Supplementary Material for: One-Year Mortality Rates in US Children with End-Stage Renal Disease by Chavers B.M. (3631768)

“…One-year mortality rates showed evidence of a decrease in the number of peritoneal dialysis patients (6.03 per 100 patient-years, 1995; 2.43, 2010; p = 0.0263). …”

Formation of multiple bumps. by Yasser Roudi (105094)

“…(D), (E), and (F) are the same quantities as (A), (B) and (C) but 100 time steps after the presentation of the second pattern.…”

Supplementary Material for: Human Umbilical Cord Blood Therapy Protects Cerebral White Matter from Systemic LPS Exposure in Preterm Fetal Sheep by Paton M.C.B. (5713817)

“…<b><i>Methods:</i></b> Chronically instrumented fetal sheep at 0.65 gestation were administered lipopolysaccharide (LPS, 150 ng, 055:B5) intravenously over 3 consecutive days, followed by 100 million human UCB mononuclear cells 6 h after the final LPS dose. …”

Hv1 increases colorectal cell migration and invasion. by Yifan Wang (380120)

“…<p>A, B and C, migration kinetics of SW620 (A), SW480 (B) and HT29 (C) assayed by wounded monolayer model. nc, cells transfected with the negative control (a, b, c, d and e in left panels); si, cells transfected with siRNA (f, g, h, i and j in left panels); and Zn²⁺, cells cultured at a final concentration of 100 µM ZnCl₂ (k, l, m, n and o in left panels). …”

Ala⁴⁸²-Ser⁴⁸³ was the key peptide bond that allowed the PP-RGD domain to become inactive. by Shigeki Suzuki (661339)

“…<p>(A) MG63 cells were preincubated with closed RGD, closed 7-RGD-7, closed 5-RGD-5, closed 3-RGD-3, and 1-RGD-1 peptides (1 mM) for 10 min, and were then seeded onto vitronectin (100 nM) in serum-free medium. …”

Hv1 regulates colorectal cancer cell intracellular pH. by Yifan Wang (380120)

“…Suppression of Hv1 expression by siRNA and activity by 100 µM ZnCl₂ notably induces a decrease in intracellular pH in the highly metastatic SW620 cells.…”

Crude glycerin for beef cattle at pasture in rainy season: productive and economic viability by Leandro Munhoz SOCREPPA (6119900)

“…<p></p><p></p><p>The effect of replacing corn by crude glycerin (GB) on the performance and economic viability of beef cattle at pasture in the rainy season was evaluated. A total of 30 animals with average body weight (BW) of 238.6 ± 21.24 kg in a completely randomized design between treatments: mineral supplement and concentrate supplementation (1.0 kg/animal, 30% CP) with 0, 33, 66 and 100% of GB replacing corn. …”

ET-1 upregulated MMP-1 and MMP-13 expression and downregulated TIMP-1 release. by Wei Yuan (64653)

“…<p>Confluent CECs in six-well plates were cultured in serum-free medium for 12 hours and then treated with ET-1 (0–100 nM) for 36 hours. ET-1 increased the release of MMP-1 and MMP-13, but decreased the production of TIMP-1 in a dose-dependent pattern as shown by ELISA assay. …”

Dose response experiments. by Piotr Ruchala (197975)

“…<p>OxP-11 and OxP-13, the two most active oxpholipins in the monocyte chemotaxis assay were re-tested at three concentrations: 1 µg/ml, 0.1 µg/ml and 0.01 µg/ml. A 10-fold reduction, from 1.0 to 0.1 µg/ml caused a relatively small decrease in activity, but a 100-fold reduction to 10 ng/ml reduced activity substantially.…”

Different colorimetric profiles of ESKAPE pathogens in BacChips. by Jack Y. Ho (313569)

“…<p>We loaded devices using a single isolated colony suspended in 100 µL of loading media consisting of 25% LB and 0.05% phenol red in saline. …”

Mutations at T16 in IBV E support VLP production. by Travis R. Ruch (166896)

“…<p>(A) Immunoblot showing the amount of IBV N, M, and E in cells and released as VLPs. (10% of cell fraction, 100% of VLP fraction) (B) Quantification of immunoblot data showing the amount of M released with no E, IBV E, T16A, T16S, T16Q or T16N. …”

Endocytotic function of MECs is impacted by elevated pressure. by Xiaorong Xin (220876)

“…<p>Ben-Men-I cells (A) or PMECs (B) were treated with elevated pressure for two days and fluorescent latex beads were added to assess endocytotic activity by fluorescence microscopy (>100 cells scored/condition). …”

Changes in various parameters in various organs after burn injury. by Bartosz Szczesny (16538)

“…<p>A single arrow shows a statistically significant change; double arrows show statistically significant changes that are more than 100% increases (i.e. doubling of the parameter) or a more than 50% decrease in the given parameter. …”

60:40 and 30:70 model output. by Rachael Miller Neilan (10678614)

“…<p>The 60(PKC<i>δ</i>):40(SOM) model (<b>A</b>) and 30:70 model (<b>B</b>) were each simulated 100 times using constant 120 pA current and the average, minimum, and maximum pain output was plotted. …”

Outline of new 3D reconstruction method that uses SA algorithm. by Toshihiko Ogura (131016)

“…This procedure is iterated 100,000 times, and when finished, the temperature, the kernel size and its value-shifting SD decrease exponentially. …”

Robot contact detection. by Sean R. Anderson (308847)

“…<p>Novelty detection algorithms applied to periodic signals recorded from the whisking robot for a 100 s data record (left) and an illustrative section zoomed on the time axis (right). …”

Progressive epithelial dysfunction and aberrant follicular morphogenesis in knockout mice. by Aniruddha Sengupta (47398)

“…<p>(A–P) Back skin sections from control (A, C, E, G, I, K, M, O) and knockout mice (B, D, F, H, J, L, N) were stained with H&E for histological examination. …”

Expression of CNNMs suppress expression of EGFP. by Zhiyong Bai (259036)

“…<i>n</i> = 100. * indicates a <i>p</i>-value of less than 0.05. …”

Fis1ΔTM membrane binding requires negatively charged lipids. by Robert C. Wells (348960)

“…Each panel depicted as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0021384#pone-0021384-g001" target="_blank">Figure 1</a> with experiments done in a similar manner with 5 µM protein incubated for 4 hours at 25°C with 2 mM of 100 nm lipid vesicles (1∶400 protein∶lipid). …”

Phloroglucinol attenuates the 6-OHDA-mediated loss of antioxidant enzymes in SH-SY5Y cells and rat brains. by Junghwa Ryu (448435)

“…<p>(<b>A</b>) 50 µg of protein was added to 50 mM phosphate buffer (pH 7) containing 100 mM H₂O₂. …”