Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Characterization of Heterogeneity and Dynamics of Lysis of Single Bacillus subtilis Cells upon Prophage Induction During Spore Germination, Outgrowth, and Vegetative Growth Using R... by Mei-yan Wu (9921201)

“…Major findings of this work are as follows: (i) After addition of xylose to trigger prophage induction, the intensities of Raman spectral bands associated with nucleic acids of single cells in induced cultures gradually fell to zero, in contrast to the much smaller changes in protein band intensities and no changes in nucleic acid bands in uninduced cultures; (ii) the nucleic acid band intensities from an individual induced cell exhibited a rapid decrease, following a long lag period; (iii) after the addition of nutrient-rich medium with xylose, single spores underwent a long period (228 ± 41.4 min) for germination, outgrowth, and vegetative growth, followed by a short period of cell burst in 1.5 ± 0.8 min at a cell length of 8.2 ± 5.5 μm; (iv) the latent time (<i>T</i>_latent) between the addition of xylose and the start of cell burst was heterogeneous in cell populations; however, the period (Δ<i>T</i>_burst) from the latent time to the completion of cell lysis was quite small; (v) in a poor medium with l-alanine alone, addition of xylose caused prophage induction following spore germination but with longer <i>T</i>_latent and Δ<i>T</i>_burst times and without cell elongation; (vi) spontaneous prophage induction and lysis of individual cells from spores in a minimal nutrient medium were observed without xylose addition, and cell length prior to cell lysis was ∼4.1 μm, but spontaneous prophage induction was not observed in a rich medium; (vii) in a rich medium, addition of xylose at a time well after spore germination and outgrowth significantly shortened the average <i>T</i>_latent time. …”

Integrity of AWC cilia structure is critical for proper localization of EGL-4. by Damien M. O'Halloran (185919)

“…Calcium levels in AWC have been shown to decrease after ligand binding <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000761#pgen.1000761-Chalasani1" target="_blank">[13]</a>. …”

Reversal of ROS induced autophagic vacuole formation in F-HABP07 upon polymeric HA supplementation. by Paramita Saha (473954)

“…<p>[<b>A to D</b>] <b><i>Polymeric </i><i>HA </i><i>supplementation </i><i>restores </i><i>cellular </i><i>homeostasis </i><i>in </i><i>F-HABP07 </i><i>cells</i></b>-- As a result of significant reduction in ROS generation as evident from the decreased fluorescence intensity upon H₂DCFDA treatment (<b>A</b> and <b>B</b>); about 25% revival of growth was observed in F-HABP07 cells (<b>C</b>). …”

Proof of Concept, Randomized, Placebo-Controlled Study of the Effect of Simvastatin on the Course of Age-Related Macular Degeneration by Robyn H. Guymer (168403)

“…<i>Intent to treat</i> multivariable logistic regression analysis, adjusted for age, sex, smoking and baseline AMD severity, showed a significant 2-fold decrease in the risk of progression in the simvastatin group: OR 0.43 (0.18–0.99), p = 0.047. …”

Data_Sheet_1_Coordinated Reset Vibrotactile Stimulation Induces Sustained Cumulative Benefits in Parkinson’s Disease.pdf by Kristina J. Pfeifer (10095703)

“…EEG recordings observed from study 1 indicated a significant decrease in off-medication cortical sensorimotor high beta power (21—30 Hz) at rest after 3 months of daily noisy vCR therapy. …”

Image_1_Defective Autophagy in T Cells Impairs the Development of Diet-Induced Hepatic Steatosis and Atherosclerosis.TIF by Jacob Amersfoort (6075341)

“…Taken together, T cell-specific deficiency of Atg7 resulted in a decrease in hepatic steatosis and limited inflammatory potency in the (naïve) T cell compartment in peripheral lymphoid tissues, which was associated with a strong reduction in experimental atherosclerosis.…”

Absence of LAMP5 disrupts olfaction and leads to anxiety-like behavior. by Marie-Catherine Tiveron (272288)

“…Habituation occurs similarly for both WT and KO mice as shown by a significant decrease in investigation time between the first (Oh1) and last (Oh4) trials (*, p<0.05; **, p<0.01; ***, p<0.001). …”

