Chronic treatment of non-small-cell lung cancer cells with gefitinib leads to an epigenetic loss of epithelial properties associated with reductions in microRNA-155 and -200c by Michiko Narita (124347)

“…However, its application is limited by resistance-accelerated disease progression, which is accompanied by the epithelial-to-mesenchymal transition (EMT). In the present study, we performed multiple expression analyses of microRNAs (miRNAs) and quantified the expression of several related EMT players in gefitinib-resistant NSCLC cells.…”

Development to gametes occurs directly from type I meronts, and there is no evidence to support a 4N type II meront as the committed stage. by Elizabeth D. English (7349108)

“…Prior to 16 hours of infection, nearly 100% of the parasites that pass through a 4N stage become 8N prior to egress. This decrease after 40.5 hours of infection, and this decrease is proportional to the increase in parasites that become 16N (male) at these time points (<i>n</i> = 1,095). …”

HCMV infection of NSCs impairs neuronogenic differentiation in vitro. by Maude Rolland (769547)

“…Scale bar: 50μm. (B) Western blot analysis of whole lysates from differentiating NSC infected by live HCMV at a MOI of 10 (HCMV) or by UV-irradiated HCMV (UV), or uninfected controls, showing decreased levels of βIII tubulin (βIII tub) in the infected cultures. …”

<b>Ectomycorrhizal trees rely on nitrogen resorption less than arbuscular mycorrhizal trees globally</b> by Bai Liu (17306110)

Random Lasing in ZnO Nanopowders Based on Multiphoton Absorption for Ultrafast Upconversion Application by Jiao Tian (583054)

“…This study reports ultraviolet picosecond random lasing (RL) based on multiphoton absorption in zinc oxide (ZnO) nanopowders with a mean size of ∼100 nm under femtosecond optical excitation. …”

Hypoxia activates autophagy in CD8 T cells. by Katelin N. Townsend (501754)

“…(B) Autophagy induction is shown by quantification of decreasing p62 levels without chloroquine (CQ) treatment and (C) LC3-II accumulation with CQ treatment under normoxia (norm) or 1.5% oxygen (hyp). …”

Co-localization of Mmi1 with proteasomes. by Mark Rinnerthaler (477426)

“…We conclude that heat shock results in increase of the proteasomal activity and a presence of Mmi1 displays an inhibitory function in regulation of the proteasomal activity which is most pronounced after heat-shock.…”

Integrative Approach Detected Association between Genetic Variants of microRNA Binding Sites of TLRs Pathway Genes and OSCC Susceptibility in Chinese Han Population by Yun Wang (32846)

“…<div><p>Oral squamous cell carcinoma (OSCC) is a leading malignancy worldwide; the overall 5-year survival rate is approximately 50%. …”

Table_3_Effect of Wii Fit Exercise With Balance and Lower Limb Muscle Strength in Older Adults: A Meta-Analysis.DOCX by Haoyan Liu (10779576)

“…The methodologic quality of the included studies evaluated with the PEDro average score was 6.4 and ranged from 5 to 8 points, indicating moderate quality. Compared with no exercise, Wii Fit exercise was associated with significant improved scores on the Berg Balance Scale, which evaluates functional balance (mean difference, 1.38; 95% CI, 0.61–2.16; I² = 31%; P = 0.0005) and on the Timed Up and Go test, which evaluates static and dynamic balance (standardized mean difference, −0.51; 95% CI,−0.88 to −0.14; I² = 52%; P = 0.007). …”

Data_Sheet_1_Effect of Wii Fit Exercise With Balance and Lower Limb Muscle Strength in Older Adults: A Meta-Analysis.docx by Haoyan Liu (10779576)

“…The methodologic quality of the included studies evaluated with the PEDro average score was 6.4 and ranged from 5 to 8 points, indicating moderate quality. Compared with no exercise, Wii Fit exercise was associated with significant improved scores on the Berg Balance Scale, which evaluates functional balance (mean difference, 1.38; 95% CI, 0.61–2.16; I² = 31%; P = 0.0005) and on the Timed Up and Go test, which evaluates static and dynamic balance (standardized mean difference, −0.51; 95% CI,−0.88 to −0.14; I² = 52%; P = 0.007). …”

Lizards learn a spatial task in a closed-loop automated paradigm. by Tal Eisenberg (18078801)

