Supplementary Figures from AZD9496: An Oral Estrogen Receptor Inhibitor That Blocks the Growth of ER-Positive and <i>ESR1</i>-Mutant Breast Tumors in Preclinical Models by Hazel M. Weir (2123344)

“…<p>Supplementary Figure 1 contains a Western blot showing the amount of estrogen receptor remaining after treatment of MCF-7 cells with AZD9496, fulvestrant and estradiol alongside GAPDH protein levels as an internal gel loading control; Supplementary Figure 2 shows the rate of decreased detection by mass spectrometry of an estrogen receptor heavy isotype labelled peptide over time after treatment of cells with AZD9496, fulvestrant and tamoxifen and the calculated half life; Supplementary Figure 3 shows the degradation of estrogen receptor α by AZD9496, fulvestrant and estradiol in the presence of +/- 10 μM proteosomal inhibitor MG132; Supplementary Figure 4 contains a Western blot showing the re-emergence over 48 hours of estrogen receptor α following removal of AZD9496, fulvestrant and estradiol compounds from MCF-7 cells; Supplementary Figure 5 shows a bar chart of the measured estradiol levels in the blood of mice over 42 days after implantation of the mice with estrogen pellets to support growth of the MCF-7 cells as a tumour in mice; Supplementary Figure 6 shows the effects of AZD9496, fulvestrant and tamoxifen on mRNA levels of estrogen receptor regulated genes in vitro and in vivo; Supplementary Figure 7 shows the levels of estrogen receptor α protein remaining in tumours from the HCC1428 LTED model after treatment with AZD9496 and fulvestrant.…”

Iron loading and response to oxidative stress of iMEFs. by Holly J. Garringer (281855)

“…<p>Compare to non-transgenic wild-type (WT) control cells, iMEFs from FTL-Tg mice show a decreased viability in the presence of increasing concentrations of iron (A). iMEFs from FTL-Tg mice accumulated a significantly higher level of intracellular iron after 3 days of iron (100 μM) loading (p < 0.001). …”

Cancer cells can produce ATP in response to extracellular acid alone. by Gautam Dhar (723875)

“…<p>(A) Time kinetics of ATP production at pH 7.0. MDA-MB-231 (steady state), at pH 7.5 in glucose-free buffer, were acidified to pH 7.0 at 37°C for the indicated time, quenched with chloroform and the amounts of ATP were determined from the aqueous fraction. …”

Cell cycle indexes of oNSCs in the developing OE show selective defects in <i>BAF155</i>cKO mutants. by Christina Bachmann (3111933)

“…(C, D) Statistical analyses showed that the loss of BAF155 led to a selective decrease in pHH3⁺ oNSCs at early stages (E10.5–E11.5) (B), Pax6⁺ oNSCs at 10.5 (C), and basal pHH3⁺ oNSCs at E13.5 (B), but not apical pHH3⁺ SUS cells (B) or pHH3⁺/Tbr2⁺ IPs (D). …”

Table_1_Cytokines, Adipokines, and Bone Markers at Rest and in Response to Plyometric Exercise in Obese vs Normal Weight Adolescent Females.docx by Nigel Kurgan (6533759)

“…CTX was similar between groups at rest and decreased (-24%; p < 0.001) 1 h post-exercise. Sclerostin was similar between groups at rest, but there was a significant interaction reflecting a significant increase (+29%; p = 0.04) 5 min post-exercise in the obese group and a non-significant decrease (-13%; p = 0.08) in normal weight controls. …”

Randomized Placebo-Controlled Phase II Trial of Autologous Mesenchymal Stem Cells in Multiple Sclerosis by Sara Llufriu (323179)

“…At 6 months, patients treated with MSCs had a trend to lower mean cumulative number of GEL (3.1, 95% CI = 1.1–8.8 vs 12.3, 95% CI = 4.4–34.5, p = 0.064), and at the end of study to reduced mean GEL (−2.8±5.9 vs 3±5.4, p = 0.075). …”

Image_3_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.TIF by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Image_2_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.TIF by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Table_2_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.XLSX by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Table 1_PBMCs gene expression predicts liver fibrosis regression after successful HCV therapy in HIV/HCV-coinfected patients.docx by Ana Virseda-Berdices (9638081)

“…</p>Methods<p>We performed a retrospective study in 48 HIV/HCV-coinfected patients who started anti-HCV treatment with at least advanced fibrosis (LSM ≥9.5). …”

Table_4_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.XLSX by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Data Sheet 1_PBMCs gene expression predicts liver fibrosis regression after successful HCV therapy in HIV/HCV-coinfected patients.docx by Ana Virseda-Berdices (9638081)

“…</p>Methods<p>We performed a retrospective study in 48 HIV/HCV-coinfected patients who started anti-HCV treatment with at least advanced fibrosis (LSM ≥9.5). …”

Image_1_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.TIF by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Table_1_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.XLSX by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Image_4_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.TIF by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

Table 2_PBMCs gene expression predicts liver fibrosis regression after successful HCV therapy in HIV/HCV-coinfected patients.docx by Ana Virseda-Berdices (9638081)

“…</p>Methods<p>We performed a retrospective study in 48 HIV/HCV-coinfected patients who started anti-HCV treatment with at least advanced fibrosis (LSM ≥9.5). …”

Lab 2:Biol2050 Woodlot Data Set 4 by Katrin Chapkis (797943)

“…The tree was considered a sapling when it just reached the observer's height (5"4), or slightly taller. …”

Table_3_Hypoxia Alters Epigenetic and N-Glycosylation Profiles of Ovarian and Breast Cancer Cell Lines in-vitro.XLSX by Gordon Greville (9182312)

“…SiRNA transient knock down of GATA2/3 transcription factors resulted in a decrease in the expression of glycosyltransferases ST3GAL4 and MGAT5, which are responsible for sialylation and branching, respectively.…”

The high tolerance to aluminium in crucian carp (<i>Carassius carassius</i>) is associated with its ability to avoid hypoxia by Antonio B. S. Poléo (4169929)

“…The decrease in plasma ions caused a transient increase in haematocrit and water content in muscle tissue, returning to control levels when the ion concentrations stabilised, suggesting that the water balance was restored. …”

Stability and feasibility: Outline. by Michaël Dougoud (3603548)

“…<p>(<b>a</b>) For a system to be feasible, interaction strength has to decrease with complexity. …”