DataSheet_2_Molecular characterization of cross-kingdom RNA interference in Botrytis cinerea by tomato small RNAs.xlsx by Si Qin (3627863)

“…In order to study the functionality of this plant sRNA in reducing the Bcspl1 transcript level, we generated a fungal mutant that contained a 5-nucleotide substitution that would abolish the interaction between the transcript and the sRNA without changing the encoded protein sequence. …”

Modelling of the effects of En1 and En2 on hippocampal cells. by Asma Soltani (4356841)

“…In normal conditions (black lines), low levels of endogenous secreted En1 (from GABA cell for instance) could participate to dendritogenesis (1), spinogenesis (2) (insert), and mTOR signaling (3) in neighbor glutamatergic neurons. In a pathological context, such as autism (red lines), a rise of En2 expression and secretion would generate additional effects, i.e. the increase in dendritic complexity of GABA cells (4), a decrease of clustered PSD95 (5), and the mispairing of glutamatergic synapses (6) (insert). …”

cGMP-Dependent Protein Kinase Type I Is Implicated in the Regulation of the Timing and Quality of Sleep and Wakefulness by Sonja Langmesser (57421)

“…Moreover, PRKG1 deficiency results in an aberrant pattern, and consequently a reduced quality, of sleep and wakefulness, possibly due to a decreased wake-promoting output of the circadian system impinging upon sleep.…”

Altered Cerebrospinal Fluid Proteins in Smith–Lemli–Opitz Syndrome by Wenping Li (440398)

Olfactory Bulb Glomerular NMDA Receptors Mediate Olfactory Nerve Potentiation and Odor Preference Learning in the Neonate Rat by Rebecca Lethbridge (173780)

“…<em>Ex vivo</em> experiments using bulbs from trained rat pups reveal an increase in the AMPA/NMDA EPSC ratio post-training, consistent with an increase in AMPA receptor insertion and/or the decrease in NMDAR subunits. These results support a model of a cAMP/NMDA interaction in generating rat pup odor preference learning.…”

Use of 2-Aminopurine Fluorescence To Examine Conformational Changes during Nucleotide Incorporation by DNA Polymerase I (Klenow Fragment)^† by Vandana Purohit (2947848)

“…Finally, when the primer had a 3‘-OH, a fluorescence decrease with a rate equal to the rate of nucleotide incorporation was observed with both 0 and +1 position reporters. …”

An immunohistochemically stained photomicrograph of the stomach mucosa using an anti-TLR4 antibody. by Rehab F. Taher (20478600)

“…<p>(a) few TLR4-positively stained cells in the normal group; (b) the control ethanol group showed elevated TLR4 expression and a higher proportion of positive cells having deep brown coloring, (c) Omeprazole group showed reduction in the percentage of TLR4 positive cells with strong brown coloring, (d) PM-EtOH (25 mg/kg) group showed lowered percentage of TLR4 positive cells with weak cytoplasmic staining, (e) PM-EtOH (5o mg/kg) group showed decreased percentage of TLR4 positive cells, (f) PM-EtOH (100 mg/kg) group giving weak TLR4 expression, with a significant decrease in TLR4-positively stained cells(TLR4 immunohistochemical staining; Scale bar=100µm).…”

An Inducible Cell-Cell Fusion System with Integrated Ability to Measure the Efficiency and Specificity of HIV-1 Entry Inhibitors by Alon Herschhorn (198447)

“…Target cells express the HIV-1 receptors, CD4 and CCR5, and carry the firefly luciferase (F-Luc) reporter gene under the control of a tTA-responsive promoter. …”

Circular Dichroism analysis, PPIase activity and nickel regulation of <i>H</i>. <i>pylori</i> WT and mutant SlyD proteins. by Milica Denic (9987685)

“…<p><b>A.</b> Circular Dichroism spectra of SlyD wild type and mutant proteins were analyzed in 5 mM phosphate buffer (pH = 7.6). …”

Altered Cerebrospinal Fluid Proteins in Smith–Lemli–Opitz Syndrome by Wenping Li (440398)

Representative kinetic traces illustrating the JRA-associated abnormality in metabolic oscillations of neutrophils by James N Jarvis (3342)

“…However, in contrast to MPO-negative cells, MPO-positive cells undergo both a decrease in period to 10 seconds and a dramatic increase in the oscillatory amplitude. …”

Changes in Expression Levels of miR-1 and miR-133 and their targets in canine HF Myocytes. by Andriy E. Belevych (338034)

“…*p<0.05, n = 5. C. Representative Western blots of putative targets of miR-1 and -133, regulatory subunits B56α, B56δ and catalytic subunit of PP2A respectively. …”

Lab 3: Biol2050 Abundance and Diversity in Insects Data Set 1 by Katrin Chapkis (797943)

“…When the wind speed was found in the Beaufort scale, it was given a Beaufort number of 5, which corresponded to Fresh Breeze in Seaman's term.…”

Use of 2-Aminopurine Fluorescence To Examine Conformational Changes during Nucleotide Incorporation by DNA Polymerase I (Klenow Fragment)^† by Vandana Purohit (2947848)

“…Finally, when the primer had a 3‘-OH, a fluorescence decrease with a rate equal to the rate of nucleotide incorporation was observed with both 0 and +1 position reporters. …”

Use of 2-Aminopurine Fluorescence To Examine Conformational Changes during Nucleotide Incorporation by DNA Polymerase I (Klenow Fragment)^† by Vandana Purohit (2947848)

“…Finally, when the primer had a 3‘-OH, a fluorescence decrease with a rate equal to the rate of nucleotide incorporation was observed with both 0 and +1 position reporters. …”

Table_2_Characteristics of steroid hormones in systemic lupus erythematosus revealed by GC/MS-based metabolic profiling.docx by Dehong Wu (2236318)

“…Principle component analysis demonstrated that the steroid profile was obviously distinguished between patients with SLE and controls. A lower level of total androgens was observed in patients, and nine androgens [dehydroepiandrosterone (DHEA), testosterone, Etio, androsterone, βαβ-Diol, Epi-An, Epi-DHT, 16α-OH-DHEA, and A-Diol] underwent significant decrease. …”

Mechanical Properties of Water-Assembled Graphene Oxide Langmuir Monolayers: Guiding Controlled Transfer by Katharine L. Harrison (1532038)

SPARC effect on ECM proteins in differentiating myoblasts. by Aicha Melouane (4530787)

“…A decrease of fibronectin expression with rSPARC and an increase after anti-SPARC were observed. …”

Scaling of Soaring Seabirds and Implications for Flight Abilities of Giant Pterosaurs by Katsufumi Sato (115898)

“…In comparisons of the power available from muscle and the mechanical power required to fly, it is predicted that the margin between the powers should decrease with body size and that flying animals have a maximum body size. …”

Differentially expressed genes by the risk alleles at 29 Mb and 33 Mb play important role in T-cell immunity. by Noriko Tonomura (227263)

“…<p>A. The risk allele at the 29 Mb at homozygous state has a clear cis-regulation effect on the expression levels of <i>TRPC6</i>, <i>KIAA1377</i>, and <i>ANGPTL5</i>, three of the most proximal genes. …”