Involvement of clathrin-mediated endocytosis in NTBI uptake by PBMCs and HepG2 cells. by Joao Arezes (488491)

“…<p>(A–B) ⁵⁵Fe uptake by PBMCs and HepG2 cells in the presence of 0.45M of sucrose (A) or 80 µM of Dynasore (B) to inhibit clathrin-mediated endocytosis, after incubation with 5 µM of ⁵⁵Fe-citrate (5∶100) at 37°C (C–D) ¹²⁵I-Transferrin internalization in the presence of 0.45M of sucrose (C) or 80 µM of Dynasore (D). …”

Data_Sheet_1_Brain Activity Associated With Regulating Food Cravings Predicts Changes in Self-Reported Food Craving and Consumption Over Time.DOCX by Nicole R. Giuliani (9632423)

“…However, most research on this topic is limited to one follow-up time point, and single outcome measures. The present study seeks to add to that literature by employing a more refined assessment of food craving and consumption outcomes along with a more detailed neurobiological model of behavior change over several time points. …”

Table_1_Transcriptome analysis provides insights into the response of Lotus corniculatus roots to low-phosphorus stress.xlsx by Xin Zhao (71840)

“…Meanwhile, the main pathways, such as carbohydrate metabolism, acid phosphatases, phosphate transporters and biosynthesis of secondary metabolites, as well as related genes were also screened by performing a KEGG enrichment analysis.</p>Discussion<p>The findings provide an essential point of reference for studying the physiological and molecular mechanism of the response to low-P stress in L. corniculatus.…”

Limb survival dependence on Tie2 in the autoamputation model. by Michael Lekas (134183)

“…(f) Quantification of adductor muscle perfusion capacity on day 14 expressed as a vessel score that is a composite of vessel branching point number, length and end points number. …”

MSR algorithm analysis. by Federico Comoglio (224744)

“…(B) The mean move length significantly decreases as a function of the protein length. …”

Molecular pathways underlying degeneration in synapse-enriched fractions are conserved across diverse neurodegenerative conditions. by Thomas M. Wishart (138237)

“…Representative bands from quantitative fluorescent western blot experiments on synapse-enriched fractions from wild-type (WT) and R6/2 mice. C. Bar chart (mean±SEM) showing quantitative fluorescent western blot data for protein expression levels in synapse-enriched fractions from the cerebellum generated from a mouse model of spinocerebellar ataxia type 5 (βIII-spectrin KO mice) compared to wild-type controls (N≥3 mice per genotype). …”

Increased aerobic glycolysis in MADD fibroblasts and <i>xav</i> mutants. by Yuanquan Song (238595)

“…<p><b>A.</b> ECAR was measured in intact control and MADD Patient 1 fibroblasts under basal conditions, following the addition of the mitochondrial inhibitor oligomycin (0.5 µg/ml), in the presence of the uncoupler CICCP (3 µM) and following the addition of the complex I inhibitor rotenone (100 nM). …”

Vulnerability to high fat diet-induced obesity. by Alessandro Bartolomucci (165364)

“…At baseline, when mice were fed standard diet, all experimental groups showed a decrease in body weight, while controls (Con) showed a slight increase (F(3,23) = 3.2, p<0.05). …”

Polymerization kinetics of <i>Plasmodium</i> actins. by Andrea J. Lopez (4990781)

“…Raw data related to panels a-d can be found in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1011174#ppat.1011174.s028" target="_blank">S5 Data</a>, panels e and f in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1011174#ppat.1011174.s029" target="_blank">S6 Data</a>, and panels g and h in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1011174#ppat.1011174.s030" target="_blank">S7 Data</a>.…”

Selective Enrichment of Cysteine-Containing Phosphopeptides for Subphosphoproteome Analysis by Mingming Dong (1471396)

“…Interestingly, amino acid residues surrounding the identified phosphosites were enriched with buried residues (L, V, A, C) while depleted with exposed residues (D, E, R, K). …”

Measurement of mitochondrial function in nasal fibroblasts with and without TGF-β1 or with and without PX-478 To investigate whether mitochondrial function are elevated by TGF-β1,... by Min-Sik Jo (14636809)

