Purification and characterization of His-tagged PhaZ_UD. by Tomohiro Morohoshi (5735813)

“…(B) The PHBH-degrading activity of His-tagged PhaZ_UD. A total of 200 μL of purified PhaZ_UD sample was mixed with 800 μL of 5 mg/mL PHBH solution in a 1.5 mL microtube. …”

Heat map of the miRNAs that are regulated at different times following MCAo. by Fu Jia Liu (422650)

“…Green: downregulation; Red: upregulation. (<b>A</b>) The expression of 334 miRNAs that are statistically significant are represented in the heat map; (<b>B</b>) Cluster of miRNAs that show a decrease in expression during the progression of cerebral ischemia (rno-miR-196a, -196b, -196c, -224, -324-3p); (<b>C</b>) Cluster of miRNAs that show an increase in expression during the progression of cerebral ischemia (rno-miR-21, -142-3p/5p, -146a); (<b>D</b>) Cluster of miRNAs that show an expression which positively correlated with infarct volume during the progression of cerebral ischemia (rno-miR-206, -290, -291a-5p and -30c-1*).…”

Substrate binding specificity of purified OpuAC. by Justina C. Wolters (207763)

“…<p>Binding was measured using intrinsic protein fluorescence at an OpuAC concentration of ∼0.5 µM. The following substrates were tested: proline (panel A), carnitine (panel B), proline betaine (panel C) and glycine betaine (Panel D–F). …”

Changes in epidemiology of conjunctivitis in Hospital Banco de Olhos de Porto Alegre after the outbreak of the COVID-19 pandemic by Letícia Frossard de Assis Bocchese (16488662)

“…In the same period of 2020, this number was 14.194, a drop of 34.5%. The rate of visits having conjunctivitis as main cause also dropped significantly. …”

HIV/AIDS case definition criteria and association between sociodemographic and clinical aspects of the disease reported in the State of Minas Gerais from 2007 to 2016 by Sybelle de Souza Castro (5668175)

“…By contrast, a consequent decrease was observed in the number of criteria used for defining cases, which were adapted from the Centers for Disease Control and Prevention, Rio de Janeiro/Caracas, and for identifying AIDS-related deaths. …”

Changes in human intervertebral disc biochemical composition and bony end plates between middle and old age by Delio Eulalio Martins (5759282)

“…</p><p>Results</p><p>The old-aged group showed a decrease in content of sulphated and non-sulphated glycosaminoglycans relative to middle-aged and there were also alterations in the proportion of GAG disaccharides and a decrease of collagen fiber size. …”

Data_Sheet_1_Climate Warming Increased Spring Leaf-Out Variation Across Temperate Trees in China.pdf by Yaru Zhang (484892)

“…Furthermore, the LOV changes between 1987–2008 and 1963–1986 were significantly smaller at high latitudes (average decrease of 1.0 day) than at low latitudes (average increase of 1.5 days). …”

Acute Toxicity of Silver Free and Encapsulated in Nanosized Zeolite for Eukaryotic Cells by Clément Anfray (3923939)

“…After exposure to Ag⁺-EMT (50 μg/mL) for 24 h, a loss in cell viability independent of the cell type was observed, ranging from −34.37 ± 23.90% for astrocytes to −99.5 ± 0.24% for U87-MG cells. …”

Donor and Geometry Optimization: Fresh Perspectives for the Design of Polyoxometalate Charge Transfer Chromophores by Bethany R. Hood (20396768)

“…The linear systems show that with julolidinyl (Jd) and −NTol₂ donor groups, the alkyne bridge yields high second-order nonlinear optical (NLO) coefficients β (Jd, β_0,<i>zzz</i> = 318 × 10^–30 esu; −NTol₂, β_0,<i>zzz</i> = 222 × 10^–30 esu), indeed the Jd compound gives the highest NLO activity of any organoimido-POM to date with minimal decrease in transparency. The <i>bis</i>-functionalized 2D (<i>C</i>_2v) POM derivative showed increased activity over its monofunctionalized analogue with no decrease in transparency, although the NLO response was only minimally two dimensional. …”

Data_Sheet_1_Current Perspectives on Achieving Pronounced Enteric Methane Mitigation From Ruminant Production.docx by Emilio M. Ungerfeld (5414681)

“…<p>Limiting global warming to 1.5°C above pre-industrial levels by 2050 requires achieving net zero emissions of greenhouse gases by 2050 and a strong decrease in methane (CH₄) emissions. …”

ORC2 silencing enhanced PV replication in proliferating and differentiating CIN612-9E cells. by Marsha DeSmet (2513767)

“…<p>(A) ORC2 siRNA duplexes decreased ORC2 protein levels at a concentration of 15 or 5 nM after 48 h. …”

Alterations of Alix ko synapses revealed in hippocampal slices and in an <i>in vivo</i> model of epilepsy. by Marine H. Laporte (12770674)

“…<p>(<b>A</b>) Emx1 ^IREScre (Emx-Cre) and Alix ^fl/fl mouse lines were crossbred to delete Alix in neocortical and hippocampal excitatory neurons (Alix cko). …”

HDAC1 acts in tandem with T antigen to antagonize the transcriptional activity of CBP by Ester Valls (29650)

“…The data represent an average of at least five independent transfections. A schematic representation of the constructs used is shown at the bottom of the figure. …”

Image1_Identification of miRNAs related to osteoporosis by high-throughput sequencing.jpeg by Jiachun Huang (19350616)

“…The qRT-PCR results showed that miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542-3p exhibited a decrease of 2.27-, 3.00-, 3.48-, 2.67-, 2.41-, and 1.98-fold (all P < 0.05) in osteoporosis compared to controls. …”

NDPK inhibits exocytosis. by Sarah J. Annesley (202479)

“…<p>Duplicate measurements of the rate of exocytosis of FITC-Dextran were made in three independent experiments and the mean for each transformant plotted against the NDPK expression index (defined as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0026024#pone-0026024-g005" target="_blank">Fig. 5</a>). …”

Surface roughness and mechanical testing of sintered nylon and PCL. by Ian S. Kinstlinger (2164438)

“…<p>a) Surface roughness (R_a) of PCL decreases nearly 30-fold as a result of vapor-smoothing. …”

The effect of life context. by Daniel Austin (528416)

“…(A) Mobility declines with increasing age, where expected mobility decreases by a factor of 0.83 as age increases from 71 to 97 years. …”

An automated quantitative analysis of cell, nucleus and focal adhesion morphology by Antonetta B. C. Buskermolen (5043563)

Synaptic potentiation reduces AP onset, threshold and AHPs. by Sung-Cherl Jung (261358)

“…Examples of APs traces recorded before (black) and 30 min (red) after conditioning stimulation are given for paired and unpaired conditions. Scale bars: 40 mV, 200 ms. (right panel) Pooled data. The threshold to fire the first AP was lowered initially after LTP induction and this decrease persisted throughout the recording episode. …”

Immune Profiles to Predict Response to Desensitization Therapy in Highly HLA-Sensitized Kidney Transplant Candidates by Julie M. Yabu (3174753)

“…Responders to desensitization therapy were defined as 5% or greater decrease in cumulative calculated panel reactive antibody (cPRA) levels, and non-responders had 0% decrease in cPRA. …”