Table_2_Differential Profile of Systemic Extracellular Vesicles From Sporadic and Familial Alzheimer’s Disease Leads to Neuroglial and Endothelial Cell Degeneration.XLSX by Juan Villar-Vesga (4900768)

“…We found increase CLN-5 spots with vesicular morphology in the abluminal portion of vessels from SAD patients. …”

Image_2_Osmotic Imbalance, Cytoplasm Acidification and Oxidative Stress Induction Support the High Toxicity of Chloride in Acidophilic Bacteria.png by Javier Rivera-Araya (7554596)

“…On the other hand, the intracellular pH significantly decreased from 6.7 to pH 5.5 and oxygen consumption increased significantly when the cells were exposed to NaCl stress. …”

Image_1_Skeletal Muscle Shape Change in Relation to Varying Force Requirements Across Locomotor Conditions.jpg by Nicolai Konow (207226)

“…Using high-speed bi-planar fluoromicrometry, we analyze in vivo strains within the rat medial gastrocnemius (MG) across a range of gait and slope conditions. …”

Image_1_Osmotic Imbalance, Cytoplasm Acidification and Oxidative Stress Induction Support the High Toxicity of Chloride in Acidophilic Bacteria.PNG by Javier Rivera-Araya (7554596)

“…On the other hand, the intracellular pH significantly decreased from 6.7 to pH 5.5 and oxygen consumption increased significantly when the cells were exposed to NaCl stress. …”

Image_2_Skeletal Muscle Shape Change in Relation to Varying Force Requirements Across Locomotor Conditions.jpeg by Nicolai Konow (207226)

“…Using high-speed bi-planar fluoromicrometry, we analyze in vivo strains within the rat medial gastrocnemius (MG) across a range of gait and slope conditions. …”

Flexible Solid-State Supercapacitors with High Areal Performance Enabled by Chlorine-Doped Graphene Films with Commercial-Level Mass Loading by Hedong Jiang (7547852)

“…To address this challenge, we propose a facile hydrochloric acid (HCl) solvothermal strategy to fabricate high areal performance chlorine-doped reduced graphene oxide films (Cl-RGOFs). …”

Loss of HIF-1α in the Notochord Results in Cell Death and Complete Disappearance of the Nucleus Pulposus by Christophe Merceron (408738)

“…Morphologic changes of the mutant NP cells were identified as early as E15.5, followed, postnatally, by the progressive disappearance and replacement of the NP with a novel tissue that resembles fibrocartilage. …”

DataSheet1_Identification of hypertrophy-modulating Cullin-RING ubiquitin ligases in primary cardiomyocytes.PDF by Maximillian Fischer (14758063)

“…Of 43 targets screened, siRNA-mediated depletion of Fbxo6, Fbxo45, and Fbxl14 resulted in decreased cell size, whereas depletion of Fbxo9, Fbxo25, Fbxo30, Fbxo32, Fbxo33, Cullin1, Roc1, Ddb1, Fbxw4, and Fbxw5 led to a markedly increased cell size under basal conditions. …”

Table1_Identification of hypertrophy-modulating Cullin-RING ubiquitin ligases in primary cardiomyocytes.DOCX by Maximillian Fischer (14758063)

“…Of 43 targets screened, siRNA-mediated depletion of Fbxo6, Fbxo45, and Fbxl14 resulted in decreased cell size, whereas depletion of Fbxo9, Fbxo25, Fbxo30, Fbxo32, Fbxo33, Cullin1, Roc1, Ddb1, Fbxw4, and Fbxw5 led to a markedly increased cell size under basal conditions. …”

Syncytiotrophoblast extracellular vesicles impair rat uterine vascular function via the lectin-like oxidized LDL receptor-1 by Floor Spaans (307972)

“…Pre-incubation of the vessels with a nitric oxide synthase inhibitor (L-NAME) demonstrated that the STBEV-induced impairment in vasodilation was due to decreased nitric oxide contribution (ΔAUC 12.2±11.7 in STBEV-arteries vs. 86.5±20 in controls, p<0.05), which was abolished by LOX-1 blocking antibody (ΔAUC 98.9±17, p<0.05). …”

HIRA Is Required for Heart Development and Directly Regulates <i>Tnni2</i> and <i>Tnnt3</i> by Daniel Dilg (2940339)

“…We found that HIRA binds GAGA rich DNA loci in the embryonic heart, and in particular a previously described enhancer of <i>Tnni2/Tnnt3</i> (<i>TTe</i>) bound by the transcription factor NKX2.5. …”

Data Sheet 1_Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum.xlsx by Karol Mierzejewski (20393026)

“…The CL slices were incubated in the presence of LPS for 24 h.</p>Results<p>We identified 12 differentially regulated proteins after treatment with LPS (7 of them were upregulated, while 5 were downregulated). …”

Predicting thermal damage accumulation in plants by Andreas Faber (12889046)

