Tumor Cells Switch to Mitochondrial Oxidative Phosphorylation under Radiation via mTOR-Mediated Hexokinase II Inhibition - A Warburg-Reversing Effect by Chung-Ling Lu (711442)

“…Three tumor cell lines (breast cancer MCF-7, colon cancer HCT116 and glioblastoma U87) showed a quick relocation of mTOR to mitochondria after irradiation with a single dose 5 Gy, which was companied with decreased lactate production, increased mitochondrial ATP generation and oxygen consumption. …”

Loss of <i>FGR41</i> or <i>CWP419</i> does not attenuate fungal growth <i>in vitro</i>. by Andrew S. Wagner (11331477)

Image_5_Experimental Traumatic Brain Injury Induces Chronic Glutamatergic Dysfunction in Amygdala Circuitry Known to Regulate Anxiety-Like Behavior.TIFF by Joshua A. Beitchman (8333934)

Image_5_Experimental Traumatic Brain Injury Induces Chronic Glutamatergic Dysfunction in Amygdala Circuitry Known to Regulate Anxiety-Like Behavior.TIFF by Joshua A. Beitchman (8333934)

Image_5_Experimental Traumatic Brain Injury Induces Chronic Glutamatergic Dysfunction in Amygdala Circuitry Known to Regulate Anxiety-Like Behavior.TIFF by Joshua A. Beitchman (8333934)

Image_5_Experimental Traumatic Brain Injury Induces Chronic Glutamatergic Dysfunction in Amygdala Circuitry Known to Regulate Anxiety-Like Behavior.TIFF by Joshua A. Beitchman (8333934)

Exceedance probability distribution function of species interactions and phase-space dependent on surface temperature. by Jie Li (15030)

Image_5_Extensive Healthy Donor Age/Gender Adjustments and Propensity Score Matching Reveal Physiology of Multiple Sclerosis Through Immunophenotyping.tif by Paavali A. Hannikainen (9698444)

<em>In Vitro</em> and <em>Ex Vivo</em> Analysis of <em>CHRNA3</em> and <em>CHRNA5</em> Haplotype Expression by Glenn A. Doyle (209929)

“…For <em>in vitro</em> analyses, promoter haplotypes were sub-cloned into a luciferase reporter vector. When assessed in BE(2)-C cells, luciferase expression was equivalent among <em>CHRNA3</em> haplotypes, but the combination of deletion at rs3841324 and variation at rs503464 decreased <em>CHRNA5</em> promoter-derived luciferase activity, possibly due to loss of an SP-1 and other site(s). …”

Three-dimensional flow field of the Trp-cage folding. by Vladimir A. Andryushchenko (4662829)

Induction of ELF5 alters FOXA1 and ER binding to the genome. by Catherine L. Piggin (8212272)

“…<p>Panel <b>A</b>, UpSet presentation of DiffBind output that identified statistically significant increases in FOXA1 or ER binding in response to induction of ELF5 with doxycycline (Dox). …”

Cyclophosphamide treatment globally diminishes immune cells at early timepoints. by Andrew S. Wagner (11331477)

Conformational characterization of a novel anti-HER2 candidate antibody by Leina Moro Pérez (6686177)

“…In this work, the conformational features of a novel monoclonal antibody (called 5G4) were evaluated by means of circular dichroism spectroscopy and fluorescence. …”

Functional validation of a miRNA-34a-TREM2–3’UTR interaction. by Surjyadipta Bhattacharjee (3181674)

“…<p><b>(A)</b> ribonucleotide sequence of the 299 nt TREM2-mRNA-3’-UTR is shown in the 5’-3’ direction; the 22 nt miRNA-34a-TREM2 3’UTR complementarity-interaction region is indicated by a black underline and the 8 nt TREM2-mRNA-3’-UTR seed sequence <b>5’-ACACUGCU-3’</b> is overlaid in yellow; a single arrowhead indicates the 5’ end of a poly A+ tail in the TREM2 mRNA (22 ‘A’ nt shown; the length of this poly A+ tail is variable); TREM2 mRNA sequence derived from NM_018965; TREM2 transcript is the major X1 variant (see also <a href="http://switchdb.switchgeargenomics.com/productinfo/id_801321/" target="_blank">http://switchdb.switchgeargenomics.com/productinfo/id_801321/</a>) (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a></b>); <b>(B)</b> TREM2-mRNA-3’UTR expression vector luciferase reporter assay (pLightSwitch-3’UTR; Cat#S801178; Switchgear Genomics, Palo Alto CA); in this vector, the entire 299 nucleotide TREM2 3’UTR was ligated into the unique Nhe1-Xho1 site; <b>(C)</b> control C8B4 murine microglial cells, 1 week in culture; phase contrast bright field microscopy 20x; C8B4 cells transfected with the TREM2-mRNA-3’UTR expression vector luciferase reporter were treated exogenously with miRNA-34a, a scrambled control miRNA-34a (miRNA-sc) or control miRNA-183; see references and text for further details [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref019" target="_blank">19</a>]; <b>(D)</b> compared to control, C8B4 cells transfected with a scrambled (<b>sc</b>) control pLightSwitch-3’UTR vector, the TREM2-mRNA-3‘UTR vector exhibited decreased luciferase signal to a mean of 0.16-fold of controls in the presence of miRNA-34a; this same vector exhibited no change in the presence of the control miRNA-34a-sc or miRNA-183; for each experiment (using different batches of MG cells) a control luciferase signal was generated and included separate appropriate controls with each analysis; in addition a control vector β-actin-3’UTR showed no significant effects on the relative luciferase signal yield after treatment with either miRNA-183 or miRNA-34a (data not shown); dashed horizontal line set to 1.0 for ease of comparison; N = 5; *<i>p</i><0.001 (ANOVA). …”

Key cell wall proteins mediate ß(1,3)-glucan exposure in <i>C</i>. <i>albicans</i>. by Andrew S. Wagner (11331477)

Effects of a clinical medication review focused on personal goals, quality of life, and health problems in older persons with polypharmacy: A randomised controlled trial (DREAMeR-s... by Sanne Verdoorn (6680822)

“…Over 6 months, in the intervention group, HR-QoL measured with EQ-VAS increased by 3.4 points (95% confidence interval [CI] 0.94 to 5.8; <i>p</i> = 0.006), and the number of health problems with impact on daily life decreased by 12% (difference at 6 months −0.34; 95% CI −0.62 to −0.044; <i>p</i> = 0.024) as compared with the control group. …”

Alpha- and beta-defensins are not relevant for polyP dependent gonococcal infection. by Benedetta Manca (15810991)

The influence of positional release therapy on the myofascial tension of the upper trapezius muscle by Francisco José Saavedra (4904293)

“…A significant decrease in painful symptoms from 5.3 ± 1.9 to 2.8 ± 1.8 (p < 0.001) was observed after treatment. …”

Venn diagram representing the RNA sequencing results. by Shyamasree De Majumdar (168216)

“…The arrows ⬇ indicates a lower than 0.5 fold decrease in transcription compared to calibrator; ⬆ indicates a higher than 2 fold transcription compared to calibrator. …”

PolyP pseudo-capsule protects <i>N</i>. <i>gonorrhoeae</i> from antibacterial activity of cathelicidin LL-37. by Benedetta Manca (15810991)