SETD2 mediates the binding of MALAT1 and pSmads. by Jinqiang Zhang (709289)

“…<b>B,</b> RNA immunoprecipitation of MALAT1 by using anti-Smad2/3 or anti-SETD2 antibodies in Hep3B cells (with siSETD2 or siControl transfection) treated with 5ng/mL TGF-β1 or vehicle for 2 hours. The precipitated MALAT1 was assessed by qRT-PCR. …”

Confirmation of Functionality of Constructs and Model System Used by Maarten F Bijlsma (380371)

“…</p> <p>(C) Shh concentration in medium is below the detection limit (5 ng/ml) of Western blotting. Medium was spiked with decreasing concentrations of recombinant Shh, and blotted along with a 4× concentrated medium sample obtained from C3H/10T1/2 fibroblasts (incubated for 16 h at a volume-to-surface ratio identical to the mix-and-match and medium transfer experiments).…”

(A) Schematic presentation of the human AID protein sequence along with the positions of the lysine residues mutated in the various constructs by Said Aoufouchi (49316)

“…(D) Stable BL2 clones expressing the indicated AID-EGFP lysine mutants, WT-AID-EGFP, or mutNLS-AID-EGFP were incubated with cycloheximide (CHX, 50 μg/ml) and LMB (10 ng/ml). The decay of the protein was followed by the decrease in fluorescence intensity. …”

Concentration dependent reduction of IL-6 and CXCL8 by BET inhibitors. by Younis M. Khan (554747)

“…These parameters were used to assess cell viability following treatment with JQ1 (0.5 µM) and PFI-1 (1 µM) for 16 hours. DMSO/Control (<1%) alone, PFI-1, JQ1(-) or JQ1 resulted in only 5% decrease of overall cell viability. …”

Table_1_Non-invasive Biomarkers of Liver Inflammation and Cell Death in Response to Alcohol Detoxification.DOCX by Manuela G. Neuman (11086812)

Abrogation of TGF-β1-induced growth inhibition by PGE2. by Erina Takai (465811)

“…Treatment with TGF-β1 decreased the ratio of S phase cells. (B-D) Twenty-four hours after incubation with or without TGF-β1 (5 ng/mL), cells were supplemented with PGE2 (10, 25 µM) and further incubated for 2 days. …”

Kallikrein 6 as a Serum Prognostic Marker in Patients with Aneurysmal Subarachnoid Hemorrhage by Eduardo Martínez-Morillo (132848)

“…There were significant differences between patients with good recovery or moderate disability (n = 8) and patients with severe disability or death (n = 5) (mean values of 1.03 ng/mL versus 0.47 ng/mL, respectively) (p<0.01). …”

H4R-mediated inhibition of clonogenic cell proliferation and/or differentiation. by Anne-France Petit-Bertron (261234)

“…Cells were seeded into MethoCult supplemented with 1 ng/ml of mrIL-3 to generate granulocyte/macrophage colonies or with SCF (100 ng/ml)+IL-7 (10 ng/ml+Flt-3-L (20 ng/ml) to reveal common lymphoid progenitors (CLP). …”

DZNep-mediated sensitization to TRAIL correlates with enhanced activation of caspase signaling and involvement of the mitochondrial apoptosis pathway. by Frank K. Braun (698401)

“…(<b>C</b>) Activation of the mitochondrial apoptosis pathway was determined by TMRM staining for mitochondrial membrane potential (Δψ_m) in Z-138 cells treated with DZNep (0.2, 1, 3, or 5 μM) alone for 40 h. (<b>D, E</b>) Analysis of Δψ_m of cells (Z-138, BJAB, and SU-DHL-9) treated with DZNep (1 μM, 24 h) followed by TRAIL (10 ng/ml for 1–5 h or 20 ng/ml for 16 h). …”

Table1_Retrospective Recall of Psychological Distress Experienced During the First COVID-19 Lockdown in Italy: Results From the ALT RISCOVID-19 Survey.doc by Francesca Bracone (12014342)

“…</p><p>Methods: Cross-sectional analysis on 1,880 adults (mean age 48.9 ± 14.5 years) from the web-based ALT RISCOVID-19 survey. …”

Nutritional profile of patients in a multidisciplinary treatment program for severe obesity and preoperative bariatric surgery by Fernanda Cristina Carvalho Mattos MAGNO (6380201)

“…RESULTS: From the total, 73% of the patients were women and 27% male, mean age was 48.4+12.9 and 49.8+8.1, respectively. …”

Predicted change in current financial strain between M2 (2004–05) and M3 (2013–14) by changes in assets. by Dana A. Glei (1939324)

“…The difference (M3—M2) in log assets is equivalent to the log of the ratio of assets at M3 relative to M2. Thus, a 0.7 decrease in log assets represents a loss of 50% (24% of the observed sample exhibited a loss of that magnitude or greater), whereas a 0.7 increase corresponds with an approximate doubling of assets (28% of the sample reported a gain of that magnitude or greater). …”

Relative expression of target genes normalized with different numbers of reference genes. by Daijun Ling (188975)

“…(B) The age-dependent increased <i>Hsp70</i> expression also shows minor decrease when normalized sequentially by 1, 3 or 13 reference genes. …”

Exemplar press-related channel. by David T. Bundy (7586414)

“…Mean firing rates show a complex task response with firing rates significantly (p<0.05) decreased around the lever press and 650 ms prior to the reach to the pellet and then significantly increased immediately prior to the reach to the pellet. …”

FOXO3a down-regulates <i>PRNP</i> expression by binding to its promoter. by Ting Liu (45625)

“…All experiments were performed three to five times, and the data were analyzed as the mean ± S.E.M. (P<0.05).</p>…”

The defects of OB in Lgl1 mutants. by Zhenzu Li (3108309)

“…Scale bars represent 1mm in Ac and 200 μm(0), 100 μm (a,c) and 50 μm (b,d) in C.</p>…”

<i>Apoe</i> expression is reduced in the parietal cortex and CA1 of aged mice. by Ileana Soto (531878)

“…Values in (B, D, and F) are relative mean <u>+</u> SEM to the young values, <i>n</i> = 6 per group. …”

Potential Impacts of Climatic Change on European Breeding Birds by Brian Huntley (38285)

“…Even if potential range changes were realised, the average number of species breeding per 50×50 km grid square would decrease by 6·8–23·2%. …”

The STD diet reduced the proportion of γδ T cells. by Julie Christine Antvorskov (329686)

Figure 2 by Kazuo Konishi (195884)

“…Horizontal lines represent mean methylation levels for each group. ¶, <i>P</i> = .013. …”