Detection of DNA-DNA and RNA-DNA triplexes by EMSA and NMR, and molecular modeling of miRNA-duplex DNA triplex. by Steven W. Paugh (780960)

“…<p><b>(A)</b> EMSA; 5’ ROX-labeled hairpin duplex DNA (0.1 μM) was incubated for 3-hrs at 22°C in the presence (lanes 2–11) or absence (lane 1) of 2.5 μM 483-opti DNA oligo, and increasing concentration (30, 60, 150 μM) of Hoogsteen bond-optimized hsa-miR-483-5p (483-opti, lanes 3–5), hsa-miR-483-5 (483, lanes 6–8), or a scrambled RNA oligo (Scramble, lanes 9–11). …”

Modeling the RA Morphogen Gradient by Richard J White (82003)

“…Values of parameters that differed between panels were β = 1 ([A–E] and solid curve in [G]) or 0.5 ([F], [H], and dashed curve in [G]); <i>x</i>_f = 400 μm ([A–C] and [F–H]) or 450 μm (D–E); <i>f</i>₀ = 0 (B), 400 ([A], [C], [F–H], and solid curves in [D] and [E]), or 1,100 (dashed curves in [D] and [E]); <i>k</i>_deg = 500 sec⁻¹ ([A] and [C–G]) or 0.03 sec⁻¹ (B); <i>k</i>_max = 0.001 ([A] and [C–G]) or 4 (B); <i>V</i>(<i>x</i>) = 5 for <i>x</i> > <i>x</i>_f – 40, zero otherwise ([A], [D], [E], and solid curves in [B], [C], and [G]), 2.5 for <i>x</i> > <i>x</i>_f – 40, zero otherwise (dashed curves in [B] and [C]), 0.2 for all <i>x</i> ([F], dashed curve in [G], and solid curve in [H]), or 0.1 for all <i>x</i> (dashed curve in [H]).…”

“Knockin” Mouse Model by June Yao (23)

“…</p> <p>(D) Western blot showing markedly decreased expression of α-actinin-4 protein in K228E/K228E homozygous mice and moderately decreased expression in heterozygotes. …”

<i>ELMO1</i> promotes cell migration and invasion by RA FLS. by John W. Whitaker (728679)

“…<p>(<b>A</b>) Cell migration in the wound closure assay shows that <i>ELMO1</i> knockdown with siRNA decreases cell migration whereas the control or ELMO2 siRNAs do not. …”

TRLS2^-/- mice demonstrate appropriate hormone responses to low blood glucose. by Daniel W. Fisher (4872406)

“…<p><b>A)</b> Blood glucose was assessed at two time points in <i>Trls2</i>^+/+, <i>Trls2</i>^+/-, and <i>Trls2</i>^-/- mice, one hour after lights on (top graph) and an hour after lights off (bottom graph). …”

Table 1_Evaluation of the clinical application of information-motivation-behavior model care combined with labetalol pharmacotherapy for patients with hypertensive disorders of pre... by Linling Sun (22465585)

“…The IMB care consisted of the establishment of a dedicated nursing intervention team, informational interventions (e.g., health education), motivational interventions (e.g., personalised dietary life plan development), and behavioral interventions (e.g., guidance on self-monitoring of blood pressure).…”

Gangliosides are required for viral capture mediated by mDC. by Nuria Izquierdo-Useros (91232)

“…Data show mean values and standard error of the mean (SEM) from five independent experiments including cells from at least six donors. mDCs capture significantly higher amounts of GM3-containing LUV_HIV-tRed than Cer or Ø LUV_HIV-tRed (<i>p</i><0.0001, paired <i>t</i> test). mDCs capture significantly higher amounts of GM1a-containing LUV_HIV-tRed than negatively charged PS-LUV_HIV-tRed (<i>p</i> = 0.0081, paired <i>t</i> test). …”

Quantitative analysis <i>in vivo</i> MRI scans. by Lucas W. E. Starmans (379995)

“…<p><i>In vivo</i> T₂ of the thrombus pre- and post-injection with FibPep-ION-Micelles (n = 5) and NCFibPep-ION-Micelles (n = 4). The horizontal lines represent the group means and the average mean for the two groups combined (n = 9). …”

HepR21 cells have increased resilience to nutrient deprivation compared to HepG2 cells. by Paramita Saha (473954)

