Model for simultaneous analysis of structural damage and repair as well as copy number change in stressed cells using real-time PCR by Jinsong Chen (25538)

“…</p> () Real-time PCR allows sensitive detection of relaxed (open circular/linear) or total mtDNA when all supercoiled DNA is converted into the relaxed forms using a heat-denaturation step. The ratio between relaxed and total mtDNA indicates the baseline levels of structural damage, while the quantitative differences between total and relaxed mtDNA represents the fraction of supercoiled DNA. () Induced structural damage and its subsequent repair can be detected by the increase or decrease of the relaxed fraction of mtDNA when total mtDNA content remains the same (I). …”

Treatment of simulated crush injury to nodes on the right with a potassium channel blocker that reduces peak potassium conductance in all nodes by 80%. by Charles F. Babbs (430220)

“…Average axon diameter 1.0 micrometer; number of nodes of Ranvier 21; normal node width 0.65 micrometer; injured node width 1.95 micrometer; width of juxtaparanodal region on both sides of a node 5.0 micrometers. The resistivity of intracellular fluid 200 ohm-cm; normal paranodal resistance 3.2×10¹⁰; injured paranodal resistance 3.2×10⁸ ohms; specific membrane capacitance of axonal membrane 1 mircofarad per square centimeter; resting axonal membrane potential –85 mV; threshold potential –50 mV; sodium equilibrium potential +67 mV; potassium equilibrium potential –95 mV. …”

Voltage gating is intrinsic to Cx46 hemichannels by Vytas K. Verselis (53583)

“…When the extracellular solution was essentially devoid of external divalent cations, V gating at +90 mV was unaffected and loop gating at −70 mV was abolished, but application of a very large hyperpolarizing step to −160 mV caused a robust and rapid decrease in current. …”

The effect of dendritic distribution of CaL on PIC patterns and firing types. by Yi Cheng (317041)

“…<p><b>A.</b> PICs were induced by bi-ramp voltage when the dendritic length was extended to 100% (black, control), 300% (blue), and 500% (dark blue) with distal distribution of CaL. …”

Antioxidant changes (RS) induced previous thermal denaturation. by Rafael Medina-Navarro (253862)

“…The antioxidant capacity (AC) of the albumin previously placed at 65°C was 94 nM TEU and corresponds to 100% of ACA%. RS correspond to ACA%-100 as specified in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0008971#s3" target="_blank">Material and Methods</a> section. …”

A mathematical model of <i>Clostridium difficile</i> transmission in medical wards and a cost-effectiveness analysis comparing different strategies for laboratory diagnosis and pat... by Vered Schechner (3740008)

“…For each strategy, we calculated the annual cost (of CDI diagnosis and isolation) for a decrease of 1 in the average daily number of CDI patients; the strategy of the two-step test and contact isolation in multiple-bed rooms was the reference strategy.…”

The relationship between the parameters <i>ζ</i>/<i>η</i> of the proposed model and the indicators <i>G</i>, 〈<i>h</i>〉, <i>R</i>, and <i>Y</i> for the novelty waves. by Mikihiro Suda (17616078)

“…<p>The gray lines in the plot represent the range of values for <i>η</i> and <i>ζ</i> ranging from 0.1 to 1.0, with a 0.2 step size. The paired values of <i>ρ</i> and <i>ν</i> range from 1 to 30 with a step size of 5, which results in a total of 49 gray lines. …”

Effect of TIM-3 blockade on uterine MDSC population. by Sudipta Tripathi (67467)

“…<p>Treatment with RMT3-23 did not change the size of the MDSC population in the uteri of pregnant CBA mice (C) although a significant decrease in the proinflammatory S100A8 (p = 0.0003) (A) and A9 (p<0.0001) (B) proteins in the placentae was observed. …”

Analysis of PER phosphorylation sites in S2 cells. by David S. Garbe (463622)

“…Of the mutant dPERs tested, PER-S613A, -T826A, -S1080A and –S1187A display a significant decrease in repressor activity, with PER-T826A exhibiting the most robust change and significance.…”

The status of forward osmosis technology implementation by Awad, Abdelrahman M.

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The First-Year Follow-Up of a Cleft Lip and Palate Patient Treated With Nasoalveolar Molding (NAM) by Nathália Viegas de Oliveira (10439815)

Endoscopic endonasal double flap technique for reconstruction of large anterior skull base defects: technical note by Ricardo Landini Lutaif Dolci (7341128)

Inhibition of CXCL8 reduces proliferation, viability and invasion (Experiments vs simulation results). by Junho Lee (220469)

“…<p>(A) Time course of cell proliferation shows a drastic decrease in the LS174T cell population with CXCL8 knockdown (shCXCL8; blue) compared to control (LS174T) in <i>in vivo</i> experiments [<a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1008257#pcbi.1008257.ref100" target="_blank">100</a>]. …”

Supplementary Material for: Role of 1α,25-Dihydroxyvitamin D₃ in Adipogenesis of SGBS Cells: New Insights into Human Preadipocyte Proliferation by Felicidade I. (3683905)

“…In preadipocytes, 1,25(OH)₂D₃ induced G1 growth arrest at both tested concentrations without altering <i>CDKN1A</i> gene expression. Treatment with 100 nM 1,25(OH)₂D₃ also decreased MTT absorbance and the lipid concentration. …”

Changes in orientation tuning width with different contrasts in mixed contrasts blocks. by Lionel G. Nowak (373941)

“…Relative to 100%, all distributions are shifted to the left, indicating decreased HWHH with decreased contrast. …”

Infection detection strategies differ in ability to reduce transmission. by Jaline Gerardin (845738)

“…<p>(A) Success of infection detection strategies at depleting the infectious reservoir. …”

Supplementary movie 1 from Role of intracellular poroelasticity on freezing-induced deformation of cells in engineered tissues by Soham Ghosh (1319076)

“…Part 2, Time lapse video of a cell in suspension undergoing hydraulic locking. Time step: 0.5 seconds. Note the decreasing cell volume at first, followed by hydraulic locking characterized by constant cell volume. …”

Tracking performance. by Dylan B. Snyder (17691734)

“…<p>Tracking period SD of hand speed adaptation over time with possible step increase in adaptation due to application of tendon vibration. …”

Dynamic and Static Postural Control in Volleyball Players with Transfemoral Amputation by Helmorany Nunes de Araújo (6303473)

“…Methods: Sixteen subjects participated in the study, eight of whom were amputees with unilateral transfemoral amputation (amputee group: age: 33.5 ± 5.6 years, weight: 77.7 ± 5.3 kg, height: 179.4 ± 5.3 cm) and eight physically active non-amputees (control group age: 27.2 ± 8.4 years, weight: 82.7 ± 6.6 kg, height: 178.7 ± 6.1 cm). …”

Establishment of ex vivo cetuximab sensitivity assays. by Takahiro Tashiro (827031)

“…<p>A, Growth of C132 CTOSs cultured with the indicated doses of cetuximab for 7 days. …”