Heat map analysis of selected peptides. by Sayani Dasgupta (104387)

“…Bright red indicates peptides that were elevated ≥100% (ratio ≥2.00). The three groups in the heat map indicated by lines and numbers on the left margin indicate peptides which showed a large or partial decrease (set 1), little or no change (set 2), or a large increase (set 3) in response to the proteasome inhibitors. …”

Partial unwinding of backbone modified DNA substrates by BACH1-WT helicase by Rigu Gupta (22923)

“…As the dsDNA is unwound, the dye molecules are displaced (open ovals) which is measured by a decrease in the amount of fluorescence. In Scheme 1, the unmodified DNA substrate is fully unwound by the helicase, resulting in a maximal change in fluorescence. …”

Gangliosides are required for viral capture mediated by mDC. by Nuria Izquierdo-Useros (91232)

“…(B) Comparative mDC capture of VLP_HIV-Gag-eGFP produced in HEK-293T and distinct fluorescent LUV_HIV-tRed containing Cer, GM3, GM2, GM1a, or PS. A total of 2×10⁵ DCs were pulsed for 4 h at 37°C with 100 µM of LUV or 75 ng of VLP_HIV-Gag-eGFP Gag in 0.2 ml, washed with PBS, and assessed by FACS to obtain the percentage of tRed or eGFP-positive cells. …”

Fig 11 - by Maike Stegen (8578665)

“…Actin β at approx. 40 kDa (molecular weight 43 kDa) was used as a reference on a stripped membrane. Stimulation with 100 nM PMA led to a distinct decrease in GRK6 protein expression. …”

Penile cancer trends and economic burden in the Brazilian public health system by Fernando Korkes (5910167)

“…Conclusion: Despite the decrease in admissions, penile cancer still imposes a significant economic and social burden to the Brazilian population and the Public Health System.…”

cAMP dynamics in adult mouse ventricular Epac1-camps expressing cardiomyocytes upon treatment with cAMP elevating agents and PDE4 inhibition. by Julia U. Sprenger (3387719)

“…<b>(C)</b> Representative FRET traces from Epac1-camps cardiomyocytes stimulated with the β-AR agonist isoproterenol (ISO, 100 nM) or <b>(E)</b> with the direct AC activator forskolin (10 μM) leading to an increase of cAMP visualized as a decrease in the FRET ratio. …”

Changes in <i>PTGS1</i> and <i>ALOX12</i> Gene Expression in Peripheral Blood Mononuclear Cells Are Associated with Changes in Arachidonic Acid, Oxylipins, and Oxylipin/Fatty Acid... by Claire C. Berthelot (838064)

“…</p><p>Methods</p><p>Plasma FA and oxylipin profiles from 12 healthy individuals were compared to PBMC gene expression profiles following six weeks of supplementation with fish oil, which delivered 1.9 g/d eicosapentaenoic acid (EPA) and 1.5 g/d docosahexaenoic acid (DHA). Fold changes in gene expression were measured by a quantitative polymerase chain reaction (qPCR).…”

Differentiation study, NOTCH and WNT expression changes in REX1 knocked-down hUCB-MSCs. by Dilli Ram Bhandari (249345)

“…(D) The expression levels of <i>FZD2</i>, <i>LRP5</i> and <i>DKK1</i> decreased after REX1 knockdown in hUCB-MSCs. …”

Tie-2 Knock-Down Inhibits MLC-p and Promotes Disrupted Endothelial Architecture, Replicating the Effects of Ang-2 by Samir M Parikh (72439)

“…<div><p>(A) HMVECs were stimulated with Ang-2 (100 ng/ml) in 2.5% FBS EBM-2 for the indicated times, and phospho-Tie-2 was detected by immunoprecipitation and Western blot (upper) as described in Methods. …”

Validation of the primary screen hits with secondary luciferase-based invasion and red-green invasion assays. by Rajshekhar Y. Gaji (417441)

