Activated CD4+ CD45RO+ memory T-cells decrease secretion of soluble caspase-1 and P2X7R mRNA expression in monocytes leading to a reduced response upon ATP binding. by Vanessa Beynon (153924)

“…A significant decrease of soluble Caspase-1 in the supernatant of cells co-cultured with activated memory T-cells was observed both in the presence and absence of IFNβ; b) intracellular levels of pro-IL-1β measured with an ELISA specific for the immature precursor of IL-1β showed a significant increase in pro-IL-1β levels in the presence of human activated CD4+CD45RO+ memory T-cells (n = 3), cell lysates from adherent monocytes were obtained after removal of non-adherent T-cells after addition of LPS and prior to incubation with ATP c) the ratio of intracellular pro-IL-1β to released soluble Caspase-1 is significantly increased in the presence of human activated CD4+CD45RO+ memory T-cells (n = 3). d) <i>P2X7R</i> mRNA expression in monocytes cultured overnight with activated CD4+CD45RO+ memory T-cells is decreased with and without addition of exogenous IFNβ (n = 3), mRNA was isolated from adherent monocytes after removal of non-adherent T-cells after addition of LPS and prior to incubation with ATP (Data are shown as means ± SD of duplicate cultures; *p<0.05; **p<0.01 employing repeated measures ANOVA with post-hoc Bonferroni adjustment for multiple comparisons to avoid random correlations); e) Ca²⁺-influx measurement in monocytes cultured with activated human CD4+CD45RO+ memory T-cells in the presence of IFNβ demonstrates the absence of slow sustained Ca²⁺-influx mediated by ATP binding to the purinergic P2X7-receptor (representative of 3 independent experiments); f) Annexin V staining of monocytes coincubated with T-cells and stimulated with LPS was measured by flow cytometry (representative flow cytometry histogram of n = 5).…”

sEH inhibition decreased fasting glucose levels in type 2 diabetic mice. by Kristen L. Zuloaga (563616)

PTB is associated with decreased spontaneous production of Type 1, 2, 17 and immunoregulatory cytokines. by Nathella Pavan Kumar (129299)

Repeated dosing of P2Y13R agonist decreases HDL-C. by Marine Goffinet (90787)

IF analysis of N-cadherin in esophageal tissue from pediatric control (0–5 Eos/HPF) and EoE patients (>15 Eos/HPF). by Elizabeth Garcia (23115)

IF analysis of TWIST1 in esophageal tissue from pediatric control (0–5 Eos/HPF) and EoE patients (>15 Eos/HPF). by Elizabeth Garcia (23115)

Comparison of variable distributions between the women in SAVOR-TIMI 53 trial based dataset v.s. the virtual patient dataset. by Yasunori Aoki (339027)

Comparison of variable distributions between the women in SAVOR-TIMI 53 trial based dataset v.s. the virtual patient dataset. by Yasunori Aoki (339027)

Cardiac Development in Zebrafish and Human Embryonic Stem Cells Is Inhibited by Exposure to Tobacco Cigarettes and E-Cigarettes by Nathan J. Palpant (127912)

“…In definitive human cardiomyocytes, both e-cigarette- and tobacco cigarette-treated samples showed reduced expression of sarcomeric genes such as MLC2v and MYL6. …”

Cell proliferation in the MGE is not altered in Fgfr1 mutants at E13.5. by Karen Müller Smith (613941)

All data points from Fig 2. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 8. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 3. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 1. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 4. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 9. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 7. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

All data points from Fig 6. by Sara Hijazi (21656615)

“…Following a remyelination period of 5 weeks, PV interneuron properties were only partially recovered, suggesting that transient juvenile demyelination leads to long-lasting impairments of PV interneuron function. …”

High glucose-containing media decreases mitochondrial membrane potential and ATP levels of in mixed glia cultures. by Yung-Cheng Huang (3443450)

Subjects:

The raw data for Fig 5B. by Hongjoo An (21703199)

Subjects: