Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

The effect of HA digestion and HA replenishment alone or with CS on barrier function measured by TEER. by Charlotte J. van Ginkel (20790466)

“…In (B) and (C) each point represents a mean ± standard deviation. …”

<i>In silico</i> analysis of the effect of CDH13 variants. by Thegna Mavroconstanti (440553)

“…The reliability index (RI) for a large decrease (ΔΔG<−0.5) ranged from 0–10. For the G113R* a reliability index for a neutral stability change was given. …”

Data_Sheet_1_Age-related changes in EEG signal using triple correlation values.docx by Yuri Watanabe (19735819)

“…The subjects were 50 healthy elderly subjects (mean age 73.0 ± 5.1 years), 34 healthy younger subjects (mean age 28.1 ± 4.6 years), and 21 dementia patients (mean age 70.1 ± 9.1 years). …”

Data_Sheet_1_Stronger microbial nutrient limitations in subsoil along the precipitation gradient of agroecosystem: insights from soil enzyme activity and stoichiometry.PDF by Jingjing Yang (119262)

“…</p>Results and Discussion<p>We found that N- and P-acquiring enzymes have a tendency to decrease or increase, respectively, but C-acquiring enzymes did not change along the precipitation gradient throughout soil profiles. …”

The role of FYCO1-dependent autophagy in lens fiber cell differentiation by Shahid Y. Khan (8103215)

Inhibition of pancreatic tumor growth by NOX inhibition. by Weiqin Lu (166093)

“…<p>(A) Decrease of NOX activity by stable shRNA knockdown of p22phox (p22phox-shRNA) in Panc-1 cells, but not in cells with control shRNA (c-shRNA) knockdown. *** <i>p</i><0.001 (<i>n</i> = 3). …”

Spontaneous regeneration of lymphatics after tissue transfer can be used to bypass damaged lymphatics. by Alan Yan (298075)

“…</b> High power (40x) photomicrographs of tail skin harvested 5 mm distal to the excision site. Note decreased cellularity in skin-grafted section (left) as compared with excision section (right; arrow). …”

Datasheet1_Expected small left heart size in the presence of congenital diaphragmatic hernia: Fetal values and Z-scores for infants confirmed to have no heart disease postnatally.p... by Anita J. Moon-Grady (14223251)

“…CDH-specific models derived from log-transformed values yielded left-shifted distributions, reflecting the small structures (mean z-score lower by: MV 0.99 ± 0.30, AoV 2.04 ± 0.38, LV length 1.30 ± 0.36, and LV width 4.69 ± 0.28; p < 0.0001 for all comparisons). …”

Grip strength after forearm cooling in healthy subjects by Pedro Henrique Reis Rabelo (10438725)

“…There was also a gradual increase in grip strength during the recovery phase, with significant differences (p < 0.05) between the mean immediately after immersion and means at 5, 15 and 30 minutes after exposure to ambient temperature. …”

Ift88 facilitates polarization in migrating cells. by Christopher Boehlke (74656)

“…<b>(D)</b> Quantification of Golgi apparatus polarization (for details see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140378#sec009" target="_blank">methods</a>) showed a significant decrease of polarized Golgi in Ift88-knockdown conditions (+Tet: 37.5 ±4.8%) compared to non-induced controls (-Tet: 53.6 ±3.3%), p<0.05 (Chi²-Test), n = 3, -Tet: 258 cells/ +Tet: 269 cells. …”

Characterisation and microbial community analysis of lipid utilising microorganisms for biogas formation by Alexis Nzila (181169)

“…Acidic pH, temperature > = 45°C and NaCl > 3% led to a significant decrease in the rate of biogas formation. …”

EGF enhances cyclin G2 degradation. by Stefanie Bernaudo (4167634)

“…A dose-dependent decrease of cyclin G2 stability was also observed. …”

Table_2_The Fight Against Panax notoginseng Root-Rot Disease Using Zingiberaceae Essential Oils as Potential Weapons.pdf by Yan-Jiao Yin (5816015)

“…The decreasing trend of net photosynthetic rate (P_n), stomatal conductance (g_s), intercellular CO₂ concentration (C_i), and transpiration rate (T_r) were all alleviated. …”

Image_1_The Fight Against Panax notoginseng Root-Rot Disease Using Zingiberaceae Essential Oils as Potential Weapons.pdf by Yan-Jiao Yin (5816015)

“…The decreasing trend of net photosynthetic rate (P_n), stomatal conductance (g_s), intercellular CO₂ concentration (C_i), and transpiration rate (T_r) were all alleviated. …”

Effect of tourniquet use during total knee arthroplasty on global inflammatory cytokine changes associated with ischemia- reperfusion injury by E. Todd Schroeder (4091569)

“…Overall, both pro-inflammatory and anti-inflammatory cytokines (including tumor necrosis factor-α, interleukin 1β, 4, 5, 12, and monocyte chemtactic protein-1) were found to increase upon application of tourniquets during cementation and decrease without tourniquet use or upon application of operative tourniquets. …”

Table_1_The Fight Against Panax notoginseng Root-Rot Disease Using Zingiberaceae Essential Oils as Potential Weapons.pdf by Yan-Jiao Yin (5816015)

“…The decreasing trend of net photosynthetic rate (P_n), stomatal conductance (g_s), intercellular CO₂ concentration (C_i), and transpiration rate (T_r) were all alleviated. …”

Table_1_Renewed Attention Needed for Prevention of Sudden Unexpected Death in Infancy in the Netherlands.DOCX by Floortje Kanits (11711483)

“…However, also bed-sharing almost doubled from 5.6% in 2010/11 to 10% in 2017. Breastfeeding decreased between 1999 and 2010/11, but increased from 34% in 2010/11 to 42% in 2017. …”

I-Mutant results for selected nsSNPs in the TRIM22 protein. by Jenna N. Kelly (591069)

“…  =  number of deleterious predictions; nsSNPs with 4 or more deleterious predictions are considered high-risk nsSNPs, while nsSNPs with less than 4 deleterious predictions are considered low-risk; DDG: free energy change value in Kcal/mol (>0 increase, <0 decrease, >0.5 large increase, <−0.5 large decrease); Sign of DDG: the direction of the change (increase or decrease); The reliability index (RI) from 0–9 is shown in parentheses, where 0 is the lowest RI and 9 is the highest); PTM: predicted post-translational modification site; ConSurf results are shown in the last column (number represents the conservation score (CS) from 1–9, letter represents whether the residue was predicted to be exposed (e) or buried (b), putative functional residues are indicated with bold text; whereas putative structural residues are indicated with italicized text (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101436#pone.0101436.s001" target="_blank">Figure S1</a>); Sites with an additional ConSurf result in parentheses are located next to putative functional (9e) or structural (9b) residues; nsSNPs with the largest predicted stability decreases (DDG <−1.0) that also have a RI score of ≥5 are indicated with an asterisk.…”