7A, wingless-type MMTV integration site 7A.…”

Mapping of accessible sites in the target mglB mRNA by RNase T1 and rEGS libraries. by Gaoping Xiao (210986)

“…<p>A. Polyacrylamide gel electrophoresis of cleavage products of 5′end labeled mglB mRNA generated by RNase P holoenzyme and rEGSe or rEGSx libraries. …”

Dynamic changes in TF/SMs during lung maturation. by Yan Xu (14594)

“…<p>(A) mRNAs encoding TF/SMs that increased from E17.5 were functionally enriched in regulation of angiogenesis. …”

Survival plot and comparison of neoplasm distribution between DR4 and F1 mice. by Silke H. Raffegerst (255365)

“…Results were subjected to statistical analysis using GraphPad Prism v5.0 software (GraphPad Software, San Diego California USA, <a href="http://www.graphpad.com" target="_blank">www.graphpad.com</a>). …”

Actual evapotranspiration and response factors of the cowpea in Amazonian edaphoclimatic conditions by Vandeilson B. Moura (12819832)

“…The flowering stage had the greatest water demand (5.88 mm d-1). There was a significant difference in the crop yield among all treatments, with decreases of 18.91, 33.12 and 57.17% for T2, T3 and T4 in the grain yield, with a Ky of 1.48, and Kss of 2.03, 1.08, and 0.87 for the flowering, grain filling, and physiological maturation stages, respectively.…”

Table_1_Transcriptome profiling reveals the underlying mechanism of grape post-harvest pathogen Penicillium olsonii against the metabolites of Bacillus velezensis.docx by Tingfu Zhang (6700208)

“…</p>Methods<p>To investigate the mechanism of interaction between B. velezensis and P. olsonii, a draft genome was generated for P. olsonii WHG5 using the Illumina NovaSeq platform, and the transcriptomic changes in WHG5 were analyzed in response to the exposure to B. velezensis metabolites (10% v/v).…”

Image_2_Transcriptome profiling reveals the underlying mechanism of grape post-harvest pathogen Penicillium olsonii against the metabolites of Bacillus velezensis.TIFF by Tingfu Zhang (6700208)

“…</p>Methods<p>To investigate the mechanism of interaction between B. velezensis and P. olsonii, a draft genome was generated for P. olsonii WHG5 using the Illumina NovaSeq platform, and the transcriptomic changes in WHG5 were analyzed in response to the exposure to B. velezensis metabolites (10% v/v).…”

Image_1_Transcriptome profiling reveals the underlying mechanism of grape post-harvest pathogen Penicillium olsonii against the metabolites of Bacillus velezensis.TIFF by Tingfu Zhang (6700208)

“…</p>Methods<p>To investigate the mechanism of interaction between B. velezensis and P. olsonii, a draft genome was generated for P. olsonii WHG5 using the Illumina NovaSeq platform, and the transcriptomic changes in WHG5 were analyzed in response to the exposure to B. velezensis metabolites (10% v/v).…”

NFR1 and SYMRK kinase domains are able to phosphorylate LjSYMREM1 <i>in vitro</i>. by Katalin Tóth (173523)

“…Both CDs were able to phosphorylate LjSYMREM1 even though NFR1 to a lower extent than SYMRK (A). Autophosphorylation of NFR1 and SYMRK kinase domains as well as trans-phosphorylation of NFR5-CD (inactive) by NFR1 were observed. …”

Predicting Allosteric Effects from Orthosteric Binding in Hsp90-Ligand Interactions: Implications for Fragment-Based Drug Design - Fig 4 by Arun Chandramohan (2813848)

“…The absolute difference in numbers of deuterons (y-axis) between the free and ligand bound state is plotted for each pepsin digest fragment listed from the N to C terminus (x-axis) of Hsp90 for each deuterium exchange time point (t = 0.5, 2, 5, 10 min) in a ‘difference plot’. Shifts in the positive scale represent decreases in deuterium exchange and shifts in the negative scale represent increases in deuterium exchange when compared to apo form of Hsp90. …”

Knockdown of <i>cpa6</i> reduces pilocarpine-induced increase in locomotion in 3 dpf zebrafish embryos. by Mark William Lopes (2601118)

“…The number of crossings was recorded over a 1.5 minute period by an observer blinded to the treatment group; for baseline the period from 8.5–10.0 min was analyzed, and for pilocarpine the period from 5.0–6.5 min after drug treatment was analyzed. …”

Data_Sheet_4_Bamboo charcoal mediated plant secondary metabolites biosynthesis in tomato against South American tomato pinworm (Tuta absoluta).xlsx by Limin Chen (90676)

“…Studies have shown that biochar (BC) amendment to soil can enhance plant growth and resistance to herbivory.</p>Methods<p>First, we quantified the morphological performance of tomato plants grown in different coconut bran and bamboo charcoal (v/v) combinations and selected the most beneficial one. …”

Data_Sheet_2_Bamboo charcoal mediated plant secondary metabolites biosynthesis in tomato against South American tomato pinworm (Tuta absoluta).xlsx by Limin Chen (90676)

“…Studies have shown that biochar (BC) amendment to soil can enhance plant growth and resistance to herbivory.</p>Methods<p>First, we quantified the morphological performance of tomato plants grown in different coconut bran and bamboo charcoal (v/v) combinations and selected the most beneficial one. …”