“…<p>Oxygen consumption rate (OCR) was significantly increased at 5 ng/ml TGF-β1 (S1A Fig). To determined whether PX-478 decrease mitochondrial function, PX-478 (20 nM) was pretreated for 1 hour, followed by TGF-β1 48 hours. …”

DataSheet_2_Molecular characterization of cross-kingdom RNA interference in Botrytis cinerea by tomato small RNAs.xlsx by Si Qin (3627863)

“…In order to study the functionality of this plant sRNA in reducing the Bcspl1 transcript level, we generated a fungal mutant that contained a 5-nucleotide substitution that would abolish the interaction between the transcript and the sRNA without changing the encoded protein sequence. …”

Modelling of the effects of En1 and En2 on hippocampal cells. by Asma Soltani (4356841)

“…In normal conditions (black lines), low levels of endogenous secreted En1 (from GABA cell for instance) could participate to dendritogenesis (1), spinogenesis (2) (insert), and mTOR signaling (3) in neighbor glutamatergic neurons. In a pathological context, such as autism (red lines), a rise of En2 expression and secretion would generate additional effects, i.e. the increase in dendritic complexity of GABA cells (4), a decrease of clustered PSD95 (5), and the mispairing of glutamatergic synapses (6) (insert). …”

cGMP-Dependent Protein Kinase Type I Is Implicated in the Regulation of the Timing and Quality of Sleep and Wakefulness by Sonja Langmesser (57421)

“…Moreover, PRKG1 deficiency results in an aberrant pattern, and consequently a reduced quality, of sleep and wakefulness, possibly due to a decreased wake-promoting output of the circadian system impinging upon sleep.…”

Altered Cerebrospinal Fluid Proteins in Smith–Lemli–Opitz Syndrome by Wenping Li (440398)

Olfactory Bulb Glomerular NMDA Receptors Mediate Olfactory Nerve Potentiation and Odor Preference Learning in the Neonate Rat by Rebecca Lethbridge (173780)

“…<em>Ex vivo</em> experiments using bulbs from trained rat pups reveal an increase in the AMPA/NMDA EPSC ratio post-training, consistent with an increase in AMPA receptor insertion and/or the decrease in NMDAR subunits. These results support a model of a cAMP/NMDA interaction in generating rat pup odor preference learning.…”

Use of 2-Aminopurine Fluorescence To Examine Conformational Changes during Nucleotide Incorporation by DNA Polymerase I (Klenow Fragment)^† by Vandana Purohit (2947848)

“…Finally, when the primer had a 3‘-OH, a fluorescence decrease with a rate equal to the rate of nucleotide incorporation was observed with both 0 and +1 position reporters. …”

An immunohistochemically stained photomicrograph of the stomach mucosa using an anti-TLR4 antibody. by Rehab F. Taher (20478600)

“…<p>(a) few TLR4-positively stained cells in the normal group; (b) the control ethanol group showed elevated TLR4 expression and a higher proportion of positive cells having deep brown coloring, (c) Omeprazole group showed reduction in the percentage of TLR4 positive cells with strong brown coloring, (d) PM-EtOH (25 mg/kg) group showed lowered percentage of TLR4 positive cells with weak cytoplasmic staining, (e) PM-EtOH (5o mg/kg) group showed decreased percentage of TLR4 positive cells, (f) PM-EtOH (100 mg/kg) group giving weak TLR4 expression, with a significant decrease in TLR4-positively stained cells(TLR4 immunohistochemical staining; Scale bar=100µm).…”

An Inducible Cell-Cell Fusion System with Integrated Ability to Measure the Efficiency and Specificity of HIV-1 Entry Inhibitors by Alon Herschhorn (198447)

“…Target cells express the HIV-1 receptors, CD4 and CCR5, and carry the firefly luciferase (F-Luc) reporter gene under the control of a tTA-responsive promoter. …”

Circular Dichroism analysis, PPIase activity and nickel regulation of <i>H</i>. <i>pylori</i> WT and mutant SlyD proteins. by Milica Denic (9987685)

“…<p><b>A.</b> Circular Dichroism spectra of SlyD wild type and mutant proteins were analyzed in 5 mM phosphate buffer (pH = 7.6). …”