Inhibition of autocrine TGFβ2 signaling prevents reduction of capillary morphogenesis by miR-30b. by Grant A. Howe (2558611)

“…<p>(A) HUVECs were serum starved overnight in MCDB 131 with 0.5% FBS and stimulated with VEGF (50 ng/ml) in the presence or absence of Avastin (1 μg/ml) for 24 hours. …”

HLA-G expression in MSC. by Massimo Giuliani (208267)

“…(<b>a</b>) The RT-PCR products from BM-MSC and FL-MSC, as a result of using specific primers for HLA-G1 and HLA-G5, were analyzed on an agarose gel. A FON-57 cell line and β-actin were used as positive controls for HLA-G1 and HLA-G5, and as a loading control, respectively. …”

Mitochondrial Ca²⁺ uptake in permeabilized H295R cells transfected with <i>OPA1</i> or <i>Mfn1</i> siRNA. by László Fülöp (203208)

“…<p>The cells were transfected with control RNA or siRNA on the day following plating (day 2) and with inverse Pericam targetted into the mitochondria (mt-inv-Pericam) on day 2 or 3. On day 5 the cells were permeabilized, superfused with a cytosol-like medium, Ψ_m was dissipated with 10 µM rotenon, 8 µg/ml oligomycin, 10 µM FCCP and 50 ng/ml valinomycin for 2 minutes. …”

Phenotypes of the shACADS and shNTC knockdown Huh7 cells. by Kerstin Matejka (6729155)

“…Western blotting analysed 10 μg of cell lysate per sample depicted exemplary for one (C) of four experiments (B). ** = p < 0.01 (one sample t-test). mRNA (A) and Protein (B) were harvested after 3 or 5 days of treatment with 0, 5, and 10 ng/mL doxycycline, respectively. *** = p < 0.001 (one-sample t-test). …”

Luminex results of cytokines regulated by IL-1Ra. by Connie S. Chamberlain (440242)

“…By day 11, inhibition of the IL-1R resulted in a down-regulation of IL-1α (a) and tended to decrease IL-1β (b; p = .056). IL-1Ra significantly decreased the pro-inflammatory cytokines, IL-12 (c), IL-2 (d), and IFN-γ (e) on day 5. …”

QUALITY OF LIFE AFTER LAPAROSCOPIC SLEEVE GASTRECTOMY USİNG BAROS SYSTEM by Cüneyt KIRKIL (5670545)

Effect of TIM-3 blockade on uterine MDSC population. by Sudipta Tripathi (67467)

“…<p>Treatment with RMT3-23 did not change the size of the MDSC population in the uteri of pregnant CBA mice (C) although a significant decrease in the proinflammatory S100A8 (p = 0.0003) (A) and A9 (p<0.0001) (B) proteins in the placentae was observed. …”

Analysis of PER phosphorylation sites in S2 cells. by David S. Garbe (463622)

“…Of the mutant dPERs tested, PER-S613A, -T826A, -S1080A and –S1187A display a significant decrease in repressor activity, with PER-T826A exhibiting the most robust change and significance.…”

Inhibition of CXCL8 reduces proliferation, viability and invasion (Experiments vs simulation results). by Junho Lee (220469)

“…<p>(A) Time course of cell proliferation shows a drastic decrease in the LS174T cell population with CXCL8 knockdown (shCXCL8; blue) compared to control (LS174T) in <i>in vivo</i> experiments [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1008257#pcbi.1008257.ref100" target="_blank">100</a>]. …”

Changes in orientation tuning width with different contrasts in mixed contrasts blocks. by Lionel G. Nowak (373941)

“…Relative to 100%, all distributions are shifted to the left, indicating decreased HWHH with decreased contrast. …”

Infection detection strategies differ in ability to reduce transmission. by Jaline Gerardin (845738)

“…<p>(A) Success of infection detection strategies at depleting the infectious reservoir. …”

Shortened Leukocyte Telomere Length Is Associated With Glycemic Progression in Type 2 Diabetes: A Prospective and Mendelian Randomization Analysis by Feifei Cheng (8985345)

Knockouts of <i>gpr-3</i> lack cAMP signaling response to melatonin. by Cathryn S. D. Maienza (20629929)

“…<p>Difference in relative cAMP concentration between <i>gpr-3</i>^<i>+</i> (N380-1, n = 6), <i>gpr-3</i>^{<i>-</i> (}N380-2, n = 6), and <i>gna-1</i>^<i>-</i>(n = 6) strains after 10-minute treatment with 5ng/mL melatonin in sterile water. Untreated group was treated only with water. …”

Celastrol has an inhibitory effect on TNFα-induced NF-κB signaling in MDA-MB-231 cells. by Laura Mezzanotte (223692)

“…<p>(3.A) Graph representing the decrease in fold NF-κB induction in MDA-MB-231 cells using celastrol by the multicolor assay (* p value <0.05; ** p value <0.01). (3.B) Composite image of the unmixed spectrum of luciferases: green signals represent CBG99 emission, red signals represent PpyRE9 emission spectrum and blue signals represent ExtGluc expression in control cells (first column) and cells treated with TNFα in combination with increasing concentrations of celastrol (0.1–3 µM). (3.C) Left: graph reporting the relative band intensity of p65 protein from the nuclear extract of control sample and samples treated with TNFα (10 ng/ml) or TNFα + celastrol (0.1 µM; 0.5 µM and 1 µM). …”

Melatonin Preserves Blood-Brain Barrier Integrity and Permeability via Matrix Metalloproteinase-9 Inhibition - Fig 9 by Himakarnika Alluri (2735689)

“…Animals were divided into sham (n = 6), vehicle + sham (n = 6), vehicle + TBI (n = 5) and melatonin + TBI (n = 6). Data are expressed as ng/brain cortex ± SEM. ‘*’ indicates statistical significance. …”

TGF-β₁ dependent effects on AP activity by SB431542 are depending on time. by Sabrina Ehnert (359631)

“…<p>Primary human osteoblasts stimulated with 5 ng/ml hra TGF-β₁ in the presence or absence of 20 nM SB431542. …”

Phenotypes of ovary cultures treated with FST288. by Zhengpin Wang (147664)

“…<p>Ovaries at 17.5 dpc were cultured for 7 days without treatment (as a control) or with 500 ng/mL FST288. …”

Biophysical characterization of protective effects of Se. by Ramanpreet Kaur (1662517)

“…<p>(a) Demonstrates significant decrease in membrane fluidity in Se-D group in comparison with Se-Ade and Se-Sup groups (b) shows changes in the Histidine transport levels across the epithelial membrane (c, d) shows significant decrease in impedance at 1KHz and 100Hz in Se-Def group in comparison with Se-Ade and Se-Sup groups in series and parallel respectively. …”

Human tau overexpression impaired adult hippocampal progenitor cell (HPC) proliferation. by Kaushik Ghosal (244754)

“…However, overexpression of human tau (hTau) alone also decreased HPC proliferation in wild-type animals and had a tendency to decrease HPC proliferation in AICD animals. n = 4–7 for each group. …”

Serum brain-derived neurotrophic factor reflects the lower inflammation, lipid level, and major adverse cardiac and cerebrovascular events risk in maintenance hemodialysis patients by Hui Du (639270)

“…Our study shows that BDNF was notably (more than two folds) decreased in MHD patients compared with that in HCs (median (interquartile range) value: 5.5 (3.1–9.4) vs. 13.2 (9.4–19.1) ng/mL). …”