Progressive differences between IPCs and OC surface proportions promoted 2° and 3° formation. by David E. Larson (246516)

“…‘No OC Expansion’, No Death' curve indicates constant OC surface area (no expansion) and no cell death. (<b>C</b>) A graph indicating the decrease in cell number for the different simulations. …”

<i>NDUFS7</i> depletion in motor neurons affects synapse stability. by Bhagaban Mallik (12459375)

“…The motor neuron-depleted <i>NDUFS7[RNAi]</i> larvae showed a decrease in the number of Futsch-positive loops as compared to the Gal4 control. …”

Defect Tolerance to Intolerance in the Vacancy-Ordered Double Perovskite Semiconductors Cs₂SnI₆ and Cs₂TeI₆ by Annalise E. Maughan (1694959)

“…However, even though tellurium substitution increases electronic dispersion via closer I–I contact distances, the substitution experimentally yields insulating behavior from a significant decrease in carrier concentration and mobility. …”

Pi across filarial nematode species and model organisms. by John Mattick (544773)

“…<i>melanogaster</i> also has a decrease in Pi; it is a small chromosome sometimes referred to as the dot chromosome that is largely heterochromatic and may formerly have been a sex chromosome [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0009838#pntd.0009838.ref074" target="_blank">74</a>].…”

Defect Tolerance to Intolerance in the Vacancy-Ordered Double Perovskite Semiconductors Cs₂SnI₆ and Cs₂TeI₆ by Annalise E. Maughan (1694959)

“…However, even though tellurium substitution increases electronic dispersion via closer I–I contact distances, the substitution experimentally yields insulating behavior from a significant decrease in carrier concentration and mobility. …”

Prediction performance of each model. by Ahmed S. Alkhalifah (20721713)

“…Further studies to define a safe upper limit for IBW-adjusted MP may help clinicians optimize mechanical ventilation settings and decrease the risk of VILI and mortality.…”

Data_Sheet_1_Air pollution exposure and pregnancy outcomes among women with polycystic ovary syndrome.docx by Qianqian Zhu (48100)

“…Each IQR increase in SO₂ among patients without PCOS during periods 1 (start of gonadotropin medication to oocyte retrieval), 2, 5, and 6 (start of gonadotropin medication to serum HCG test) was related to a decrease in clinical pregnancy rate. For the live birth rate, no significant relationship was found between air pollutants, including PM_2.5, PM₁₀, SO₂, NO₂, CO, and O₃, and the live birth rate for women with PCOS. …”

Data_Sheet_1_Acute post-orchiectomy pain does not reduce alpha rams’ interest in feed resources.pdf by Kauany Zorzenon Uzae (18178810)

“…Dominant (alpha) males have priority for accessing limited feed resources, however, the effects of pain on feed interest in members of a group with defined social hierarchy are unknown. …”

POm shows pronounced response disparity to active and passive whisker stimuli. by Anton Sumser (21021061)

“…<p><b>(A)</b> VPM (left) and POm (right) responses to air puffs (“Puff”, teal). …”

Fluorous-Soluble Metal Chelate for Sensitive Fluorine-19 Magnetic Resonance Imaging Nanoemulsion Probes by Amin Haghighat Jahromi (1870612)

“…Shortening <i>T</i>₁ can increase the ¹⁹F image sensitivity per time and decrease the minimum number of detectable cells. We used the condensation between the tripodal ligand tris-1,1,1-(aminomethyl)­ethane and salicylaldehyde to form the <u>sal</u>icylidene-<u>t</u>ris­(<u>a</u>mino<u>m</u>ethyl)<u>e</u>thane chelating agent (SALTAME). …”

Image_1_Interleukin 23 Produced by Hepatic Monocyte-Derived Macrophages Is Essential for the Development of Murine Primary Biliary Cholangitis.jpeg by Debby Reuveni (5633135)

“…</p>Results<p>Flow cytometry analysis of non-parenchymal liver cells in autoimmune cholangitis revealed massive infiltration of the liver by MNPs and neutrophils and a decrease in Kupffer cells numbers. In addition, a 4-fold increase in the incidence of hepatic IL-17A producing CD4⁺ T cells was found to be associated with an increase in hepatic IL23-p19 and IL17A expression levels. …”