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98001
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98002
Mitochondrial respiratory chain deficiency inhibits lysosomal hydrolysis
Published 2019“…</p> <p><b>Abbreviation</b>: ΔΨ<sub>m</sub>: mitochondrial transmembrane potential; AMP: adenosine monophosphate; AMPK: AMP-activated protein kinase; ATG5: autophagy related 5; ATP: adenosine triphosphate; ATP6V0A1: ATPase, H+ transporting, lysosomal, V0 subbunit A1; ATP6V1A: ATPase, H+ transporting, lysosomal, V0 subbunit A; BSA: bovine serum albumin; CCCP: carbonyl cyanide-m-chlorophenylhydrazone; CREB1: cAMP response element binding protein 1; CTSD: cathepsin D; CTSF: cathepsin F; DMEM: Dulbecco’s modified Eagle’s medium; DMSO: dimethyl sulfoxide; EBSS: Earl’s balanced salt solution; ER: endoplasmic reticulum; FBS: fetal bovine serum; FCCP: carbonyl cyanide-p-trifluoromethoxyphenolhydrazone; GFP: green fluorescent protein; GPN: glycyl-L-phenylalanine 2-naphthylamide; LAMP1: lysosomal associated membrane protein 1; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MCOLN1/TRPML1: mucolipin 1; MEF: mouse embryonic fibroblast; MITF: melanocyte inducing transcription factor; ML1N*2-GFP: probe used to detect PtdIns(3,5)P<sub>2</sub> based on the transmembrane domain of MCOLN1; MTORC1: mechanistic target of rapamycin kinase complex 1; NDUFS4: NADH:ubiquinone oxidoreductase subunit S4; OCR: oxygen consumption rate; PBS: phosphate-buffered saline; pcDNA: plasmid cytomegalovirus promoter DNA; PCR: polymerase chain reaction; PtdIns3P: phosphatidylinositol-3-phosphate; PtdIns(3,5)P<sub>2</sub>: phosphatidylinositol-3,5-bisphosphate; PIKFYVE: phosphoinositide kinase, FYVE-type zinc finger containing; P/S: penicillin-streptomycin; PVDF: polyvinylidene fluoride; qPCR: quantitative real time polymerase chain reaction; RFP: red fluorescent protein; RNA: ribonucleic acid; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis; shRNA: short hairpin RNA; siRNA: small interfering RNA; TFEB: transcription factor EB; TFE3: transcription factor binding to IGHM enhancer 3; TMRM: tetramethylrhodamine, methyl ester, perchlorate; ULK1: unc-51 like autophagy activating kinase 1; ULK2: unc-51 like autophagy activating kinase 2; UQCRC1: ubiquinol-cytochrome c reductase core protein 1; v-ATPase: vacuolar-type H+-translocating ATPase; WT: wild-type</p>…”
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98003
Resveratrol inhibited the increase in <i>I</i><sub>Na.L</sub> induced by 300 µM H<sub>2</sub>O<sub>2</sub>.
Published 2013“…<p><i>A</i>. 10, 20, 40 and 80 µM resveratrol decreased the amplitudes of <i>I</i><sub>Na.L</sub> in the presence of 300 µM H<sub>2</sub>O<sub>2</sub> in a concentration dependent manner. …”
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98004
Applying tACS during physiological oscillations results in bidirectional changes in spike timing.
Published 2022“…Data were collected (C) in the presence of a 5 Hz ongoing oscillation within V4 (<i>N</i> = 157), (D) also in V4, but at 20 Hz, where the oscillation is weaker (<i>N</i> = 123), and (E) again at 5 Hz but in the hippocampus (HC; <i>N</i> = 21), which also lacks strong 5 Hz oscillations under our conditions. …”
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98005
Effects of autophagic regulation on cardiac function.
Published 2013“…Continuous K-H buffer perfusion without medication was used as a negative control. <b>A and B.</b> The left ventricular systolic pressure (LVSP) and the maximal rate of the rise/drop of left ventricular pressure (± dp/dt max) decreased in the hearts perfused with the autophagy activator rapamycin. …”
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98006
MMR gene expression in cancer and benign hyperplasia of prostate glands.
Published 2015“…Fold changes and statistical significance are indicated. * indicates P <0.05 and n indicates the number of samples. (e) Photomicrograph of representative prostate archival specimens immune-stained with antibodies against hMLH1, hMSH6 and hMSH2 at 400x magnification showing decreased expression of the MMR genes in prostate cancer tissues compared to benign hyperplastic tissues.…”
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98007
Distinguishing orthosteric and allosteric effects in Hsp90.
Published 2016“…<p><b>(A)</b> The absolute difference in numbers of deuterons (y-axis) between the free and ligand bound state is plotted for each pepsin digest fragment listed from the N to C terminus (x-axis) of Hsp90 for each deuterium exchange time point (t = 0.5, 2, 5, 10 min) in a ‘difference plot’. …”
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98008
Responses to interactions between different concentrations of cannabidiol (CBD) and WIN55,212–2 (WIN) during drug exposure.
Published 2020“…Interactions of CBD1 + WIN0.5 (μg/mL) induced significant increases in activity during the fifth and sixth dark intervals, whereas CBD10 + WIN0.5 (μg/mL) induced decreases from the second to sixth light intervals. …”
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98009
Quantification of GFAP/PCNA-positive cells in APC in young and culture aged APC upon hypoxia/Glu/EPO-exposure.
Published 2013“…Hypoxia decreased the proliferation of APC (cf- N+Glu vs. H+Gu); (<b>B</b>) Quantification of GFAP/PCNA+ cells on DIV21 shows no changes in PCNA+ astrocytes exposed to EPO under normoxia (white bars), while a significant increase (**p<0.01) is detected in EPO-treated APC (H+Glu+5U E) under hypoxia (grey bars), as compared with the hypoxic control (H+Glu). …”
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98010
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98011
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98012
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98013
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98014
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98015
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98016
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98017
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98018
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98019
Table_1_Feasibility and Effect of Physiological-Based CPAP in Preterm Infants at Birth.pdf
Published 2021“…Measurements were available for analysis in 28 infants (PB-CPAP n = 8, 5–8 cmH<sub>2</sub>O n = 20). Protocol deviations in the PB-CPAP group included one infant receiving 3 inflations with 15 cmH<sub>2</sub>O PEEP and two infants in which CPAP levels were decreased faster than described in the study protocol. …”
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98020
The Interaction of LFA-1 on Mononuclear Cells and ICAM-1 on Tubular Epithelial Cells Accelerates TGF-β1-Induced Renal Epithelial-Mesenchymal Transition
Published 2011“…The expression of the active form of LFA-1 on PBMCs did not increase after co-culture with not CCL3 but CXCL12 knockdown HK-2-TGF-β<sub>1</sub> (0.1). The expression of epithelial cell junction markers (E-cadherin and occludin) further decreased and that of mesenchymal markers (vimentin and fibronectin) further increased in HK-2-TGF-β<sub>1</sub> (0.1) after co-culture with PBMCs for 24 hrs (HK-2-TGF-β<sub>1</sub> (0.1)-PBMCs). …”