The NH₂-terminus of PDX-1 binds to <i>Krt19</i> promoter DNA. by Johannes von Burstin (243524)

“…C) MIN6 cells were transfected with V5-tagged full length PDX-1 or PDX-1 Δ1-37. DNA precipitation was carried out as described above, and membranes were probed with anti-V5. …”

Fig 3 - by Amanda L. Laverty (9248648)

“…Water temperature ranged from 2.1–9.5°C. Note the decreased range of the Y-axis relative to Fig 3A. …”

siRNA screening system to identify regulators of insulin promoter activity. by Gregory M. Ku (188067)

“…If the siRNA targets a positive regulator, GFP/mCherry MFI decreases. …”

Induced Wnt signaling post-SE coordinates AMPK and mTOR signaling pathways. by Roaya S. Alqurashi (11243136)

“…(n = 3–5 for each group). Data are represented as mean ± SEM. …”

Inhibition of Wnt signaling blocks adenocarcinoma cell proliferation. by Marina Pasca di Magliano (81389)

“…Transfection with an <i>Icat–IRES-eGFP</i> expression vector (<i>Icat</i>, black) or dominant negative Lef – <i>IRES-eGFP</i> (<i>dnLef</i>, gray) strongly inhibits growth of four pancreatic cancer cells lines, measured as the ability to incorporate BrdU. …”

Data_Sheet_1_SUMOylation of Alpha-Synuclein Influences on Alpha-Synuclein Aggregation Induced by Methamphetamine.doc by Lin-nan Zhu (5681939)

“…Levels of the ubiquitin-proteasome system (UPS)-related makers ubiquitin (Ub) and UbE1, as well as the autophagy-lysosome pathway (ALP)-related markers LC3, P62 and lysosomal associated membrane protein 2A (LAMP2A), were also measured in SH-SY5Y cells transfected with lentivirus and mice injected with adenovirus. …”

Image_2_P300/CBP Associated Factor (PCAF) Deficiency Enhances Diet-Induced Atherosclerosis in ApoE3*Leiden Mice via Systemic Inhibition of Regulatory T Cells.JPEG by Alwin de Jong (4012250)

“…Expression of FoxP3 by systemic CD4⁺ T cells decreased 1.8 fold in ApoE3^*LeidenxPCAF^−/− after 5 months HFD and remained significantly reduced after 5 months of HFD. …”

Molecular Characterization of Acquired Tolerance of Tumor Cells to Picropodophyllin (PPP) by Jamileh Hashemi (131207)

“…Using Nexus software analysis we combined the array-CGH data with data from gene expression profilings and identified genes that were altered in both inputs. A subset of genes identified as downregulated (<em>ALDH1A3</em>, <em>ANXA1</em>, <em>TLR4</em> and <em>RAB5A</em>) or upregulated (<em>COX6A1</em>, <em>NFIX</em>, <em>ME1</em>, <em>MAPK</em> and <em>TAP2</em>) were validated by siRNA in the tolerant or parental cells to alter sensitivity to PPP and confirmed to alter sensitivity to PPP in further cell lines.…”

Metabolomics raw data and table of extracted peak areas for BT478, BT530 and NHA cells cultured in the presence of MPA, Compound 3 (IC80) or vehicle control, for 6 hours. by Agata Monika Kieliszek (14650298)

“…The linear gradient employed was as follows: 0–2.5 min 99% A, 2.5–7.5 min decrease to 80% A, 7.5–13 min to 55% B 652 and finally 13–15 min to 99% B and held for 1 min. …”

Table_1_Regulation of miRNA expression in the prefrontal cortex by fecal microbiota transplantation in anxiety-like mice.docx by Simin Chen (9549665)

“…MiRNA analysis identified five key miRNAs affecting GAD pathogenesis, including mmu-miR-10a-5p, mmu-miR-1224-5p, mmu-miR-218-5p, mmu-miR-10b-5p, and mmu-miR-488-3p. …”

