From <i>in vivo</i> to <i>in vitro</i>: Major metabolic alterations take place in hepatocytes during and following isolation by Shamir Cassim (3713362)

“…Extensive metabolite measurements using HPLC were performed <i>in situ</i> in the liver, during hepatocyte isolation using the two-step collagenase perfusion method and during <i>in vitro</i> cell culture for up to 48 hours. …”

Simulations of synaptic excitation and inhibition. by Stefano Masoli (9928807)

“…(B) PSTH of responses elicited by a short train (5 impulses @100 Hz) to 20 MF, 20 AA, and 89 PF synapses. …”

Focal Ischemic Injury with Complex Middle Cerebral Artery in Stroke-Prone Spontaneously Hypertensive Rats with Loss-Of-Function in NADPH Oxidases by Hiroshi Yao (562206)

“…<div><p>By means of introgressing a loss-of-function mutation in the <i>p22phox</i> gene from the Matsumoto Eosinophilia Shinshu (MES) rat to stroke-prone spontaneously hypertensive rats (SHRSP), we constructed the SHRSP-based congenic strain lacking the P22PHOX expression (i.e., lacking NADPH oxidases [NOX] activities) (SHRSP.MES-<i>Cyba</i>^<i>mes</i>/Izm; hereafter referred to as SP.MES). …”

Isolation of Cellulose Nanofibers from <i>Oryza sativa</i> Residues via TEMPO Mediated Oxidation by Edwin S. Madivoli (8857139)

“…With the aim of utilizing cellulose nanofibrils (CNFs) from <i>Oryza sativa</i>, we isolated microcrystalline cellulose using the Kraft process, followed by successive fiber fibrillation using mechanical grinding, then (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl (TEMPO) mediated oxidation. …”

Population sorting II. by Xavier Manière (230820)

“…As expected, population with similar dynamics and intra population variability of velocities (larger µ) decrease the sorting efficiency. (C) Using the experimental data displayed on <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016637#pone-0016637-g003" target="_blank">Fig. 3</a> we computed the distribution of worms position at time τ = 100s. …”

Precipitation Evolution and Modeling of Growth Kinetics of L12-structured Al3Zr Particles in Al-0.22Zr and Al-0.32Zr (wt.%) Alloys Isothermally Aged by Pedro Henrique Lamarão Souza (4644121)

“…Peak microhardness occurred after aging at 650K for 100 h for both alloys and decreased slightly after aging at 400 h. …”

Microelectrode simulation was performed to observe the recorded SPW-Rs in different regions of neural network. by Sadullah Canakci (4439137)

“…<b>(d)</b> SPW-R amplitude recording as a function of distance, revealing that LFP amplitudes show a decrease as a function of electrode distance to the network.…”

Global mRNA Expression Profiles of <i>set1</i>Δ and <i>H3-K4R</i> Mutant Strains. by Benoit Guillemette (5650312)

“…The genes were ranked according to log₂ fold-change in the <i>H3-K4R</i> strain and a sliding average (window  =  50, step  =  1) was applied to the data on the <i>y-</i>axis. …”

Influences of IHF on DNA force response in the absence of magnesium. by Jie Lin (119522)

“…Bottom panel: force-extension curves of λ-DNA according to the Marko-Siggia formula for the protein-free DNA persistence length of 50 nm (black) and a reduced persistence length of 25 nm (red). …”

Effect of Squeeze Casting on Microhardness and Microstructure of Al-3Cu-xSi Alloy by Ronaldo Oliveira (7453877)

“…Pressures of 0 MPa, 50 MPa, 100 MPa and 150 Mpa were used. In general, with increasing pressure and composition, the λ2 values decrease from the metal/mold interface, especially at pressures of 150 MPa and Composition of 9%Si, on the other hand the increase of these variables leads to the formation of more phases Al2Cu metastable that have a decrease in the Microhardness of the material, in addition to the formation of other phases due to the proximity to the eutetic.…”

Royal jelly arranges apoptotic and oxidative stress pathways and reduces damage to liver tissues of rats by down-regulation of Bcl-2, GSK3 and NF-κB and up-regulation of caspase an... by Abdullah Aslan (9997699)

“…</p> <p><b>Results</b></p> <p>RJ caused a significant decrease in MDA level, Bcl-2, GSK3 and NF-κB protein expression levels, whereas induced a significant increase in GSH level, CAT activities and Bax, BDNF, caspase-6, caspase-3, Nrf-2 protein expression levels.…”

<i>Ramicrusta adjoulanensis</i> (GH0015334). by Matthew S. Mills (3957410)

“…Scale bar = 100 μm. (21) Section showing secondary pit connections (arrowhead) and cell fusions (arrow) in the lower perithallus, as well as the rapid decrease in cell size around the mid-perithallus. …”

Effects of DA on the I_Na. by Catalina Valdés-Baizabal (701863)

“…In this and following activation and inactivation curves the data were fitted with a Boltzmann function (solid lines). E) Concentration-response relationship of the effect of DA (1 nM to 100 μM), with at least <i>n</i> = 6 for each point. …”

The average oscillatory activities for various critical prosodies and task conditions. by Xuhai Chen (313897)

“…Red colors indicate more power increase and blue colors indicate more power decrease relative to baseline. Topographical map show data taken from a 100- to 600-ms, 4- to 6-Hz window.…”

Representative photomicrographs of vimentin-ir in the MPOA. by Natalina Salmaso (344927)

“…<p>Panel A shows vimentin-ir of the MPOA of Cycling animals, and Day 16 (MPOA- panel B, Cg2 Panel C). …”

Data_Sheet_1_Impacts of pH on the Fitness and Immune System of Pacific White Shrimp.docx by Veran Weerathunga (11522143)

“…All these results suggest that a 100-days exposure to pH 7.6 did not have a direct effect on fitness but lead to a modulation of the immune response.…”

Data_Sheet_1_Impacts of pH on the Fitness and Immune System of Pacific White Shrimp.docx by Veran Weerathunga (11522143)

“…All these results suggest that a 100-days exposure to pH 7.6 did not have a direct effect on fitness but lead to a modulation of the immune response.…”

COMMD1 mediates aggregation of ALS-associated SOD1 mutants. by Willianne I. M. Vonk (191330)

“…<p><b>A.</b> SOD1-Flag WT and mutant (A4V, G37R, G85R, D90A, G93A, E100G) constructs were co-transfected with either EV (–) or HA-COMMD1 in HEK293T cells prior to detection of SOD1 aggregates by means of filter trap analysis on cellulose acetate membrane as described in Experimental procedures section (40 μg protein; upper panel). …”

Data_Sheet_1_Biochemical, Metabolomic, and Genetic Analyses of Dephospho Coenzyme A Kinase Involved in Coenzyme A Biosynthesis in the Human Enteric Parasite Entamoeba histolytica.P... by Arif Nurkanto (6032693)

“…The E. histolytica genome encodes two DPCK isotypes (EhDPCK1 and EhDPCK2). Epigenetic gene silencing of Ehdpck1 and Ehdpck2 caused significant reduction of DPCK activity, intracellular CoA concentrations, and also led to growth retardation in vitro, suggesting importance of DPCK for CoA synthesis and proliferation. …”

Role of metabolites in mediating the effect of triacylglycerol on aplastic anemia by Jingkui Zhu (18253540)

“…</p> <p>This study aimed to examine the causal relationship between 38 different subtypes of triacylglycerols and AA using two-sample Mendelian randomization (MR). Additionally, two-step MR analyses were conducted to investigate the mediating effects of vitamin A to oleoyl-linoleoyl-glycerol (18:1–18:2) ratio.…”