Tumor Cells Switch to Mitochondrial Oxidative Phosphorylation under Radiation via mTOR-Mediated Hexokinase II Inhibition - A Warburg-Reversing Effect by Chung-Ling Lu (711442)

“…Three tumor cell lines (breast cancer MCF-7, colon cancer HCT116 and glioblastoma U87) showed a quick relocation of mTOR to mitochondria after irradiation with a single dose 5 Gy, which was companied with decreased lactate production, increased mitochondrial ATP generation and oxygen consumption. …”

Multi-Cilia Cell Hyperplasia Is Due to Mib-Mediated Jagged2a Signaling Pathway via Notch1a and Notch3 Receptors by Ming Ma (43147)

“…RT-PCR of control embryos generates a 230-bp <i>jagged2a</i> fragment, bridging parts of exon 1 and exon 2 at 24 hpf (lane 1) and 48 hpf (lane 5). …”

Conformational characterization of a novel anti-HER2 candidate antibody by Leina Moro Pérez (6686177)

“…In this work, the conformational features of a novel monoclonal antibody (called 5G4) were evaluated by means of circular dichroism spectroscopy and fluorescence. …”

Image_5_Extensive Healthy Donor Age/Gender Adjustments and Propensity Score Matching Reveal Physiology of Multiple Sclerosis Through Immunophenotyping.tif by Paavali A. Hannikainen (9698444)

<em>In Vitro</em> and <em>Ex Vivo</em> Analysis of <em>CHRNA3</em> and <em>CHRNA5</em> Haplotype Expression by Glenn A. Doyle (209929)

“…For <em>in vitro</em> analyses, promoter haplotypes were sub-cloned into a luciferase reporter vector. When assessed in BE(2)-C cells, luciferase expression was equivalent among <em>CHRNA3</em> haplotypes, but the combination of deletion at rs3841324 and variation at rs503464 decreased <em>CHRNA5</em> promoter-derived luciferase activity, possibly due to loss of an SP-1 and other site(s). …”

2-DE gels of <i>A. brasilense</i> Sp7 and Sp7-flcAΔ under flocculation conditions. by Xingsheng Hou (674000)

Generating resistant and susceptible colonies with a similar genetic background. by Kelsey L. Adams (12859609)

Overexpression of tau inhibited whole-cell currents through Kv channels. by Xiantao Li (683919)

“…<p>(A) The outward Kv currents were evoked by stepped up to +60 mV for 500 ms at a holding potential of -80 mV in N2A cells. …”

Strain-Insensitive, Crosstalk-Suppressed, Ultrawide-Linearity Iontronic Tactile Skin from a Synergistic Segment-Embedded Strategy by Yanchao Zhao (5129174)

Strain-Insensitive, Crosstalk-Suppressed, Ultrawide-Linearity Iontronic Tactile Skin from a Synergistic Segment-Embedded Strategy by Yanchao Zhao (5129174)

Changes in ELF5, FOXA1 and ER binding to the genome are reflected in gene expression. by Catherine L. Piggin (8212272)

“…Panel <b>B</b>, as for panel A but showing genes for which FOXA1 or ER binding was increased or decreased by induction of ELF5. …”

ELF5 transcriptional effects measured by RNA-seq. by Catherine L. Piggin (8212272)

“…<p>Panel <b>A</b>, GO ontologies enriched in the RNA-seq data set by induction of ELF5, font size relative to enrichment score. …”

The MITF-SOX10 regulated long non-coding RNA DIRC3 is a melanoma tumour suppressor by Elizabeth A. Coe (8195508)

“…<i>DIRC3</i> is a nuclear lncRNA that activates expression of its neighbouring <i>IGFBP5</i> tumour suppressor through modulating chromatin structure and suppressing SOX10 binding to putative regulatory elements within the <i>DIRC3</i> locus. …”

Reaction force vs. 5 µm displacement of the indenter. by Shreyas S. Rao (170163)

“…<p>Insert illustrates a decrease in stiffness with increasing Matrigel height due to a 5 µm indenter displacement. …”

Disruption of the viral cyclin has no effect on the frequency of viral genome positive cells yet results in a reduced frequency of LANA::βla+ cells. by Brian F. Niemeyer (11707995)

ADAR1 binding affinity at inferred A-to-I RNA editing candidate sites. by Xinxin Peng (219357)

Functional validation of a miRNA-34a-TREM2–3’UTR interaction. by Surjyadipta Bhattacharjee (3181674)

“…<p><b>(A)</b> ribonucleotide sequence of the 299 nt TREM2-mRNA-3’-UTR is shown in the 5’-3’ direction; the 22 nt miRNA-34a-TREM2 3’UTR complementarity-interaction region is indicated by a black underline and the 8 nt TREM2-mRNA-3’-UTR seed sequence <b>5’-ACACUGCU-3’</b> is overlaid in yellow; a single arrowhead indicates the 5’ end of a poly A+ tail in the TREM2 mRNA (22 ‘A’ nt shown; the length of this poly A+ tail is variable); TREM2 mRNA sequence derived from NM_018965; TREM2 transcript is the major X1 variant (see also <a href="http://switchdb.switchgeargenomics.com/productinfo/id_801321/" target="_blank">http://switchdb.switchgeargenomics.com/productinfo/id_801321/</a>) (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a></b>); <b>(B)</b> TREM2-mRNA-3’UTR expression vector luciferase reporter assay (pLightSwitch-3’UTR; Cat#S801178; Switchgear Genomics, Palo Alto CA); in this vector, the entire 299 nucleotide TREM2 3’UTR was ligated into the unique Nhe1-Xho1 site; <b>(C)</b> control C8B4 murine microglial cells, 1 week in culture; phase contrast bright field microscopy 20x; C8B4 cells transfected with the TREM2-mRNA-3’UTR expression vector luciferase reporter were treated exogenously with miRNA-34a, a scrambled control miRNA-34a (miRNA-sc) or control miRNA-183; see references and text for further details [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref019" target="_blank">19</a>]; <b>(D)</b> compared to control, C8B4 cells transfected with a scrambled (<b>sc</b>) control pLightSwitch-3’UTR vector, the TREM2-mRNA-3‘UTR vector exhibited decreased luciferase signal to a mean of 0.16-fold of controls in the presence of miRNA-34a; this same vector exhibited no change in the presence of the control miRNA-34a-sc or miRNA-183; for each experiment (using different batches of MG cells) a control luciferase signal was generated and included separate appropriate controls with each analysis; in addition a control vector β-actin-3’UTR showed no significant effects on the relative luciferase signal yield after treatment with either miRNA-183 or miRNA-34a (data not shown); dashed horizontal line set to 1.0 for ease of comparison; N = 5; *<i>p</i><0.001 (ANOVA). …”

Interventions for Psychological Stress in Pregnant African American Women: A Scoping Review by Taleah A. Frazier (22423294)

Additional <i>Setd5</i> behavioural controls. by Laura E. Burnett (18794525)

“…<b>(A</b>) Average speed during prestimulus exploration (<i>Setd5</i>^+/+, 7.50 cm s⁻¹; <i>Setd5</i>^+/−, 6.65 cm s⁻¹, <i>P</i> = 0.151). …”

Model comparison for different theories for the mouse germline bottleneck. by David M. Edwards (10692265)