Image_1_P300/CBP Associated Factor (PCAF) Deficiency Enhances Diet-Induced Atherosclerosis in ApoE3*Leiden Mice via Systemic Inhibition of Regulatory T Cells.JPEG by Alwin de Jong (4012250)

“…Expression of FoxP3 by systemic CD4⁺ T cells decreased 1.8 fold in ApoE3^*LeidenxPCAF^−/− after 5 months HFD and remained significantly reduced after 5 months of HFD. …”

DataSheet_1_Differences in Prostate Cancer Incidence and Mortality in Lower Saxony (Germany) and Groningen Province (Netherlands): Potential Impact of Prostate-Specific Antigen Tes... by Sanny Kappen (10889607)

“…Trends in age-standardized incidence rates (ASIR) and mortality rates (ASMR) for specific age groups were assessed using joinpoint regression.</p>Results<p>The mean annual PCa ASIR between 2003 and 2012 was on average 19.9% higher in Lower Saxony than in Groningen (120.5 vs. 100.5 per 100,000), while the mean annual ASMR was on average 24.3% lower in Lower Saxony than in Groningen (21.5 vs. 28.4 per 100,000). …”

The STIM2-TM domain activates the cytosolic region of STIMs less efficiently than that of STIM1. by Sisi Zheng (1574473)

“…Left: average time course of whole-cell I_CRAC currents measured at –100 mV. Currents mediated by STIM1121-ΔK developed significantly more slowly than control (time to peak, 72 ± 7 s versus 147 ± 9 s, <i>n</i> = 7, ****<i>P</i> < 0.0001, <i>t</i> test), with the maximal current density also reduced (–5.5 ± 0.8 pA/pF versus –1.5 ± 0.1 pA/pF, <i>n</i> = 7, *****<i>P</i> < 0.0004, <i>t</i> test). …”

Effects of therapeutic and aerobic exercise programs in temporomandibular disorder-associated headaches by Paula Manuela Mendes MOLEIRINHO-ALVES (12659778)

“…Methodology Thirty-six patients diagnosed with headache attributed to TMD participated in the study and were divided into three groups of 12 patients: a therapeutic exercise program (G1, mean age: 26.3±5.6 years), a therapeutic and aerobic exercise program (G2, mean age: 26.0±4.6 years), and an aerobic exercise program (G3, 25.8±2.94 years). …”

Spatial regulation of platelet spreading. by Ashley Kita (200234)

“…On stripes of protein thinner than 5–6 µm, only a fraction of a platelet's final spread area is on protein. …”

SB-216763 inhibits the GSK-3 pathway. by Pramod K. Dash (246530)

“…<p><b>A</b>) Representative images of western blots for phospho-GSK-3ß, phospho-LRP6, phospho-ß-Catenin, phospho-Akt, and phospho-ERK in response to i.p. injection of SB-216763 (2.5 and 5.0 mg/kg) or vehicle (0 mg/kg) (n = 3/group). …”

Effects of cannabinoid receptor antagonism on pancreatic stellate cells. by Christoph W. Michalski (85727)

“…<p>(A) MTT assays after 48 hours' incubation of PSC with graded concentrations of CB1- and CB2-receptor antagonists AM251 and AM630 (p = n.s. for concentrations of 1.25, 2.5 and 5 µM). …”

Selective Adaptation in the Theoretical Model by Avner Wallach (59389)

“…For high values of <i>β</i> (here, for <i>β</i> = 0.5, 0.4, and 0.3) amplification is decreasing with stimulation frequency and is below 1 (i.e., the response to the rare is in fact depressed). …”

Silibinin and SIL inhibit HCVpp-mediated fusion. by Jessica Wagoner (148829)

“…After a 2 min-equilibration, lipid mixing was initiated by decreasing the pH to 5.0 with diluted HCl, and R₁₈ dequenching was recorded. …”

Building Strain with Large Macrocycles and Using It To Tune the Thermal Half-Lives of Hydrazone Photochromes by Quan Li (157017)

“…Strain has been used as a tool to modulate the reactivity (e.g., mechanochemistry) and thermal isomerization kinetics of photochromic compounds. …”