AmB-resistant strains critically depend on high levels of Hsp90 function for survival. by Benjamin Matteson Vincent (477570)

“…<p>(A) Mild inhibition of Hsp90 not only reverses AmB resistance but selectively kills AmB-resistant isolates. Spot assays (5-fold serial dilutions) of wild-type (SC5314), fluconazole-resistant (Isolate #2, <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001692#pbio.1001692-White2" target="_blank">[61]</a>), and AmB-resistant (ATCC 200955) <i>C. albicans</i> on RPMI media containing antifungals and/or Hsp90 inhibitors at the indicated concentrations. …”

Portrait of Ependymoma Recurrence in Children: Biomarkers of Tumor Progression Identified by Dual-Color Microarray-Based Gene Expression Analysis by Matthieu Peyre (241074)

“…Conversely, ASPM expression was more frequently positive at relapse (87.5% vs 37.5%, p = 0.03). Loss or deletion of the MT genes cluster was never observed at relapse. …”

Excess mortality and hospitalizations in transitional-age youths with a long-term disease: A national population-based cohort study by Antoine Rachas (197007)

“…Their mortality rate between the ages of 14 and 21 years was 20.9/10,000 person-years (13.7–32.1) <i>versus</i> 1.9 (1.5–2.5) for adolescents without a LTD. Mortality was higher in males than females in youths without a LTD, but not in those with a LTD. …”

RAD21 is necessary for ERα binding within the <i>MYC</i> locus but not an exogenous ERE. A) RAD21 silencing abrogates ERα binding upstream of <i>MYC</i>. by Miranda V. McEwan (121381)

“…MCF7 cells were transfected for 48 hours with Control, RAD21 or ESR1 siRNA and either a negative control (NEG) plasmid, a positive control plasmid (POS) or a plasmid encoding the firefly luciferase reporter gene under the control of 5 EREs. Cells were treated with vehicle (V) or estradiol (E) for 24 hours before luciferase activity was measured. …”

A robust intracellular metabolite extraction protocol for human neutrophil metabolic profiling by Susama Chokesuwattanaskul (5813888)

“…Cell number and number of scans (NS) were optimised as >3.6 million cells and 512 NS. 327 spectral bins were defined in the neutrophil spectra, of which 287 (87.7%) were assigned to 110 metabolites that included: amino acids, peptides and analogues; carbohydrates, carbonyls and alcohols; nucleotides, nucleosides and analogues; lipids and lipid-like molecules; benzenoids; and other organic compounds. 43 metabolites changed at least 1.5 fold (increase or decrease) after the addition of PMA for 5 or 15 min. …”

2′-<em>O</em> Methylation of the Viral mRNA Cap by West Nile Virus Evades Ifit1-Dependent and -Independent Mechanisms of Host Restriction In Vivo by Kristy J. Szretter (165885)

“…In comparison, WNV-E218A was virulent after intracranial infection as judged by increased infection in different regions of the central nervous system (CNS) and a greater than 16,000-fold decrease in LD₅₀ values in <em>Ifit1</em>^−/− compared to wild type mice. …”

Assessing Mitochondrial DNA Variation and Copy Number in Lymphocytes of ~2,000 Sardinians Using Tailored Sequencing Analysis Tools by Jun Ding (80820)

“…Both homoplasmies and heteroplasmies show 5-fold higher transition/transversion ratios than variants in nuclear DNA. …”

Table1_In utero arsenic exposure and early childhood motor development in the New Hampshire Birth Cohort Study.docx by Erin E. Butler (15428729)

“…In models adjusted for potential confounders, each doubling of urine tAs decreased overall motor proficiency by –3.3 points (95% CI: –6.1, –0.4) for tAs concentrations greater than the change point of 9.5 µg/L and decreased fine motor integration by –4.3 points (95% CI: –8.0, –0.6) for tAs concentrations greater than the change point of 17.0 µg/L.…”

Image1_In utero arsenic exposure and early childhood motor development in the New Hampshire Birth Cohort Study.tiff by Erin E. Butler (15428729)

“…In models adjusted for potential confounders, each doubling of urine tAs decreased overall motor proficiency by –3.3 points (95% CI: –6.1, –0.4) for tAs concentrations greater than the change point of 9.5 µg/L and decreased fine motor integration by –4.3 points (95% CI: –8.0, –0.6) for tAs concentrations greater than the change point of 17.0 µg/L.…”

K_eq and Φ of the α subunit. by Pallavi A. Bafna (271514)

