Induction of ELF5 alters FOXA1 and ER binding to the genome. by Catherine L. Piggin (8212272)

“…<p>Panel <b>A</b>, UpSet presentation of DiffBind output that identified statistically significant increases in FOXA1 or ER binding in response to induction of ELF5 with doxycycline (Dox). …”

Abundance-interaction atemporal pattern and distribution of species interactions for competitive and cooperative species. by Jie Li (15030)

Cyclophosphamide treatment globally diminishes immune cells at early timepoints. by Andrew S. Wagner (11331477)

Conformational characterization of a novel anti-HER2 candidate antibody by Leina Moro Pérez (6686177)

“…In this work, the conformational features of a novel monoclonal antibody (called 5G4) were evaluated by means of circular dichroism spectroscopy and fluorescence. …”

Functional validation of a miRNA-34a-TREM2–3’UTR interaction. by Surjyadipta Bhattacharjee (3181674)

“…<p><b>(A)</b> ribonucleotide sequence of the 299 nt TREM2-mRNA-3’-UTR is shown in the 5’-3’ direction; the 22 nt miRNA-34a-TREM2 3’UTR complementarity-interaction region is indicated by a black underline and the 8 nt TREM2-mRNA-3’-UTR seed sequence <b>5’-ACACUGCU-3’</b> is overlaid in yellow; a single arrowhead indicates the 5’ end of a poly A+ tail in the TREM2 mRNA (22 ‘A’ nt shown; the length of this poly A+ tail is variable); TREM2 mRNA sequence derived from NM_018965; TREM2 transcript is the major X1 variant (see also <a href="http://switchdb.switchgeargenomics.com/productinfo/id_801321/" target="_blank">http://switchdb.switchgeargenomics.com/productinfo/id_801321/</a>) (<b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.g003" target="_blank">Fig 3</a></b>); <b>(B)</b> TREM2-mRNA-3’UTR expression vector luciferase reporter assay (pLightSwitch-3’UTR; Cat#S801178; Switchgear Genomics, Palo Alto CA); in this vector, the entire 299 nucleotide TREM2 3’UTR was ligated into the unique Nhe1-Xho1 site; <b>(C)</b> control C8B4 murine microglial cells, 1 week in culture; phase contrast bright field microscopy 20x; C8B4 cells transfected with the TREM2-mRNA-3’UTR expression vector luciferase reporter were treated exogenously with miRNA-34a, a scrambled control miRNA-34a (miRNA-sc) or control miRNA-183; see references and text for further details [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref018" target="_blank">18</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0150211#pone.0150211.ref019" target="_blank">19</a>]; <b>(D)</b> compared to control, C8B4 cells transfected with a scrambled (<b>sc</b>) control pLightSwitch-3’UTR vector, the TREM2-mRNA-3‘UTR vector exhibited decreased luciferase signal to a mean of 0.16-fold of controls in the presence of miRNA-34a; this same vector exhibited no change in the presence of the control miRNA-34a-sc or miRNA-183; for each experiment (using different batches of MG cells) a control luciferase signal was generated and included separate appropriate controls with each analysis; in addition a control vector β-actin-3’UTR showed no significant effects on the relative luciferase signal yield after treatment with either miRNA-183 or miRNA-34a (data not shown); dashed horizontal line set to 1.0 for ease of comparison; N = 5; *<i>p</i><0.001 (ANOVA). …”

Key cell wall proteins mediate ß(1,3)-glucan exposure in <i>C</i>. <i>albicans</i>. by Andrew S. Wagner (11331477)

Alpha- and beta-defensins are not relevant for polyP dependent gonococcal infection. by Benedetta Manca (15810991)

Venn diagram representing the RNA sequencing results. by Shyamasree De Majumdar (168216)

“…The arrows ⬇ indicates a lower than 0.5 fold decrease in transcription compared to calibrator; ⬆ indicates a higher than 2 fold transcription compared to calibrator. …”

PolyP pseudo-capsule protects <i>N</i>. <i>gonorrhoeae</i> from antibacterial activity of cathelicidin LL-37. by Benedetta Manca (15810991)

Generating resistant and susceptible colonies with a similar genetic background. by Kelsey L. Adams (12859609)

LOS sialylation does not compete with polyP for the protection from cathelicidin LL-37. by Benedetta Manca (15810991)

Functional TRPM7 channel is expressed in mouse cortical astrocytes. by Zhao Zeng (478844)

“…<p>A. Voltage ramp (-100 to +100 mV) was applied for 4s from a holding potential of −60 mV. …”

Figure S5 - Heatmap of genes in Arginine and Proline Metabolism by Scott G. Daniel (4051720)

Examples of the data provided by the Kinect. by Stella C. Gerdemann (12436680)

Ternary plot of the ash forming elements in the biomass samples—using the approach proposed by Vassilev et al. [23]. by Trevor James Morgan (787777)

The MITF-SOX10 regulated long non-coding RNA DIRC3 is a melanoma tumour suppressor by Elizabeth A. Coe (8195508)

“…<i>DIRC3</i> is a nuclear lncRNA that activates expression of its neighbouring <i>IGFBP5</i> tumour suppressor through modulating chromatin structure and suppressing SOX10 binding to putative regulatory elements within the <i>DIRC3</i> locus. …”

Physico-chemical characterization of PvTRAg33.5. by Hema Bora (141649)

“…<p>Far UV CD spectra at different pH (a), emission wavelength at maximum intensity after exciting it at 295 nm as a function of pH (b), fluorescence intensity (filled circle) and emission wavelength at maximum intensity (empty circle) (inset) of ANS-protein complex as a function of pH, after exciting it at 380 nm (c), fluorescence intensities and emission wavelength maxima (inset) after exciting at 295 nm as a function of increase in temperature (empty circle) and consecutive decrease in temperature (filled circle) at pH 7.0 (d), far UV CD spectra and fluorescence intensities (inset) in the absence and presence of different ammonium sulfate concentrations at pH 7.0 after excited at 295 nm (e), and far UV CD spectra and fluorescence intensities (inset) in the absence (N) and presence of different ethanol concentrations after exciting at 295 nm (f) of PvTRAg33.5.…”

<strong>At-home use of a pregnancy-specific Zone-MPC closed-loop system for pregnancies complicated by type 1 diabetes: a single arm, observational multicenter study</strong> by Carol J. Levy (15369802)

“…</p> <p>Results: Ten participants (HbA1c 5.8±0.6%) used the system from mean gestational age of 23.7±3.5 weeks. …”

Ternary plot of the compositional analysis of biomass samples examined in this study—using the approach proposed by Vassilev et al. [23]. by Trevor James Morgan (787777)

Model comparison for different theories for the mouse germline bottleneck. by David M. Edwards (10692265)