Efficacy and selectivity of the shSNP <i>in vitro</i>. by Valérie Drouet (3179907)

“…Levels of the chimeric <i>HTT</i> mRNA were normalized to β-Actin and are reported as mean percentages relative to the control condition (set at 100%) ± SEM (n = 3–5). …”

Self-limiting of the gTLR9 signaling by <i>gTlr9</i> alternative splicing. by Frank Fang-Yao Lee (2210863)

“…DRB pre-treatment (0 h) led to slight increase of <i>9A</i> (<b>B</b>) and decrease of <i>9B</i> (<b>C</b>) (Cf. the two gray bars vs. white and black bars). …”

Rictor down-regulation sensitizes chemoresistant OVCA cells to CDDP-induced apoptosis by facilitating Akt proteasomal degradation. by Akechai Im-aram (5656237)

“…Rictor knock-down significantly enhanced CDDP-induced apoptosis in chemoresistant OVCA cells (P<0.01 and P<0.001, respectively) and this effect was rescued by epoxomycin (P<0.05 and p<0.001, respectively) Total-Akt was significantly decreased during rictor silencing with CDDP treatment (P<0.05) and a massive increase in the ratio between phospho-Akt (Ser473) and total-Akt was also observed, which significantly decreased when epoxomycin was added (P<0.001 and P<0.05, respectively) Results are presented as mean ± SEM of three independent experiments. …”

Feijoa F3 does not activate autophagy or inhibit NF-κB activity in autophagy defective HCT116 cells. by Noha Ahmed Nasef (760610)

“…A percentage of greater than 100 shows increased inflammatory response in the presence of the ligand when compared to media (no treatment); and a percentage of less than 100 suggests a decrease in the inflammatory response in the presence of the ligand when compared to media (no treatment) meaning that a condition has inhibited NF-κB signaling. …”

Time courses of HA-GLUT4-eGFP intensity and microtubule density increase. by Jennine M. Dawicki-McKenna (142096)

“…Adipocytes expressing HA-GLUT4-eGFP were serum-starved and pre-treated with 0 µM (dark blue, n = 13 cells) or 10 µM (light blue, n = 7 cells) nocodazole for a minimum of 20 min prior to stimulation with 100 nM insulin at t = 0 min. …”

Selective loss of macrophage subpopulations in lymphoid tissue of <i>Csf1rko</i> rats. by Dylan Carter-Cusack (20624041)

“…Positive logFC indicates an increase in expression in <i>Csf1rko</i> animals, while negative logFC indicates a decrease in expression in <i>Csf1rko</i> animals. …”

Effect of 3BrP on morphology, cell number, viability, proliferation and pluripotency. by Ana Sofia Rodrigues (491559)

“…Cells stained green were counted as live, while PI positive cells were counted as dead. 100 cells were counted per condition for a total of n = 8 independent experiments, results are expressed as means ± SEM for 30 experiments. …”

Deletion of RBPJK enhances cell spontaneous differentiation in regular MSC culture. by Xifu Shang (784529)

“…<p>Mesenchymal stem cells were infected with Control shRNA(Co) or RBPJK shRNA (shRBPJK) lentivirus before being harvested for alkaline phosphatase (ALP) staining, western blot, RT-PCR analysis and BrdU ELISA. (A) Western Blot showed a significant decrease of RBPJK protein expression at day 3 and 14 following shRBPJK lentiviral infection. …”

The alternate biochemical experiments to test for apoptosis under all the growth conditions and markers for oxidative stress. by Hung-Chih Hsu (479317)

“…HIG-82 synoviocytes (3×10⁵/100-mm cultured dishes) were treated with FC, MFD and FD for 48 h or 500 nM Epi (a positive control) for 48 h. …”

Estrogen altered the expression of cyclin D1. by Hongzhu Li (144792)

“…The cells were treated with 100 nM estrogen for 72 h. * p<0.05. <b><i>C</i></b>, Knockdown of ERα reversed estrogen-decreased expression of cyclin D1 in WT-SMCs. …”

CM₁₈-Tat₁₁/DNA plasmid complex <i>in vitro</i> transfection efficiency and cytotoxicity. by Fabrizio Salomone (438714)

“…The wst-8 reagent was added for 2 hours and absorbance at 450 nm measured. Untreated cells are defined as 100% viable, while cells exposed to 20% dimethyl sulfoxide (white column) are used as positive control for decreased metabolic activity.…”

Monitoring intracellular K⁺ concentrations with GINKO2 in <i>E</i>. <i>coli</i> grown in K⁺-depleted media. by Sheng-Yi Wu (10887927)

“…<p>(A) <i>E</i>. <i>coli</i> are capable of accumulating K⁺ to a higher concentration than the environment. …”

Myelin and myelin proteins are marginally reduced in the brain of <i>Syn1Cre; Galc flox/−</i>. by Conlan Kreher (13001753)

“…Scale bar = 100 μm. (<b>E</b>) Quantification of PLP level, measured by averaged integrated signal intensity, reveals a trend of reduction in the mutant brain. …”

High-Throughput Absolute mRNA Quantification Using Real-time RT-PCR by Anthony J Bais (13285305)

“…White and black bars indicate a respective fold increase or decrease in copy number. …”

Scale-up in the synthesis of nanoparticles for encapsulation of agroindustrial active principles by Dirliane Santos Duarte (6624989)

“…Similarly for the encapsulated acerola byproduct extract (373±44 and 400±83 nm) and ascorbic acid (279±29 and 217±29 nm). For the encapsulated skin grape extract, the size decreased sharply from 1040 to 308 nm. …”

miR-24 regulates FAF1 by targeting its ORF region. by Wenming Qin (366695)

“…(D) miR-24 regulates the FAF1 gene. 293T cells were treated as indicated and transfected with 100 ng pcDNA3.1-FAF1. The expression of FAF1 was down-regulated after transfection with 20 nM miR-24-mimic and rescued after 100 nM miR-24-ASO being transfected. …”

Combined expression of pTFs promotes transdifferentiation efficiency, however only their sequential expression increases β-cell maturation. by Dana Berneman-Zeitouni (519463)

“…<p>Cultured adult human liver cells were infected with <i>Ad-CMV-Pdx1</i> (1000 MOI), <i>Ad-CMV-Pax-4</i> (100 MOI) and <i>Ad-CMV-Mafa</i> (10 MOI) together or in a sequential manner as summarized in (A) (treatments B–E) or with control virus (<i>Ad-CMV-β-gal</i>, 1000 MOI, treatment A), and analyzed for their pancreatic differentiation six days later. …”

Supplemental Figure 1 by lsimo2@lsuhsc.edu lsimo2@lsuhsc.edu (6704447)

“…There was a main effect of OVX to decrease HOMA-β at study endpoint. …”

The effects of FRLFE on the NF-κB-dependent transcription of the <i>iNOS</i> gene. by Ryota Yamanishi (547533)

“…<p>(<b>A</b>) FRLFE decreases the iNOS promoter activity. …”

Table1_Fibrates for the Treatment of Primary Biliary Cholangitis Unresponsive to Ursodeoxycholic Acid: An Exploratory Study.docx by Guilherme Grossi Lopes Cançado (10373078)

“…</p><p>Results: In total, 27 patients (100% women, mean age 48.9 ± 9.2 years) with PBC were included. …”