“…Notice the strong increase for the third area (blue) and strong decrease for the second area. <b>(F)</b> Position trajectories over a single day (animal 1, block 3, day 3), segmented into stationary (gray dots marking mean location during stationary periods) and movement periods (colored lines). …”

Down-regulation of IRP1 expression and IRP1 activities in mouse macrophages exposed to paraquat (PQ). by Anna Milczarek (4036724)

“…<b>(D)</b> IRP1 aconitase activity (means ± S.D. n = 5 biological experiments) determined spectrophotometrically in hepatic cytosolic extracts by measuring the disappearance of <i>cis</i>-aconitate at 240 nm as described previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0176800#pone.0176800.ref012" target="_blank">12</a>]. …”

Mitochondrial Ca²⁺ uptake contributes to cell death induced by Aβ oligomers. by Sara Sanz-Blasco (271498)

“…Cells were loaded with TMRM 10 nM and mitochondrial depolarization was estimated by the decrease in TMRM fluorescence. Pictures show TMRM fluorescence images of cerebellar granule cells treated for 5 min with vehicle (control), 100 nM FCCP or 10 µM FCCP. …”

Data Sheet 1_Lipoxin A4 levels correlate with severity in a Spanish COVID-19 cohort: potential use of endogenous pro-resolving mediators as biomarkers.docx by Sergio Sánchez-García (16862127)

“…We found reduced levels in deceased patients when compared to mild or severe patients, concomitant with a decrease in the LXA₄ biosynthetic pathway and an increase in its degradation pathway. …”

Manchette-acrosome disorders during spermiogenesis and low efficiency of seminiferous tubules in hypercholesterolemic rabbit model by Layla Simón (289208)

“…Tubular efficiency was determined by comparing total number of spermatogenic cells with each cell type within the proliferation/differentiation compartments. We found lower testicular efficiency related to both a decrease in spermatogonial cells and an increase in germ cell apoptosis in hypercholesterolemic rabbits. …”

Image 6_Divergent B-cell and cytotoxic TNK cell activation signatures in HLA-B27-associated ankylosing spondylitis and acute anterior uveitis.jpeg by Eisa Mahyari (9681962)

“…Using cellular indexing of transcriptomes and epitopes (CITE-Seq) in a well-characterized cohort of 25 subjects—including AS (HLA-B27^pos), AS+AAU (HLA-B27^pos), AAU (HLA-B27^pos), HCs (HLA-B27^pos), and HCs (HLA-B27^neg); N = 5/group—we identified transcriptomic differences at the single-cell level, along with differentially expressed cell surface markers. …”

Image 9_Divergent B-cell and cytotoxic TNK cell activation signatures in HLA-B27-associated ankylosing spondylitis and acute anterior uveitis.jpeg by Eisa Mahyari (9681962)

“…Using cellular indexing of transcriptomes and epitopes (CITE-Seq) in a well-characterized cohort of 25 subjects—including AS (HLA-B27^pos), AS+AAU (HLA-B27^pos), AAU (HLA-B27^pos), HCs (HLA-B27^pos), and HCs (HLA-B27^neg); N = 5/group—we identified transcriptomic differences at the single-cell level, along with differentially expressed cell surface markers. …”

Table_1_Photosynthetic Carbon Fixation and Sucrose Metabolism Supplemented by Weighted Gene Co-expression Network Analysis in Response to Water Stress in Rice With Overlapping Grow... by Xinpeng Wang (4611307)

“…The results showed that short-term drought stress promoted photosynthetic carbon fixation. However, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) activity significantly decreased, resulting in a significant decrease in photosynthetic rate. …”

Data_Sheet_1_Diabetes Mellitus Is an Independent Risk Factor for a Stiff Left Atrial Physiology After Catheter Ablation for Atrial Fibrillation.PDF by Moon-Hyun Kim (12044069)

“…After 1:3 propensity score (PS) matching for age, sex, and AF type, we compared 211 patients with DM with 633 patients without DM. …”

Presentation_1_Small-Medium Extracellular Vesicles and Their miRNA Cargo in Retinal Health and Degeneration: Mediators of Homeostasis, and Vehicles for Targeted Gene Therapy.pdf by Yvette Wooff (4955854)

“…Isolated s-mEV from normal retinas were characterized using dynamic light scattering, transmission electron microscopy and western blotting, and quantified across 5 days of photo-oxidative damage-induced degeneration using nanotracking analysis. …”