Supplementary Material for: Effect of Progesterone Withdrawal on Hypothalamic Mechanisms Related to Prolactin Release in Late Pregnant Rats by Bonafede M.M. (4127374)

“…<i>Methods:</i> To investigate the hypothalamic mechanism mediating this response, we determined expression of tyrosine hydroxylase (TH), PRL receptors (long form, PRLR_<i>long</i>), estrogen-α (ERα) and ERβ, P₄ (PR) A and B receptors, and STAT5a, STAT5b, suppressors of cytokine signaling 1 (SOCS1), SOCS3 and CIS at mRNA (by semiquantitative and real-time RT-PCR) and protein (by Western blot only for TH, ERα and PRs) levels, and dopamine and DOPAC (by high-performance liquid chromatography) contents in the mediobasal hypothalamus (MBH) 24 h after luteolysis induced by a PGF2α analogue (cloprostenol, 25 µg/rat s.c. at 8 and 12 h on day 19 of pregnancy). …”

Image2_Novel multitarget analgesic candidate SZV-1287 demonstrates potential disease-modifying effects in the monoiodoacetate-induced osteoarthritis mouse model.TIF by Ádám István Horváth (15443678)

“…Introduction<p>Monoiodoacetate (MIA)-induced osteoarthritis (OA) is the most commonly used rodent model for testing anti-OA drug candidates. Herein, we investigated the effects of our patented multitarget drug candidate SZV-1287 (3-(4,5-diphenyl-1,3-oxazol-2-yl) propanal oxime) that is currently under clinical development for neuropathic pain and characterized the mouse model through complex functional, in vivo imaging, and morphological techniques.…”

The Proteasome as a Drug Target in the Metazoan Pathogen, <i>Schistosoma mansoni</i> by Betsaida Bibo-Verdugo (4273624)

“…After 24 h, bortezomib and carfilzomib decreased worm motility by more than 85% and endogenous proteasome activity by >75%, and after 72 h, they increased caspase activity by >4.5-fold. …”

Long-term use of cover crops and no-till shift soil microbial community life strategies in agricultural soil by Radomir Schmidt (1956940)

“…In long-term experimental plots at the West Side Research and Extension Center in California’s Central Valley, we characterized soil microbial communities in the presence or absence of physical disturbance due to tillage, in the presence or absence of cover crops, and at three depths: 0–5, 5–15 and 15–30 cm. …”

Bleomycin-mediated lung injury in wild-type C57BL/6J mice was attenuated by rapamycin (treatment initiated at 5 days before bleomycin injection). by Yao-Song Gui (305895)

“…<p>A) H&E staining (a-d) and Masson’s trichrome staining (e-h) of mouse lungs were performed after bleomycin injection at day 21. …”

DFP treatment of iMEFs. by Holly J. Garringer (281855)

“…<p>Cell viability of iron loaded iMEFs (3 days with 100 μM iron) increased considerably after 3 days of DFP exposure (A). …”

DataSheet_1_Vitamin A deficiency and vitamin A supplementation affect innate and T cell immune responses to rotavirus A infection in a conventional sow model.docx by Juliet Chepngeno (7532261)

“…Clinical signs of RVA were evaluated post inoculation of sows and post-challenge of piglets. We observed decreased frequencies of NK cells, total and MHCII⁺ plasmacytoid DCs, conventional DCs, CD103⁺ DCs and CD4⁺/CD8⁺ and T regulatory cells (Tregs) and NK cell activity in VAD+RVA sows. …”

Image_1_Dose-Dependent Effects of Aloin on the Intestinal Bacterial Community Structure, Short Chain Fatty Acids Metabolism and Intestinal Epithelial Cell Permeability.TIF by Kuppan Gokulan (466481)

“…As a proof of concept, we incubated 3% rat fecal-slurry (an in vitro model to simulate human colon content) with 0.5, 1, and 2 mg/ml of aloin to test antimicrobial properties. …”

Stable diameter maintenance depends on how growth signal width varies with cell diameter (quantified by the value of <i>β</i>, see Eq. (11)). by Tyler Drake (473458)

“…We used , corresponding to and <i>L</i>₀ = 2.5 w₀. …”

