ROP18 directly phosphorylates Irga6 at T102 and T108 in vitro. by Tobias Steinfeldt (154465)

“…<p>(A) Bacterially expressed, purified wt or T102A and T108A mutant Irga6 proteins (300 ng) were coincubated with bacterially expressed, purified GST-ROP18-Ty (10 µl, 5 µl, 1.7 µl respectively; 1 µl ∼20 ng) in the presence of γ³²P-ATP in vitro. …”

Synaptotoxic PrP^Sc species are propagated from early in the evolution of M1000 prion disease. by Simote Totauhelotu Foliaki (5614364)

“…The HFS trains were applied following 30 minutes of baseline recordings. (A, C, D) Results are presented as mean ± standard error of mean. …”

Large-scale analysis by SAGE reveals new mechanisms of oncogene action-2 by Corinne Bresson (73910)

“…The grey bars represent a mean of ratio calculated using the three reference genes , and . …”

Negative feedback modulates the visual response gain but not the feature selectivity. by Anastasiia Vlasiuk (11172471)

“…A sigmoid function (Eq (6) in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0254611#sec009" target="_blank">Methods</a>) was fitted to quantify the nonlinear profile (e.g., light blue for the suppressed period at 0–100 ms after the nerve shock, with the upper and lower bounds of the cell’s firing probability in dotted horizontal lines). …”

TGF-β1 signaling regulates steroidogenic gene expression, affecting testicular testosterone levels in mice. by Eunsook Park (299902)

“…<p>(<b>A</b>) Decreased Tgfbr2^fl°^x allele in purified primary Leydig cells isolated from mice harboring the Cyp17iCre transgene. …”

Efficacy and selectivity of the shSNP <i>in vitro</i>. by Valérie Drouet (3179907)

“…Levels of the chimeric <i>HTT</i> mRNA were normalized to β-Actin and are reported as mean percentages relative to the control condition (set at 100%) ± SEM (n = 3–5). …”

Chordoma preclinical models are insensitive to BAF complex inhibitor FHD-286, alone or in combination with gemcitabine by Chordoma Foundation (4391989)

“…A panel of 4 chordoma cell lines were seeded in a white-walled 96-well plate so that at the start of treatment, 36 hours after plating, the confluence was 15%. …”

Self-limiting of the gTLR9 signaling by <i>gTlr9</i> alternative splicing. by Frank Fang-Yao Lee (2210863)

“…DRB pre-treatment (0 h) led to slight increase of <i>9A</i> (<b>B</b>) and decrease of <i>9B</i> (<b>C</b>) (Cf. the two gray bars vs. white and black bars). …”

Inhibition of TOR signaling prevents meiotic onset in <i>Oikopleura dioica</i>. by Gunasekaran Subramaniam (546277)

“…There was a decrease in IdU incorporation in germline nuclei over time (D3–D18; day 3 to day 18). …”

Feijoa F3 does not activate autophagy or inhibit NF-κB activity in autophagy defective HCT116 cells. by Noha Ahmed Nasef (760610)

“…A percentage of greater than 100 shows increased inflammatory response in the presence of the ligand when compared to media (no treatment); and a percentage of less than 100 suggests a decrease in the inflammatory response in the presence of the ligand when compared to media (no treatment) meaning that a condition has inhibited NF-κB signaling. …”

The effector functions of CHK-152 contribute to protection <i>in vivo</i>. by Pankaj Pal (404355)

“…Comparison of binding of ch-CHK-152 and ch-CHK-152 N297Q to FcγR (CD16A, 500 nM; CD32A, 100 nM; and CD64, 100 nM) or C1q (50 nM), as measured by surface plasmon resonance. …”

Selective loss of macrophage subpopulations in lymphoid tissue of <i>Csf1rko</i> rats. by Dylan Carter-Cusack (20624041)

“…Positive logFC indicates an increase in expression in <i>Csf1rko</i> animals, while negative logFC indicates a decrease in expression in <i>Csf1rko</i> animals. …”

Effect of 3BrP on morphology, cell number, viability, proliferation and pluripotency. by Ana Sofia Rodrigues (491559)

“…Cells stained green were counted as live, while PI positive cells were counted as dead. 100 cells were counted per condition for a total of n = 8 independent experiments, results are expressed as means ± SEM for 30 experiments. …”

EFFECTS OF ADAPTED CANOEING ON THE CARDIOPULMONARY SYSTEM OF PARAPLEGICS by Elizângela Márcia de Carvalho Abreu (6147902)

“…The HF indices (un) and LF/HF showed no significant decrease of 5.7 and 7.0%, respectively. The highest breathing gains were for MVV (9.7%), MIP (8.5%) and MEP (11.0%), being however not significant. …”

Deletion of RBPJK enhances cell spontaneous differentiation in regular MSC culture. by Xifu Shang (784529)

“…<p>Mesenchymal stem cells were infected with Control shRNA(Co) or RBPJK shRNA (shRBPJK) lentivirus before being harvested for alkaline phosphatase (ALP) staining, western blot, RT-PCR analysis and BrdU ELISA. (A) Western Blot showed a significant decrease of RBPJK protein expression at day 3 and 14 following shRBPJK lentiviral infection. …”

Requirement of NF-κB activation for cytokine-mediated reductions in TEER. by Paul R. Clark (714064)

“…Conditions are lane 1, DMSO Control; lanes, 2, 3, 4 and 5, Bay11 at 0.375, 0.75, 1.5 and 3.0 μM, respectively all after 0.8 ng/ml TNF for 16 hours. …”

Monoamine content in operationally matched 6-OHDA lesioned rats at >70% TH protein loss. by Tanya Chotibut (292370)

“…As shown in the middle panel, there was no loss of NE in the 6-OHDA lesion method employed, but there was a trend toward a decrease in 5-HT (bottom panel). Statistics: DA, ***<i>p</i><0.0001, t = 10.87. …”

Myelin and myelin proteins are marginally reduced in the brain of <i>Syn1Cre; Galc flox/−</i>. by Conlan Kreher (13001753)

“…Scale bar = 100 μm. (<b>E</b>) Quantification of PLP level, measured by averaged integrated signal intensity, reveals a trend of reduction in the mutant brain. …”

Figure 4, supplementary figure 6 by Kristen Pleil (11084931)

“…</b>a) Proportion of responder categories during 10-minute E2 wash on across E2 doses (0.01, 1, 10, 100, 1000 nM) for frequency (top) and amplitude (bottom). b) Raw average frequency (top) and amplitude (bottom) during baseline, E2 wash on, and washout for each cell. c) Maximum % change from baseline for cells categorized as increased and decreased during E2 wash on for frequency (top) and amplitude (bottom). …”

Sensitivity of the newly designed primers. by Chao-Jen Wang (8967944)

“…<p>Agarose gel showing the sensitivity of polymerase chain reaction (PCR) using the genomic DNA of a human opportunistic <i>Fusarium oxysporum</i> (HOFo) and the primer pair Primer 5/ST33-R: Amplification of a decreasing amount of the HOFo isolate MCC2074 DNA ranging from 100 to 10⁻³ ng. …”