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Decrease of neuronal FKBP4/FKBP52 modulates perinuclear lysosomal positioning and MAPT/Tau behavior during MAPT/Tau-induced proteotoxic stress
Published 2021“…</p> <p><b>Abbreviations:</b> AD: Alzheimer disease; AKT/protein kinase B: AKT serine/threonine kinase; ALP: Autophagy-lysosomal pathway; ATG: autophagy-related; BafA<sub>1</sub>: bafilomycin A<sub>1</sub>; CQ: chloroquine; CTSD: cathepsin D; DIV: days in vitro; DRG: dorsal root ganglion neurons; Dox: doxycycline; DNAJC5: DnaJ heat shock protein family (Hsp40) member C5; EL: empty lentiviral vectors; ENO2/NSE: enolase 2, gamma neuronal; FKBP4/FKBP52: FKBP prolyl isomerase 4; FTLD-Tau: frontotemporal lobar degeneration with Tau pathology; GFP: green fluorescent protein; LAMP1: lysosomal associated membrane protein 1; LDH: lactate dehydrogenase; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; MAPT/Tau: microtubule associated protein tau; MTT: tetrazolium salt; NFTs: neurofibrillary tangles; RPE-1: retinal pigment epithelial cells; shRNA: small-hairpin ribonucleic acid; SQSTM1/p62: sequestosome 1; SD: standard deviation; SEM: standard error of the mean; SH-SY5Y: human neuroblastoma cells; Sh1 or Sh2: Lentiviral shRNA vectors inducing FKBP4 decrease; SH-52GFP: MAPT/Tau-inducible SH-SY5Y cell line constitutively expressing FKBP4-GFP; TUBB3/βIII tubulin: tubulin beta 3 class III; UPS: ubiquitin-proteasome system</p>…”
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Hydrogen peroxide scavenging by the <i>Aedes aegypti</i> peritrophic matrix.
Published 2023Subjects: -
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GSK343 decreased neuroblastoma viability, proliferation, and motility.
Published 2021“…<p>(A) SK-N-AS, SK-N-BE(2), SH-EP and WAC(2) cells (1.5 × 10<sup>3</sup> cells) were treated with increasing concentrations of GSK343 (0, 5, 15, 25 μM) for 24 hours and viability was measured using alamarBlue<sup>®</sup> assay. …”
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R346K Mutation in the <i>Mu</i> Variant of SARS-CoV‑2 Alters the Interactions with Monoclonal Antibodies from Class 2: A Free Energy Perturbation Study
Published 2022“…For comparison, the addition of the K417N mutation, present in the newly registered <i>Mu</i> variant in July 2021 in the U.K., affected the class 1 mAbs by strongly reducing the binding by 1.29 kcal/mol or about 10-fold. Thus, the resistance effect of the R346K mutation on the <i>Mu</i> variant is possible but not so significant and is due to the additional decrease of antibody neutralization based on the reduced binding of class 2 antibodies.…”