Table_2_Head-Down-Tilt Bed Rest With Elevated CO2: Effects of a Pilot Spaceflight Analog on Neural Function and Performance During a Cognitive-Motor Dual Task.docx by Aditya D. Mahadevan (11328372)

“…<p>Spaceflight has widespread effects on human performance, including on the ability to dual task. Here, we examine how a spaceflight analog comprising 30 days of head-down-tilt bed rest (HDBR) combined with 0.5% ambient CO₂ (HDBR + CO₂) influences performance and functional activity of the brain during single and dual tasking of a cognitive and a motor task. …”

Table_1_Head-Down-Tilt Bed Rest With Elevated CO2: Effects of a Pilot Spaceflight Analog on Neural Function and Performance During a Cognitive-Motor Dual Task.DOCX by Aditya D. Mahadevan (11328372)

“…<p>Spaceflight has widespread effects on human performance, including on the ability to dual task. Here, we examine how a spaceflight analog comprising 30 days of head-down-tilt bed rest (HDBR) combined with 0.5% ambient CO₂ (HDBR + CO₂) influences performance and functional activity of the brain during single and dual tasking of a cognitive and a motor task. …”

Image_1_Head-Down-Tilt Bed Rest With Elevated CO2: Effects of a Pilot Spaceflight Analog on Neural Function and Performance During a Cognitive-Motor Dual Task.JPEG by Aditya D. Mahadevan (11328372)

“…<p>Spaceflight has widespread effects on human performance, including on the ability to dual task. Here, we examine how a spaceflight analog comprising 30 days of head-down-tilt bed rest (HDBR) combined with 0.5% ambient CO₂ (HDBR + CO₂) influences performance and functional activity of the brain during single and dual tasking of a cognitive and a motor task. …”

Image_2_Head-Down-Tilt Bed Rest With Elevated CO2: Effects of a Pilot Spaceflight Analog on Neural Function and Performance During a Cognitive-Motor Dual Task.JPEG by Aditya D. Mahadevan (11328372)

“…<p>Spaceflight has widespread effects on human performance, including on the ability to dual task. Here, we examine how a spaceflight analog comprising 30 days of head-down-tilt bed rest (HDBR) combined with 0.5% ambient CO₂ (HDBR + CO₂) influences performance and functional activity of the brain during single and dual tasking of a cognitive and a motor task. …”

Results of phantom eye model. by Makoto Shirasawa (438600)

“…<p>Representative SD-OCT images of phantom eye model (A–C). A, ART = 1; B, ART = 20; C, ART = 100. ART =  number of frames averaged. …”

Drought tolerance of SDG8i plants. by Sharmin Islam (479989)

“…<div><p>(A) Plants were deprived of water, re-watered at intervals and monitored for recovery as described in the text. …”

Sampling design. by Kiwako S. Araki (680112)

“…Vegetation cover data was used as a measure of environmental heterogeneity (a), and the spatial distribution of genets (groups of clonal ramets with shared genotypes) was determined through simple sequence repeat (SSR) analyses (b). …”

Tuning the Attempt Frequency of Protein Folding Dynamics via Transition-State Rigidification: Application to Trp-Cage by Rachel M. Abaskharon (1351644)

“…We find that this strategy not only decreases the folding time of the Trp-cage peptide by more than an order of magnitude (to ∼100 ns at 25 °C) but also exposes parallel folding pathways, allowing us to provide, to the best of our knowledge, the first quantitative assessment of the curvature of the transition-state free-energy surface of a protein.…”

Prevalence and rate of reversion of RT mutations at baseline (BL), 2 months (2mo) and 4 months (4mo) Structured Treatment Interruption (STI) and associated changes in Replication C... by Agnes C. Paquet (231161)

“…Between 2 months and 4 months of STI, the reversion of mutations was also associated with an increase in RC, although of a lesser magnitude since RC was already close to 100% at 2 months (data not shown).…”

Constructions of tryptase lentivirus carrier and tryptase siRNA lentivirus carrier. by Xiuling Zhi (397887)

“…Same fields of vision under an optical microscope(×100). More than 80% cells were infected with lent-GFP at a dose of MOI 10 after 48 h treatment. …”

Classification accuracy under fixed iterations. by Alexander Tscshantz (16734185)

“…The classification accuracy of standard predictive coding slowly decreases over batches, illustrating a common pathology observed in these simulations. …”