Knocking down and overexpression of EBI3 by siRNA and plasmid DNA have a constant effect during chondrogenesis of human MSCs. by Tong Zhang (103827)

microRNA-34a-Mediated Down-Regulation of the Microglial-Enriched Triggering Receptor and Phagocytosis-Sensor TREM2 in Age-Related Macular Degeneration - Fig 6 by Surjyadipta Bhattacharjee (3181674)

“…After vacuum evaporation of HFIP, aliquoted peptide was re-suspended with DMSO to 5 mM and diluted to 5 μM into the cell culture media; <b>(B)</b> cells were treated with a scrambled miRNA-34a sequence (miRNA-34a-<b>sc</b>; 30 nM;, control, <b>a-d</b>, middle row); or <b>(C)</b> with an LNA-stabilized miRNA-34a (30 nM; miRNA-34a stressed; <b>a-d</b>; bottom row); treatments were for 24 hr before incubation with 5 uM of Aβ42 (made up as in [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref033" target="_blank">33</a>]) for another 24 hr before assay; MG cells were subsequently stained using a murine amyloid beta MABN10 (red fluorescence λ_max~650 nm; anti-Aβ antibody, clone W0-2; Millipore, Bellerica MA), a TREM-2 antibody (M-227): sc-48765 (green fluorescence; λ_max~510 nm; Santa Cruz, Santa Cruz CA) or DAPI nuclear stain (blue fluorescence; λ_max~470 nm) as in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a>;</b> arrows indicate Aβ42 uptake into MG cells; note decreased presence of TREM2 in miRNA-34a treated MG cells (bottom row, panel B) and decrease in ingested Aβ42 peptide within C8B4 cells (<b>C</b>; bottom row, panel <b>d</b>). …”

miR-302d reverts TGFB-induced EMT in hRPE cells. by Xiaonan Hu (1235328)

Subjects:

Pollution Characteristics of Particulate Matter (PM2.5 and PM10) and Constituent Carbonaceous Aerosols in a South Asian Future Megacity by Aslam, Afifa

Get full text
Get full text

Controlling Electron/Hole Recombination in Near-Infrared Polymer Phototransistors through an Insulation Medium: A Pathway to Ultrahigh Photosensitivity by Tao Han (22342)

“…The Δ<i>V</i>_th value (∼29.5 ± 1.0 V) increased by approximately 4-fold, the <i>I</i>_ph/<i>I</i>_dark (∼4.4 × 10⁶) increased by a factor of 3000 to 4000-fold, and the dark current decreased by 2–3 orders of magnitude (@ <i>V</i>_g = 0 V). …”

Single-Molecule Magnets:  Structural Characterization, Magnetic Properties, and ¹⁹F NMR Spectroscopy of a Mn₁₂ Family Spanning Three Oxidation Levels by Nicole E. Chakov (2638930)

“…DC studies on dried microcrystalline samples of <b>2</b>, <b>3</b>, and <b>4</b>·2.5C₇H₈ restrained in eicosane in the 1.80−10.0 K and 1−70 kG ranges were fit to give <i>S</i> = 10, <i>D</i> = −0.40 cm^-1, <i>g</i> = 1.87, |<i>D</i>|/<i>g</i> = 0.21 cm^-1 for <b>2</b>, <i>S</i> = 19/2, <i>D</i> = −0.34 cm^-1, <i>g</i> = 2.04, |<i>D</i>|/<i>g</i> = 0.17 cm^-1 for <b>3</b>, and <i>S</i> = 10, <i>D</i> = −0.29 cm^-1, <i>g</i> = 2.05, |<i>D</i>|/<i>g</i> = 0.14 cm^-1 for <b>4</b>, where <i>D</i> is the axial zero-field splitting parameter. …”

Iridium(III, 0, and −I) Complexes Stabilized by 1,1‘-Bis(diphenylphosphino)ferrocene (dppf):  Synthesis and Characterization. Crystal Structures of [Na(THF)₅][Ir(dppf)<s... by Bruno Longato (2315767)

“…<b>3</b> crystallizes in the triclinic system, space group <i>P</i>1̄, with <i>a</i> = 13.019(4) Å, <i>b</i> = 13.765(6) Å, <i>c</i> = 15.549(5) Å, α = 93.74(3)°, β = 90.35(3)°, γ = 92.07(3)°, <i>V</i> = 2779(2) ų, and <i>Z</i> = 2. …”

Gossypol does not alter inflammatory cell profiles in the lung. by Jennifer L. Judge (5278415)

Phosphorylation of p66^Shc at Ser36 during reperfusion after ischemia was dependent on the duration of ischemia. by Meiying Yang (654532)

NINL and DZANK1 associate specifically with complementary subunits of the cytoplasmic dynein 1 motor complex. by Margo Dona (816015)

“…(h) Quantification of photoreceptor outer segment length revealed a significantly decreased length of outer segments in <i>dync1h1</i> (3ng/nl) and <i>dync1h1/ninl</i> double morphants as compared to controls (*** <i>P</i><0.0001; two-tailed, unpaired Student’s <i>t</i>-test). …”

A feeding protocol for delivery of agents to assess development in Varroa mites by Ana R. Cabrera (3966545)

“…Although supplementation with <i>Bombyx mori</i> Bm5 cells increased the amount of hemolymph consumed per mite, there was a significant decrease in the percentage of mites that fed and survived. …”

BuGZ binds to the <i>cat-3</i> gene region specifically and its recruitment responses to <i>cat-3</i> overexpression caused by oxidative stress. by Yike Zhou (12800411)

Influence of T180A-SHIP2 on LEC functional responses. by Germaine D. Agollah (623510)

“…Data are presented as % of area covered by migrated cells on underside of transwell filters and cells stained with DRAQ5. (<b>D</b>) WT-SHIP2 exhibits increased adhesion (assessed by crystal violet staining) over Vector on BSA and collagen while T180A-SHIP2 shows decreased adhesion to collagen compared to WT. …”

The transcription levels of <i>NbSGS3-CFP-HA</i> (A) and <i>YFP-NbSGS3</i> (B) in Fig 1F and Fig 1G, respectively. by Yongle Zhang (11355546)

Expression of ECM markers on day 5 of post infection. by Sobia Manzoor (11372369)

Expression of various cytokines on day 5 of post infection. by Sobia Manzoor (11372369)

The characteristics of patient groups were used to prove the method’s applicability. by Ewa Paszkowska (21246702)

Subjects:

The mevalonate pathway (cholesterol biosynthesis): the place of action of statins, and their main effects. by Ewa Paszkowska (21246702)

Subjects:

The RSD results were obtained for different proportions of the extraction solvent/tissue (each proportion was prepared in triplicate repetition, n = 3). by Ewa Paszkowska (21246702)

Subjects:

Gossypol is an effective anti-fibrotic treatment. by Jennifer L. Judge (5278415)