“…The values obtained with non-targeting siRNA controls were set at 100% invasion. A total of 81 genes (red asterisks) showed significant decrease (<i>p</i><0.05, one sample <i>t</i>-test) in parasite invasion compared to NTsiRNA controls. …”

Expression of MMP7 and TIMP1 in wounds. by Katharina Sommer (463202)

“…Septic animals show a significant decrease in MMP7 expression on day 2 and 6 compared to control mice. …”

Ca²⁺ sparklets in DSM are affected by, but not dependent upon, signals from the SR. by Peter Sidaway (190696)

“…In the presence of the VGCC antagonist R-(+)-Bay K 8644 (1 μM) and CPA (10 μM), there was a significant decrease in the frequency (D) but not amplitude (E) of Ca²⁺ sparklets. …”

Glycosylation inhibitors block thrombin-induced sRBC adhesion to HUVECs. by Pushpankur Ghoshal (580530)

“…The level of sRBC adhesion to untreated HUVECs was set to 100%. **, p<0.0001 <b>B</b>. Glycosylation inhibitors decrease thrombin-induced sRBC adhesion to HUVECs. …”

Astrocytic volume changes in the cortex of GFAP/EGFP and GFAP/EGFP/α-Syn^−/− mice during hypoosmotic stress. by Miroslava Anderova (155561)

“…The changes of astrocytic volume during washout were evaluated in each individual cell and expressed as an average percent of cell volume increase/decrease related to the maximal volume after 30 minutes of aCSF_H-50 (<b>C right</b>) or aCSF_H-100 (<b>D right</b>) exposure; these values were set as 0%. …”

Hormone addition results in chromatin changes at individual GR-regulated genes. by Chuong D. Pham (111374)

“…We calculate that a nucleosome/bare ratio of 1.0 corresponds to ∼50% nucleosome occupancy, while a value of 2.1 corresponds to 100% occupancy (e.g. a nucleosome covering a given location on all gene copies). …”

Change in the number of attractors during the learning process. by Tomoki Kurikawa (228085)

“…In the early stages of the learning process, the number of fixed-point attractors increases, while in the later stage (after 5 or 6 learning steps), the number of fixed-point attractors decreases (fixed-point attractors are replaced by limit-cycle attractors).…”

Histology of islet allografts at day 10, 17, 24, and 31 after transplantation in non-diabetic mice. by Jyuhn-Huarng Juang (407970)

“…There was profound immune cell infiltration at day 10 and 17, and a decrease in graft size at day 24 and 31 in the CSPIO-labeled islet graft. …”

Gene expression in primary human skeletal muscle cells after treatment with acylcarnitines. by Rainer Lehmann (141280)

“…<p>mRNA expression of PGC-1α (A), CPT1b (B), CD36 (C), COX1 (D) and AngPL-4 (E) related to β-actin mRNA levels after incubation with 100 µM L-carnitine (L-C), an equimolar mixture of C8:0-, C10:0- and C12:0-acylcarnitine (total 100 µM; AC), 60 µM palmitate (p), or p and AC as indicated. …”

Effect of galactose treatment on oleic acid metabolism. by Eili Tranheim Kase (177084)

“…Suppressibility, defined as the ability of the cells to decrease oleic acid oxidation by acutely added glucose, was calculated as: [(1-(oxidation of oleic acid at 5 mM glucose/oxidation of oleic acid at no glucose added))×100%]. …”

Design, synthesis and biological evaluation of marine naphthoquinone-naphthol derivatives as potential anticancer agents by Yujuan Li (554062)

“…Among these, Compound <b>13</b>, featuring an oxopropyl group at the <i>ortho</i>-position of quinone group, exhibited the most potent inhibitory effects on HCT116, PC9, and A549 cells, with IC₅₀ values decreasing from 5.27 to 1.18 μM (4.5-fold increase), 6.98 to 0.57 μM (12-fold increase), and 5.88 to 2.25 μM (2.6-fold increase), respectively, compared to compound <b>5</b>. …”