Validation of target gene knockdown at mRNA and protein levels. by Rajshekhar Y. Gaji (417441)

“…<p>A. The percent decrease in mRNA levels of target genes transfected with gene-specific siRNA were determined using q-RT PCR 72 h post-transfection. …”

Immmocytochemistry of DMT1−IRE, DMT1+IRE, Fpn1 and TfR1 proteins in cortical neurons. by Fang Du (232423)

“…<p>Cells were treated with L-DOPA (0, 1, 5, 10, 100, 200 µM) in DMEM+5%FBS for 16 hours and then immunostained for DMT1−IRE (A), DMT1+IRE (B), Fpn1 (C) or TfR1 (D) antibodies. …”

Loss of <i>foxo3b</i> function results in anterior defects. by Xun-wei Xie (206564)

“…(B1–B2, B7) The expression of <i>pax6</i> at the telencephalon and eyes was reduced in <i>foxo3b</i>-SP-MO injected embryos. (B3–B4, B8) <i>Tbx5</i> expression at the eyes decreased in <i>foxo3b</i>-knockdown embryos. …”

Data_Sheet_1_A Pilot Assessment on the Role of Procalcitonin Dynamic Monitoring in the Early Diagnosis of Infection Post Cardiac Surgery.PDF by Qiang Miao (497115)

“…In adult patients, the AUC of PCT for diagnosis of infection on PODs 1, 3, and 5 were 0.626, 0.817, and 0.806, with the optimal cut-off values of 7.35, 3.63, and 1.73 ng/ml, respectively. …”

Image 2_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Table 1_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Image 1_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Table 2_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Table_1_Efficacy and Safety of Tacrolimus Therapy for a Single Chinese Cohort With Very-Late-Onset Myasthenia Gravis.DOCX by Yiming Zheng (3921866)

“…Regarding the 13 patients, coadministering Wuzhi capsules the tacrolimus concentration increased from 2.75 (1.4–3.8) ng/ml to 5.95 (5.1–7.0) ng/ml (p = 0.012). No significant differences in outcomes were observed between tacrolimus treatment without and with corticosteroids or between the Tac group and the control group. …”

Fitness and distribution of cell types. by Joshua R. Christie (725052)

“…The decrease in heteroplasmy in <i>B</i>₁<i>B</i>₂ cells between generations 0–100 is an artifact of introducing <i>U</i>₁ at a frequency of 0.01 (the influx of <i>U</i>₁ gametes homoplasmic for the wild type haplotype converts some heteroplasmic <i>B</i>₁ and <i>B</i>₂ gametes into homoplasmic gametes, which increases the proportion of homoplasmic <i>B</i>₁<i>B</i>₂ cells). …”

datasheet1_Unraveling the Contribution of Fluid Therapy to the Development of Augmented Renal Clearance in a Piglet Model.pdf by Laura Dhondt (10032890)

“…Clearances of iohexol and amikacin increased with on average 15 and 14%, although large interindividual variability was observed. This led to decreased systemic exposure to amikacin, which was manifested as decrease in area under the plasma concentration-time curve from time 0 h to infinity from 34,807 to 30,804 ng.h/mL. …”

Fibronectin normalizes the responsiveness of cKO cells. by Nina Kawelke (194831)

“…Stellate cells were cultured directly after isolation for 24 hours on plates left uncoated or coated with fibronectin, media were changed and collected after an additional 24 hours in culture (using 10% fibronectin-depleted FCS). N = 5 wells/group. (<b>B</b>) Fibronectin coating is associated with a decrease in TGF-β-receptor-II mRNA expression in response to TGF-β stimulation in Mx-cKO cells. …”

Supplementary Material for: Flavonoid Quercetin Enhances Nuclear factor erythroid 2-related factor 2 (Nrf2) Pathway Activation and Reduces Inflammatory Cytokines in Asthmatic Airwa... by figshare admin karger (2628495)

“…Following a washout with phosphate-buffered saline, cells were exposed to IL-13 (10 ng/mL) for 3 hours. Cell culture supernatants were collected, and a cytokine panel was measured. …”

Analysis of FV-specific CD8⁺ T cell activation induced by targeting viral Ag to CD11c on DC. by Asim Ejaz (132732)

“…Data represent mean±SD of 5 independent experiments. Data were analyzed with unpaired <i>t</i>-test. …”

MiR-150 expression promotes myeloid differentiation of primary BC CML patient cells. by Valerie A. Morris (462679)

“…(B) BC CML progenitor cells from patient 1 were sorted and plated in triplicate 5 and 7 days after transduction in Methocult™ containing 20% lot-tested FBS, 10% BSA, and cytokines stated above. …”

Data Sheet 1_Development of an LC-MS/MS method for astaxanthin quantification in shrimp tissues and its application to detect astaxanthin variations during ovary development stages... by Shuo Diao (1967698)

“…The initial mobile phase composition was 0.1% formic acid in 3 mM ammonium acetate in water (solvent A) and methanol (solvent B) at a ratio of 15:85 (v/v), with a flow rate of 0.20 mL/min.</p>Results<p>The assay demonstrated linearity over a concentration range of 20 to 10,000 ng/mL, with accuracy varying from −0.1 to 1.7% and precision within 2.1%. …”

Image_1_Effects of acromegaly treatment on left ventricular systolic function assessed by speckle tracking echocardiography in relation to sex differences: results from a prospecti... by Agata Popielarz-Grygalewicz (15424142)

“…</p>Results<p>Treatment with SRL resulted in reduction in median (IQR) GH&IGF-1 levels after 3 months, from 9.1(3.2-21.9) to 1.8(0.9-5.2) ng/mL (p<0.001) and from 3.2(2.3-4.3) to 1.5(1.1-2.5) xULN (p<0.001), respectively. …”

Image_3_Effects of acromegaly treatment on left ventricular systolic function assessed by speckle tracking echocardiography in relation to sex differences: results from a prospecti... by Agata Popielarz-Grygalewicz (15424142)

“…</p>Results<p>Treatment with SRL resulted in reduction in median (IQR) GH&IGF-1 levels after 3 months, from 9.1(3.2-21.9) to 1.8(0.9-5.2) ng/mL (p<0.001) and from 3.2(2.3-4.3) to 1.5(1.1-2.5) xULN (p<0.001), respectively. …”

Image_4_Effects of acromegaly treatment on left ventricular systolic function assessed by speckle tracking echocardiography in relation to sex differences: results from a prospecti... by Agata Popielarz-Grygalewicz (15424142)

“…</p>Results<p>Treatment with SRL resulted in reduction in median (IQR) GH&IGF-1 levels after 3 months, from 9.1(3.2-21.9) to 1.8(0.9-5.2) ng/mL (p<0.001) and from 3.2(2.3-4.3) to 1.5(1.1-2.5) xULN (p<0.001), respectively. …”

Image_2_Effects of acromegaly treatment on left ventricular systolic function assessed by speckle tracking echocardiography in relation to sex differences: results from a prospecti... by Agata Popielarz-Grygalewicz (15424142)

“…</p>Results<p>Treatment with SRL resulted in reduction in median (IQR) GH&IGF-1 levels after 3 months, from 9.1(3.2-21.9) to 1.8(0.9-5.2) ng/mL (p<0.001) and from 3.2(2.3-4.3) to 1.5(1.1-2.5) xULN (p<0.001), respectively. …”