Multiplex genome-wide exon amplification based on MYBPC3 gene exons 7 and 8. by Periannan Senapathy (270598)

“…<p>The human genomic DNA was PCR-amplified under standard conditions at 58°C with primers designed from the donor (5′) splice signal sequence (from exon 7) and the acceptor (3′) splice signal sequence (from exon 8) of the MYBPC3 gene. …”

Impaired survival signalling in CD PMN does not affect intermediate and end-stage apoptosis. by Rajesh Somasundaram (499673)

“…(B) No differences in DNA fragmentation as measured by TUNEL assay after 15 hours of PMN culture with or without 10 ng/ml GMCSF were observed between CD and HC (n=6). (C-F) PMN were isolated simultaneously from a CD patient and healthy control, stimulated with 5 ng/ml GMCSF for 15 minutes and samples were run on one gel. …”

Swimming endurance training protocol. by Vítor S. Fernandes (21262991)

“…<i>Mib1</i>^<i>flox</i><i>;Tnnt2</i>^<i>Cre</i> cardiomyocytes also showed decreases in outward K⁺ peak currents and currents at the end of depolarizing pulses at potentials ≥−10 mV and ≥−20 mV, respectively, and this was accompanied by a lower charge density at ≥−20 mV. …”

Tuning inhibition to alter the cortical state. by Leila Fakhraei (4002038)

“…<p>For both our experiment (a-c) and our model (d-f), we studied a range of cortical states characterized at one extreme by asynchronous firing and low amplitude LFP (a, d) and at the other extreme by firing synchrony and large amplitude LFP (c, f). …”

Data_Sheet_1_Regulation of the cecal microbiota community and the fatty liver deposition by the addition of brewers’ spent grain to feed of Landes geese.ZIP by Ping Xu (122619)

“…The results showed that during the rearing stage, the body weight (BW) and the average daily gain (ADG) of Landes geese were significantly increased in groups F and W, while during the overfeeding stage, the liver weights of groups W and B were significantly higher than that of group C. The taxonomic structure of the intestinal microbiota revealed that during the overfeeding period, the relative abundance of Bacteroides in group W was increased compared to group C, while the relative abundances of Escherichia–Shigella and prevotellaceae_Ga6A1_group were decreased. …”

Combined treatment of CAPE with chemotherapy drugs shows synergistic and antagonistic inhibition on proliferation of PC-3 cells. by Hui-Ping Lin (184894)

“…<p>Proliferation of PC-3 cells treated with increasing dosage (0, 5, 10, 20 µM) of CAPE in combination with increasing concentration of etoposide (A), paclitaxol (B), vinblastine (C), mitoxantrone (D), and estramustine (E) for 72 h was determined by 96-well proliferation assay. …”

Quantitative analysis of the collagen fibers in the subcutaneous pancreatic tumor xenografts. by Wenyan Hu (162244)

“…<p>(A) The collagen density decreases as the tumor xenografts grow. …”

Single-driver controllability of brain networks. by Remy Ben Messaoud (20521877)

“…Each point corresponds to a different node (ROI) controlling the entire brain. …”

Temporal changes in mRNA abundance reflect evolving biological processes during MC3T3-E1 differentiation. by Louis M. Luttrell (408469)

“…<p>The mRNA abundance of 976 significantly regulated genes identified by ANOVA as changing during MC3T3-E1 differentiation was used to calculate expression ratios comparing D2 vs D5, D5 vs D10, and D10 vs D28. For each pairwise comparison, the earlier time point was used as the denominator and later time point as the numerator, such that expression ratios reflect increases/decreases in mRNA abundance as differentiation proceeds. …”

Mitochondrial dysfunction and atrophy in the <i>Irp2-</i>null mice. by Suh Young Jeong (201871)

“…C; The mitochondrial proteins SDH-A, SDH-B and ferrochelatase were significantly decreased in <i>Irp2</i>-null mice, whereas citrate synthase, which does not contain a [Fe-S] cluster, did not change and was used as a mitochondrial loading control. n = 5 **; p<0.001 compared to wildtype, analyzed by one-way ANOVA, D; TEM showed mitochondrial vacuolization (arrows) in axons in the ventral white matter of <i>Irp2</i>-null mice and abnormal bundling of neurofilaments within axons was also noted (arrowhead). …”