“…Additionally, thermal tolerance estimates from previous studies were used to create TDT models to assess the applicability of this framework in plants.</p><p dir="ltr">We show that thermal damage is additive between 44 to 47 °C and -6.5 to -8 °C and that the TDT model can predict damage accumulation at both temperature extremes. …”

Simulation of hypGOI expression study in VK2/E6E7 cells treated with <i>Lactobacillus acidophilus</i> NCFM. by Annette V. Jacobsen (677206)

“…The most stable gene assessed (<i>RPLP0</i>) was used as the standard for the target fold changes, which were set at two-fold decrease (A), two-fold increase (B), five-fold decrease (C), and five-fold increase (D). …”

Simulation of hypGOI expression study in HT-29 cells treated with <i>Lactobacillus acidophilus</i> NCFM. by Annette V. Jacobsen (677206)

“…The most stable gene assessed (<i>PGK1</i>) was used as the standard for the target fold changes, which were set at two-fold decrease (A), two-fold increase (B), five-fold decrease (C), and five-fold increase (D). …”

Changes in cell adhesion and in neuronal morphology induced by sAPPα, sAPPβ and sAPP-Fc added to neurons after 1 DIV. by Stéphanie Chasseigneaux (231744)

“…<p>(<b>A</b>) 5DIV neurons were immunostained for MAP2 (green) and Tau (red). …”

Data_Sheet_1_Soil Application of Almond Residue Biomass Following Black Soldier Fly Larvae Cultivation.docx by Robert Axelrod (6307505)

“…Under aerobic conditions, SPH addition increased the CO₂-accumulation by a factor of 5–6 compared to the non-amended soils in SL and CL, respectively. …”

Protocol: Temporary Pacemaker Verification and Troubleshooting by Jill Ley (4090672)

“…<p>(Figure 1)</p> <p>(Figure 2)</p> <p><strong>Determining the Stimulation Threshold</strong></p> <ul> <li>NOTE: a paced rhythm must be present and patient stable</li> <li>From 100% pacing, gradually decrease output for A or V, while watching monitor until you lose capture</li> <li>Now gradually increase output until 1:1 capture returns – this is the stimulation threshold</li> <li>This is the lowest amount of energy that will reliably capture the heart and pace</li> <li>Set mA 1.5-3 times above this threshold value for safety</li> <li>Repeat for other circuit (A or V)</li> <li>Note: the stimulation threshold should be checked DAILY by the MD/NP when pacing is in use; in the event 
the threshold value is rising, discuss with surgeon to determine if alternative pacing system is needed</li> </ul> <p><strong>Determining the Sensing Threshold</strong></p> <ul> <li>Note: the patient must have an underlying rhythm and tolerate a brief period without pacing to perform</li> <li>Set RATE at least 10 bpm below patient’s intrinsic heart rate (to allow sensing)</li> <li>Set OUTPUT at 0.1 mA (to avoid competitive pacing when device is made asynchronous briefly)</li> <li>Highlight SENSITIVITY (under Menu 1)</li> <li>Decrease SENSITIVITY: Slowly turn dial counter-clockwise until pace indicator flashes regularly (asynchronously)</li> <li>Increase SENSITIVITY: Slowly turn dial clockwise until sense indicator flashes again (when sensing resumes, this is the sensing threshold – the smallest complex that is reliably recognized, thus inhibiting the device)</li> <li>Set SENSITIVITY < half this threshold value for safety</li> <li>Repeat for other circuit (A or V)</li> <li>Restore previous rate and output values</li> </ul> <p><strong>Rapid Atrial (Overdrive) Pacing – PERFORMED BY MD/NP/CNS ONLY</strong></p> <ul> <li>Used to “overdrive pace” and terminate some atrial reentrant tachyarrhythmias, especially atrial flutter</li> <li>ALWAYS VERIFY correct wire connections to avoid stimulating the ventricles at a rapid rate!…”

Project Achilles Copy Number Paper by Robin Meyers (2819002)

“…</div><div><br></div><div><br></div><div><br></div><div>Analysis steps v3.3.8a:</div><div><br></div><div>1. QC and identify reps with reproducibility <0.8 or are outliers on PCA or fail FP</div><div>2. remove sgRNAs(guides) with low counts in DNA samples:</div><div>completely remove shRNAs with median <=1 </div><div>3. remove replicates that fail QC measures </div><div>4. fold change(FC) with DNA pool samples </div><div>5. zero-center median of negative controls</div><div>6. collapse replicates per line </div><div>7. sgRNAs are mapped to genes using(Achilles_v3.3.8a.gct)</div><div><br></div><div>Analysis steps v3.3.8:</div><div><br></div><div>1. …”

Proliferation of NK cells following IL-15 treatment of CAST mice. by Patricia L. Earl (8748759)

“…The established gates were then applied to all IL-15- and PBS-treated samples stained with both anti-CD3 and anti-CD335. <b>(B)</b> Decrease in NK cell numbers with time. NK cells were analyzed as in panel A at 1, 3 and 5 days after cessation of IL-15 inoculations and the numbers for individual mice are shown. …”