Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

Tandem Imaging of Breath Ethanol and Acetaldehyde Based on Multiwavelength Enzymatic Biofluorometry by Kenta Iitani (4175995)

“…The oxidation of EtOH by ADH_OX in the presence of NAD⁺ produced NADH, which was subsequently oxidized by diaphorase (DP) with resazurin, leading to the resorufin formation, characterized by red fluorescence (excitation at 560 nm and fluorescence at 590 nm). Reduction of AcH by ADH_RD consumed NADH, leading to a decrease in blue fluorescence (ex. 340 nm, fl. 490 nm). …”

WT data of set A398E/V and set M1425V/I pooled for better comparison. by Yousra El Ghaleb (22075892)

Subjects:

Typical tracing of the effect of i.a. endothelin 0.5 10⁻⁵ M on arterial pressure in rats: the initial transient decrease of arterial pressure is followed by a sustained increase (Fig 2A). by Matthieu Roustit (315980)

FABP5 expression levels in brain microvessels from 2-, 8-, 12-, and 24-month-old mice. by Takuro Iwao (14636741)

Subjects:

Absence of adaptive immunity decreases tumor cell damage in MDR1A deficiency. by Eva Maria Hennenberg (4234018)

LC/MS/MS results for vitamin D analysis. by Jessica S. Brown (15021996)

Pharmacologic blockade of 5LO decreases Aβ peptides levels and deposition in the 3×Tg mice via the γ-secretase pathway. by Jin Chu (358006)

The spike-in of synthetic hsa-lest-7a-5p miRNA decreases the Ct Values of extracted small RNA samples. by Marc Van Der Hofstadt (1423027)

Fig 5 - by Liang Cao (135383)

Decreased co-localization of Py with the late endosome and lysosome in GD1a-supplemented cells. by Mengding Qian (263231)

Unusual [3+1] Cycloaddition of a Stable Silylene with a 2,3-Diazabuta-1,3-diene versus [4+1] Cycloaddition toward a Buta-1,3-diene by Yun Xiong (1403545)

“…The latter rearranges further to decrease ring strain, affording the corresponding 1-sila-4,5-diazacyclohex-3-ene <b>4</b>. …”

RSV decreased the Cav-1 and TGF-β expression in liver of rats fed high-fat diet. by Hui Yang (91136)