Human Engineered Heart Tissue as a Versatile Tool in Basic Research and Preclinical Toxicology by Sebastian Schaaf (200540)

“…However, high content experimental platforms are lacking that allow the function of hESC-derived cardiomyocytes to be studied under relatively physiological and standardized conditions. Here we describe a simple and robust protocol for the generation of fibrin-based human engineered heart tissue (hEHT) in a 24-well format using an unselected population of differentiated human embryonic stem cells containing 30–40% α-actinin-positive cardiac myocytes. …”

Gene expression profiles in wildtype, <i>Tnfr1</i>- and <i>Tnfr2-</i>deficient glomeruli after TNF stimulation ex vivo. by Anela Taubitz (433585)

“…The number of genes identified in <i>Tnfr1</i>−/− glomeruli was lower than in <i>Tnfr1,2</i>−/− glomeruli, but all genes differentially expressed in <i>Tnfr1</i>−/− glomeruli were regulated similarly in <i>Tnfr1,2</i>−/− glomeruli, although for some genes pair-wise comparison between <i>Tnfr1,2</i>−/− to wildtype did not reach significance or was below the pre-defined 1.5-fold cut-off (see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068167#pone.0068167.s004" target="_blank">Table S3</a>). …”

Genetic Ancestry-Smoking Interactions and Lung Function in African Americans: A Cohort Study by Melinda C. Aldrich (110234)

“…</p> <h3>Methodology/Principal Findings</h3><p>We evaluated a prospective ongoing cohort of 1,281 African Americans participating in the Health, Aging, and Body Composition (Health ABC) Study initiated in 1997. …”

Serotonin, a downstream effector of GLP-2, enhances lacteal contractility and lymph flow by Lili Tian (12992016)

“…Utilizing a rat lymph fistula model, 5 hours after an intraduodenal (i.d.) lipid bolus, the following intraperitoneal (i.p.) administrations were applied in two different sets of experiments: Experiment 1: 1) Placebo, 2) GLP-2, 3) GLP-2 + Ketanserin (serotonin receptor antagonist). …”

Effects of Varied Housing Density on a Hybrid Mouse Strain Followed for 20 Months by Beverly Paigen (18314)

“…<div><p>To evaluate the effect of increased housing density in a hybrid mouse strain, we evaluated a panel of physiological and behavioral traits in animals that were housed in groups of 3, 5, 8, or 12, using cages that provide 78.1 in² of floor space. …”

Presentation1_Exchange flow in a highly stratified fjord in drought conditions.pdf by Elias Pinilla (20400329)

“…Using a high-resolution hydrodynamic model and the Total Exchange Flow (TEF) framework, we analyzed the impacts of river discharge, tides, and wind during a dry year (2016) and a typical year (2018). …”

A Method for Selectively Enriching Microbial DNA from Contaminating Vertebrate Host DNA by George R. Feehery (477312)

“…We report enrichment of DNA samples from human saliva, human blood, a mock malaria-infected blood sample and a black molly fish. …”

Mechanism of insulin resistance in a rat model of kidney disease and the risk of developing type 2 diabetes by François Dion (3990335)

“…The effect of chronic kidney disease on insulin resistance was studied on two different rat strains. First, in a 5/6^th nephrectomised Sprague-Dawley rat model of chronic kidney disease, we observed a correlation between the severity of chronic kidney disease and hyperglycemia as evaluated by serum fructosamine levels (p<0.0001). …”

Transcriptome and targeted metabolome analyses of muscles from <i>PPP1CC</i>-SSKO and control mice. by Jiahong Sun (6497603)

Simulated Meta-Analysis. by Laurynas Kalesinskas (5768810)

“…<p>A) Using simulated data in five studies, we explored the effect of changing the effect size and variance of a single study on each model’s pooled effect size, heterogeneity (τ²) and significance. …”

The interaction between tonic GABA_AR signaling and glutamatergic activity. by Michelle Day (11617621)

“…Increasing depolarization (2–3 mV to 10⁻⁵ S/cm² density) was accompanied by a dendritic spike in response to a glutamatergic input that was subthreshold in the absence of tonic GABA activation. …”

A systematic review and meta-analysis of preclinical trials testing anti-toxin therapies for <i>B</i>. <i>anthracis </i>infection: A need for more robust study designs and results by Wanying Xu (3765427)

“…Across all agents and species, agents decreased RR [0.64(0.52,0.79); I² = 5.3%, p = 0.39].…”

2′-<em>O</em> Methylation of the Viral mRNA Cap by West Nile Virus Evades Ifit1-Dependent and -Independent Mechanisms of Host Restriction In Vivo by Kristy J. Szretter (165885)

“…Here, we examined the role of Ifit1 in restricting pathogenesis <em>in vivo</em> of wild type WNV (WNV-WT) and a mutant in the NS5 gene (WNV-E218A) lacking 2′-<em>O</em> methylation of the 5′ viral RNA cap. …”

Analysis of the care of patients with Diabetes Mellitus and Hypertension: the experience of a small municipality in Brazil by Monica Viegas Andrade (5621213)

DataSheet1_Understanding the impact of radiotherapy fractionation on overall survival in a large head and neck squamous cell carcinoma dataset: a comprehensive approach combining m... by Igor Shuryak (337356)

“…Our previous analyses of radiotherapy-only HNSCC clinical trials data using mechanistically-motivated models of tumor repopulation and killing by radiotherapy predicted that hyperfractionation with twice-daily fractions, or hypofractionation involving increased doses/fraction and reduced treatment durations, both improve tumor control and reduce late normal tissue toxicity, compared with standard protocols using 35×2 Gy. Here we further investigated the validity of these conclusions by analyzing a large modern dataset on 3,346 HNSCC radiotherapy patients from the University Health Network in Toronto, Canada, where 42.5% of patients were also treated with chemotherapy.…”

Supplementary Material for: Eppikajutsuto Protects against Food Allergy Induced by Ovalbumin in a Murine Model by Matsui T. (4061857)

Efficacy and safety of a single radiofrequency ablation of solid benign non-functioning thyroid nodules by Roberto Cesareo (4676131)

Pancreatic Differentiation of Stem Cells Reveals Pathogenesis of a Syndrome of Ketosis-Prone Diabetes by Diane Yang (11198946)

“…Genetic analysis of an adult patient with an unusual course of Ketosis-Prone Diabetes (KPD) and lacking islet autoantibodies demonstrated a nucleotide variant in the<i> </i>5’-UTR of <i>PDX1</i>, a beta-cell development gene. …”

Mubritinib and canertinib perturb CNC migration <i>ex vivo</i>. by Ketan Mathavan (4634704)

“…(V) Fold change in explant surface area over time. …”

Intrinsic and extrinsic fluorescence spectroscopy of the protease 1147. by Hossein Tarrahimofrad (9016994)