GSL-1 treatment induces transcripts for GM3 and GD3 synthases and the anti-apoptotic protein Bcl-2. by Rafael L. Pereira (177990)

“…Because GM3 gangliosides were associated with resistance to ADM-cytotoxicity due to Bcl-2 induction, we measured the mRNA levels of this protein in our system. (C) Bcl-2 transcripts were not altered by ADM but increased in ADM+GSL-1 mice, indicating a relationship between increased ST3Galt5 and Bcl-2 transcripts with renal protection from effects of ADM. * p<0.05 vs. control; # p<0.05 vs. …”

Topological changes of the Gαs protein upon β₂AR binding. by Min Hyeok Kim (2071243)

“…(B) Topological change (displacement and torsional angle) of Gαs upon β₂AR binding. (C) The receptor-binding interface of Gαs. The distance between the α5 helix and α4-β6 decreases to 7 Å, which is within the interaction range. …”

Extracellular acidification delays neutrophil apoptosis and reduces caspase 3 activation. by Shannan Cao (792360)

“…Cell death was assessed by FACS analysis with annexin V and PI staining. At least three separate experiments were carried out with a minimum of 10,000 cells counted per data point. …”

ELECTROMYOGRAPHY AND INSTRUMENTATION IN PATIENTS WITH IDIOPATHIC SCOLIOSIS by ANDERSON ALVES DIAS (12812008)

“…It enables to check if the screw is located on the correct path, helping to decrease the error rate and providing corrections to the surgical approach through a change of strategies. …”

FGFR1-Frs2/3 pathway is required for the specification of prosensory domain. by Kazuya Ono (513633)

“…<p>(A–C) p27^Kip1 (magenta) is expressed in the post-mitotic, BrdU (green) negative, prosensory domain (bracket) in sections of E14.5 cochlea (A). …”

Data Sheet 1_Pregnancy and contraceptive use among participants of childbearing potential in the HVTN 705 HIV vaccine trial in Southern Africa.docx by Pamela Mda (20390050)

“…</p>Methods<p>We performed an exploratory analysis of data collected for HVTN 705/HPX2008, a phase IIb HIV vaccine trial enrolling cisgender women across 23 sites in five southern African countries. …”

Comparison of the fraction of cooperators for different behavioral updating. by Xiaochen He (455300)

“…Other parameters are <i>I</i>_max = 1000, <i>β</i> = 10, <i>ω</i> = 0.5, <i>γ</i> = 0.5 and <i>λ</i> = 0.5. Since the curves give average values, most of simulations may evolve to 0 or 1 when not considering of structural balance, and a higher <i>τ</i> may guarantee a higher probability for the cooperators evolve to 100%. …”

Evaluation of STL properties. by Szymon Stoma (269909)

“…<p>Temporal evolution of the ratio <i>cPARP/PARP_total</i>, of the value of the property <i>alive</i>: = <i>cPARP/PARP_total<0.5</i>, and of <i>Property 1</i>: = <i>always_[0–6h]( cPARP/PARP_total<0.5</i>) for HCT116 and OE-Bcl2 HCT116 cells (left), and SKW6.4 and OE-Bcl2 SKW6.4 cells (right). …”

The phosphomimetic eIF2α variant increases clonogenic death in control and irradiated cells: by Liron Tuval-Kochen (476856)

“…At this concentration SD decreased clonogenic survival. <b>b</b>,<b>c</b>. Cells transfected with 1.5µg/2ml SA or with SD. …”

Fig 4 - by Richard D. Lange (11768146)

“…This is analogous to, for example, an orientation-discrimination task with randomized phases, such that the <i>average</i> stimulus in pixel-space is identical for both categories. While panel (v) (precise model) shows a slight increase in variance in the dp_b(<b>x</b>|…)/d<i>s</i> direction, consistent with (f) and (k), panel (q) (imprecise model) shows a slight <i>decrease</i>.…”