“…A set of cells were kept as control. The survivability of HepR21 cells was found to remain unaffected while HepG2 cells were highly sensitive upon prolonged starvation with an overall 50% decrease in growth after 36 h of starvation. …”

DataSheet_1_Treatment With Lipopolysaccharide Induces Distinct Changes in Metabolite Profile and Body Weight in 129Sv and Bl6 Mouse Strains.docx by Maria Piirsalu (8627697)

“…According to the general linear model analysis (GLM), the 1.5 h LPS challenge in Bl6 caused a decrease of propionylcarnitine (C3), glucogenic amino acids, and acetylornithine (Ac-Orn), whereas the response of 129Sv included decreased concentrations of short-chain acylcarnitines (SCACs), citrulline, and elevation of glycerophospholipid (PCaa C42:0) and sphingolipid [SM(OH)C16:1]. 24 h after LPS administration, robust alterations in lipid profile were observed in both strains. …”

DataSheet1_Metformin Combining PD-1 Inhibitor Enhanced Anti-Tumor Efficacy in STK11 Mutant Lung Cancer Through AXIN-1-Dependent Inhibition of STING Ubiquitination.PDF by Zhiguo Wang (219272)

“…Next, we found that metformin decreased K48-linked ubiquitination of STING and inhibited the interaction of E3-ligand RNF5 and STING. …”

Shed GP induces secretion of cytokines from DCs and MØ. by Beatriz Escudero-Pérez (663743)

“…<p>(<b>A</b>) 5×10⁵ of DCs (in white) and MØ (in gray) were incubated for 24 h with concentrated culture supernatants as in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004509#ppat-1004509-g002" target="_blank">Figure 2</a>. …”

Statistical results from mixed-effects ANOVAs for all metabolic and hormonal variables. by Amy C. Reynolds (314616)

“…A trend for decreasing testosterone was found. …”

Dataset for: Empagliflozin normalizes the size and number of mitochondria and prevents reduction in mitochondrial size after myocardial infarction in diabetic hearts. by Masashi Mizuno (5287904)

“…Tissues were sampled from the posterior ventricle (i.e., the remote non-infarcted region in rats with MI). …”

Image_1_Assessment of Epigenetic and Phenotypic Variation in Populus nigra Regenerated via Sequential Regeneration.tif by Weixi Zhang (1626823)

“…We detected a significant difference in methylation levels between first regeneration regenerated plants (G1) and those of the other four generations (G2–G5); there were no significant differences among the four later generations. …”

Data Sheet 1_Mechanical property changes of glial LC and RGC axons in response to high intraocular pressure.docx by Bochao Ma (14572143)

“…</p>Results<p>Compared to the control group, the Young's modulus of the glial LC decreased by 35.5%, 74.2%, and 80.6% at 4, 8, and 12 weeks of elevated IOP, respectively. …”

microRNA-14 as an efficient suppressor to switch off ecdysone production after ecdysis in insects by Kang He (189002)

“…<p>The precise increase and decrease of hormone ecdysone are critical for accurate development in insects. …”

Conditional ablation of <i>Tra2b</i> causes perinatal lethality and disturbed cortical patterning in mice. by Markus Storbeck (191895)

“…Cells of the ventricular and subventricular zones of the cortex show strongest decrease in staining intensity (black arrowhead). Scale bar equals 400 µm; ctx, cortex; th, thalamus; cpt, caudoputamen; cp, cortical plate; iz, intermediate zone; svz, subventricular zone; vz, ventricular zone; sn, septal nuclei; 3v, third ventricle; lv, lateral ventricle; pc, choroid plexus.…”

Assessment of SnO2 nanoparticles' impact on local Pichoclorum atomus growth performance, cell morphology and metabolites content by Bounnit, Touria

“…The current study investigates the ecotoxicity of SnO2 nanoparticles on a local marine algae isolate. Five different concentrations (1, 5, 25, 50 and 100mg/L) were tested and the culture was followed for 72h. …”
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Ligand Substitutional Effects on Molecular Dynamics and Broadband Near-White Emission in 2D 2‑Fluoroethylammonium Cadmium Chlorobromide by Maciej Ptak (1713061)

“…Optical studies showed that ligand substitution causes narrowing in bandgap energy from 5.12 to 4.28 eV and triggering of white to yellow-green emission thermally quenched above 240 K.…”