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1861
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1862
The interaction between E1A and PKA subunits is required for full expression of HAdV-5 E3 and E4 transcripts.
Published 2016“…Results for the HAdV-5 E1A (A), E1B (B), E2 (C), E3 (D) and E4 (E) transcription units are shown. …”
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1863
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1864
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1865
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1868
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1869
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1871
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1872
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1873
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1874
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1875
Cortical distribution of neurons born at E11.5, E13.5, E15.5, or E17.5 in P0 mice.
Published 2015“…In <i>Nestin;Epha4</i><sup><i>fx/fx</i></sup> mice, a larger number of CTIP2+ cells was co-labeled with BrdU at E11.5 but fewer were co-labeled at E13.5 compared to controls (C), whereas in <i>GFAP;Epha4</i><sup><i>fx/fx</i></sup> mice, the number of co-labeled cells was similar to that of controls at both E11.5 and E13.5 (D). …”
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1876
Activated CD4+ CD45RO+ memory T-cells decrease secretion of soluble caspase-1 and P2X7R mRNA expression in monocytes leading to a reduced response upon ATP binding.
Published 2012“…A significant decrease of soluble Caspase-1 in the supernatant of cells co-cultured with activated memory T-cells was observed both in the presence and absence of IFNβ; b) intracellular levels of pro-IL-1β measured with an ELISA specific for the immature precursor of IL-1β showed a significant increase in pro-IL-1β levels in the presence of human activated CD4+CD45RO+ memory T-cells (n = 3), cell lysates from adherent monocytes were obtained after removal of non-adherent T-cells after addition of LPS and prior to incubation with ATP c) the ratio of intracellular pro-IL-1β to released soluble Caspase-1 is significantly increased in the presence of human activated CD4+CD45RO+ memory T-cells (n = 3). d) <i>P2X7R</i> mRNA expression in monocytes cultured overnight with activated CD4+CD45RO+ memory T-cells is decreased with and without addition of exogenous IFNβ (n = 3), mRNA was isolated from adherent monocytes after removal of non-adherent T-cells after addition of LPS and prior to incubation with ATP (Data are shown as means ± SD of duplicate cultures; *p<0.05; **p<0.01 employing repeated measures ANOVA with post-hoc Bonferroni adjustment for multiple comparisons to avoid random correlations); e) Ca<sup>2+</sup>-influx measurement in monocytes cultured with activated human CD4+CD45RO+ memory T-cells in the presence of IFNβ demonstrates the absence of slow sustained Ca<sup>2+</sup>-influx mediated by ATP binding to the purinergic P2X7-receptor (representative of 3 independent experiments); f) Annexin V staining of monocytes coincubated with T-cells and stimulated with LPS was measured by flow cytometry (representative flow cytometry histogram of n = 5).…”
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1877
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1878
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1880