“…Φ-values of 55 different residues plotted as a function of sequence position (≥2 mutants and >5-fold range in K_eq). Subunit domains are shown along the x-axis. …”

Image_1_Increased miR-142-3p Expression Might Explain Reduced Regulatory T Cell Function in Granulomatosis With Polyangiitis.JPEG by Gerjan J. Dekkema (7375049)

“…Overexpression of miR-142-3p significantly decreased cAMP levels (p = 0.02) and tended to decrease the mRNA levels of a predicted target gene, adenylate cyclase 9 (ADCY9; p = 0.06). …”

Table_1_Increased miR-142-3p Expression Might Explain Reduced Regulatory T Cell Function in Granulomatosis With Polyangiitis.pdf by Gerjan J. Dekkema (7375049)

“…Overexpression of miR-142-3p significantly decreased cAMP levels (p = 0.02) and tended to decrease the mRNA levels of a predicted target gene, adenylate cyclase 9 (ADCY9; p = 0.06). …”

Data_Sheet_1_Cerebrospinal fluid micro-volume changes inside the spinal space affect intracranial pressure in different body positions of animals and phantom.PDF by Marijan Klarica (584483)

“…When a fluid volume (CSF or artificial CSF) was removed from the spinal space, both lumbar and cranial CSFP pressures decreased by 2.0–2.5 cm H₂O for every extracted 100 μL. …”

G protein involvement in US28-mediated Tcf-Lef activation. by Ellen V. Langemeijer (120625)

“…Tcf-Lef reporter gene activation was measured 24 hr after transfection and is displayed here as the percentage of the mock control that is set at 100%. B, HEK293T cells were co-transfected with the Tcf-Lef reporter gene construct, US28-expressing construct or empty plasmid control (mock) and an shRNA construct to decrease protein levels of Gα_q. …”

Hemodialysis-Related Glycemic Disarray Proven by Continuous Glucose Monitoring: Glycemic Markers and Hypoglycemia by Akinori Hayashi (305316)

“…However, the exact glycemic fluctuation profiles and frequency of asymptomatic hypoglycemia using dialysates containing >100 mg/dL glucose have not been determined.</p> <p><b>RESEARCH DESIGN AND METHODS</b></p> <p>We evaluated the glycemic profiles of 98 type 2 diabetes patients undergoing HD (68 men, HbA1c 6.4±1.2%, glycated albumin 20.8±6.8%) with a dialysate containing 100, 125, or 150 mg/dL glucose using continuous glucose monitoring.…”

Differential OTU abundance of anode-attached microbes and reactor microbes between M1L and M2H reactors. by Nattawet Sriwichai (18847466)

“…<p><b>A</b>, Volcano plot demonstrating significantly different OTUs between M1L and M2H using the criteria: a log-transformed FDR < 0.05 and an absolute log₂ fold-change ≥ 5. 74 OTUs are downregulated while 43 OTUs are upregulated in M1L/M2H. …”

Analysis of vesicle recruitment to the RRP for models covered by Eq 1. by Kashif Mahfooz (2586439)

“…The exponential constant for acceleration (<i>τ</i> ⋅ <i>ν</i> in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1004855#pcbi.1004855.e026" target="_blank">Eq 2</a>) was 10 action potentials for 100<i>Hz</i> and 300<i>Hz</i> stimulation alike, and fatigue was modeled as a 10%/<i>s</i> linear decrease. …”

7-Valent Pneumococcal Conjugate Vaccination in England and Wales: Is It Still Beneficial Despite High Levels of Serotype Replacement? by Yoon Hong Choi (52781)

“…</p> <h3>Conclusion</h3><p>Our model suggests that PCV7 vaccination could result in a decrease in overall invasive pneumococcal disease, particularly in children, even in an environment of rapid replacement with non-PCV7 serotypes within 5 years of vaccine introduction at high coverage.…”

Loss of PLZF Expression in Prostate Cancer by Immunohistochemistry Correlates with Tumor Aggressiveness and Metastasis by Guang-Qian Xiao (711983)

“…<div><p>PLZF is a transcription repressor, which plays a critical role in development, spermatogenesis and oncogenesis. …”

Easy generation of highly responsive cell lines with the all-in-one doxycline-controlable lentiviral vector CEST. by Karim Benabdellah (158724)

“…The leaking in the absence of doxycycline decrease as the vector copy number increase (left graphs from top to bottom) <b>C</b>) Fold induction (left panels) and leaking (right panels) in 293 T (top panels) and human mesenchymal stem cells (hMSC) (bottom panels) transduced with increasing MOIs of the CEST vector. …”