Variants encoding a restricted carboxy-terminal domain of <i>SLC12A2</i> cause hereditary hearing loss in humans by Hideki Mutai (723246)

“…Using whole-exome analysis of three families with congenital, severe-to-profound hearing loss, we identified a missense variant of <i>SLC12A2</i> in five affected members of one family showing a dominant inheritance mode, along with <i>de novo</i> splice-site and missense variants of <i>SLC12A2</i> in two sporadic cases, as promising candidates associated with hearing loss. …”

VDR regulates TRPV5 expression. by YongMing Chen (5104925)

“…<p>(A) RT-PCR and (B) WB were performed to examine the expression of TRPV5 following VDR overexpression or knockdown in caki-1 cells. …”

Sensitivity/specificity v.s. threshold parameter. by Lei Nie (556344)

Enumeration and characterization of 5$t-expressing CTCs. by Steven R. Pirie-Shepherd (2224690)

“…<p><b>A</b>- Characterized control cell lines representing high (H226), moderate (H1975), low (H2122) and negative (H460) 5T4 staining were prepared and spiked into healthy human blood samples as described in materials and methods. …”

Glycocalyx of BAEC monolayers cultured in 5 by Sandra V. Lopez-Quintero (486268)

“…Only the mean fluorescence representative of Heparan Sulfate was significantly decreased to 55.5±11% in HG (p = 0.023). In addition, the contents of the core proteins Syndecan-1 and Glypican-1 were determined by western blot and consistenly showed that neither high glucose nor Mannitol had an effect on these components. …”

Image_1_Network Analysis of miRNA and mRNA Changes in the Prelimbic Cortex of Rats With Chronic Neuropathic Pain: Pointing to Inflammation.TIF by Guohong Cai (490597)

“…<p>Neuropathic pain (NP) is a complex, chronic pain condition caused by injury or dysfunction affecting the somatosensory nervous system. …”

Table_1_Network Analysis of miRNA and mRNA Changes in the Prelimbic Cortex of Rats With Chronic Neuropathic Pain: Pointing to Inflammation.xlsx by Guohong Cai (490597)

“…<p>Neuropathic pain (NP) is a complex, chronic pain condition caused by injury or dysfunction affecting the somatosensory nervous system. …”

Image_2_Network Analysis of miRNA and mRNA Changes in the Prelimbic Cortex of Rats With Chronic Neuropathic Pain: Pointing to Inflammation.TIF by Guohong Cai (490597)

“…<p>Neuropathic pain (NP) is a complex, chronic pain condition caused by injury or dysfunction affecting the somatosensory nervous system. …”

Hydrolysis Mechanism of the Linkers by Matrix Metalloproteinase‑9 Using QM/MM Calculations by Binbin Chen (504446)

“…The quantum mechanics/molecular mechanics (QM/MM) calculations herein show that MMP-9 proteolysis is a water-mediated four-step reaction. More specifically, it consists of (i) nucleophilic attack, (ii) hydrogen-bond rearrangement, (iii) proton transfer, and finally (iv) amide bond rupture. …”

Force–displacement curves of M5. by Yukun Hu (427264)

Two Mutations in the Caprine <i>MTHFR</i> 3'UTR Regulated by MicroRNAs Are Associated with Milk Production Traits by Jinxing Hou (771646)

“…<div><p>Background</p><p>5,10-Methylenetetrahydrofolate reductase (MTHFR) plays a central role in folate metabolism by irreversibly converting 5,10-methylenetetrahydrofolate to 5-methylenetetrahydrofolate, a predominant circulating form of folate. …”

Expression of Fbln5 in targeted mice. by Kathleen Chin (275937)

“…<b>E.</b> Effect of Fbln5 cKO on spontaneous development of prolapse as a function of age. …”

Video_5_Contribution of 5-HT2 Receptors to the Control of the Spinal Locomotor System in Intact Rats.WMV by Henryk Majczyński (830716)

“…Little is known about the role of 5-HT receptors in the control of voluntary locomotion, so we administered inverse agonists of 5-HT₂ (Cyproheptadine; Cypr), 5-HT_2A neutral antagonist (Volinanserin; Volin), 5-HT_2C neutral antagonist (SB 242084), and 5-HT_2B/2C inverse agonist (SB 206553) receptors intrathecally in intact rats and monitored their effects on unrestrained locomotion. …”

Raw, uncropped blots for Fig 5. by Seung Ah Choi (755291)

Descriptive data of newborns enrolled in the study (n = 23). by Pedro A. R. Vanzele (17655589)

Effect of the W501R substitution on the ability of HCV helicase to unwind DNA. by Paola J. S. Provazzi (835454)

“…The reactions were prepared containing 25 mM MOPS, 1.25 mM MgCl₂, 5 nM Cy5-substrate; 1mM ATP, 0.05 mM DTT, 0.005 mg/mL BSA, 0.001% Tween20, and enzymes NS3h_1b (con1), NS3h_3a (wt) and NS3h_3a (W501R) at indicated concentrations.…”

Shared and exclusive Amplicon Sequence Variants (ASVs) before and after the oral diet implementation. by Pedro A. R. Vanzele (17655589)

Table_1_Both yields of maize and soybean and soil carbon sequestration in typical Mollisols cropland decrease under future climate change: SPACSYS simulation.docx by Shuo Liang (122569)

“…Conversely, a slight but not statistically significant average yield increase of 2.5% was predicted for spring wheat. …”

Five models of human dietary protein resources at Kosenivka using the software FRUITS, v. 3.1 [108]. by Katharina Fuchs (551018)

“…<p>1: Proportions of cereal grain protein (C) and animal meat (A) in the diet of humans from Kosenivka reconstructed from mean δ¹³C and δ¹⁵N values and SEM (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0289769#pone.0289769.t004" target="_blank">Table 4</a>). 2: Proportion of cereals calculated for the human mean (h, n = 3) as in model 1 and for individuals 5–7, arranged by decreasing δ¹⁵N in cranial bone collagen. 3: As model 2 but for the proportion of animal meat. 4, 5: Results for food source inputs to the human diet considering either δ¹³C (4) or δ¹⁵N (5) as a single fraction in the FRUITS model (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0289769#pone.0289769.s004" target="_blank">S2 Table</a>). …”

Shared and exclusive Amplicon Sequence Variants (ASVs) between the postpartum weeks (first, third, and fourth week). by Pedro A. R. Vanzele (17655589)