PIAS1 inhibits the ability of TGFβto suppress E-cadherin promoter activity. by Stuart J. Netherton (152258)

“…Lysates of NMuMG cells transfected with the E-cadherin promoter driven luciferase (E-cadherin-p-luciferase) reporter and the β-galactosidase (β-gal) expression construct together with an empty expression vector (−), or one encoding wild type PIAS1 (WT) or SUMO E3 ligase mutant PIAS1 (CS), and left untreated or incubated with 100 pM TGFβ for 48 h, were subjected to luciferase and β-galactosidase assays (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013971#s4" target="_blank">MATERIALS and METHODS</a>). …”

FMNL3 mediates invasion of gonococcal colonies in macrophages. by Stanimir S. Ivanov (3394922)

“…<b>(D and E)</b> The Internalization index for each condition was calculated by dividing the percentage of internalized objects from infections of the knock out U937 MFs cell line by the percentage of internalized objects from infections of the parental U937 MFs, which was then multiplied by 100. Values > 100% indicate increased object internalization by the KO cell line compared to WT cells, whereas values < 100% indicate decreased object internalization by the KO cell line. …”

Effect of SRp20ΔRRM expression on poliovirus yield. by Kerry D. Fitzgerald (214008)

“…Cells expressing the deletion mutant, GFP-SRp20ΔRRM, displayed a two-log decrease in poliovirus yield (C). The observed decrease (∼100 fold) was consistent across three separate experiments, although overall titers for the GFP control between experiments ranged from ∼10⁵ to ∼10⁷ (likely due to the variability of DNA transfection efficiency). …”

The effects of the <i>p_yz</i> strength. by Yong-Jun Shin (184085)

“…<p>(A) As <i>p_yz</i> is decreased, periodic DNA repair-related fluctuation (<i>T</i> = 40 min and amplitude = 100 molecules/cell) is filtered less, so the p53 levels fluctuate more. …”

Synaptic potentiation reduces AP onset, threshold and AHPs. by Sung-Cherl Jung (261358)

“…B. Example traces upon +100 pA current injection before (black) and 30 min (red) after conditioning stimulations. …”

Intravenous injection of mAb c.8B6 does not induce allodynia. by Mickaël Terme (521478)

“…<p>(A) Intraveinous injection of either 1 mg/kg (•) or 3 mg/kg (♦)of mAb c.8B6 in Sprague-Dawley rats did not result in a decrease in mechanical thresholds. …”

OUTPATIENT ANALYTIC ASSESSMENT OF ANOREXIA NERVOSA — THE IMPORTANCE OF VENOUS BLOOD GASES by Sofia Alexandra Pereira Pires (6800096)

“…Respiratory acidosis was present in 91% in t1, 100% in t2 and 94% in t3. There was a significant decrease between t2 and t3 in mean pCO2 (57.2 versus 53.6 mmHg; p=0.009) and mean HCO3 (30.0 versus 28.8 mEq/L; p=0.023). …”

In vitro 14.7T MR T1-map images and the relative T1 values of SMCs incubated with MGd at various concentrations. by Han Wang (254423)

“…Measurement of T1 value further confirms the findings in <b>a</b>, demonstrating a linear decrease of T1 value from 0–100 ug/mL MGd, followed by a platform pattern of T1 values from 100–150 ug/mL MGd (b).…”

Examples of rtPCR Data Generated Using Quantified or Unquantified DNA from Specific ChIP Reactions. by Pamela D. Schoppee Bortz (199468)

“…The results illustrate three different scenarios affecting the interpretation of how PDGF-BB impacts <i>ACTA2</i> gene expression: in the first experiment (<b>A</b>) there was no effect of method (a 40% decrease in SRF interaction was detected with both methods); in the second experiment, using quantified DNA magnified the detected degree of change (a 92% versus a 50% decrease) in SRF interaction with the CArG-box region of the <i>ACTA2</i> promoter following PDGF-BB treatment (<b>B</b>) and actually reversed the results (<b>C</b>) and thus the interpretation of how PDGF-BB impacts histone 4 acetylation (a 74% decrease versus a 49% increase).…”

Analysis of ES clones by multiplex ligation-dependent probe amplification (MLPA). by Guo Ling Zhou (488153)

“…The relative peak areas of 5wt and 3wt were decreased by approximately half in target ES clones (4G02) but not in non-target ES clones (12F12). …”

Gemcitabine and ATR inhibitor synergism and effects on the cell cycle and DNA damage in U-CHCF365 and UM-Chor1 (Batch 1) by Chordoma Foundation (4391989)

“…</p><p><br></p><p dir="ltr">The following panels were treated according to panel A. The concentrations of gemcitabine and elimusertib used for U-CHCF365 were 5nM and 100nM, respectively, with those doses combined in the combination. …”

Gemcitabine and ATR inhibitor synergism and effects on the cell cycle and DNA damage in CH22 and U-CH17PII (Batch 2) by Chordoma Foundation (4391989)

“…</p><p><br></p><p dir="ltr">The following panels were treated according to panel A. The concentrations of gemcitabine and elimusertib used for CH22 were 5nM and 100nM, respectively, with those doses combined in the combination. …”

Sequential acceptor photobleaching data. by Heidi J. Chial (242879)

“…The percent decrease in YFP signal and corresponding FRET efficiency values for each of the 20 image sets were plotted and subjected to linear regression analysis to generate equations used to calculate FRET efficiency values when YFP is 100% bleached (lower graph).…”

Relative velocity in the venular network. by Bumseok Namgung (813359)

“…<p><b>A:</b> Typical example of the relative velocity decrease map at 30 mmHg. …”

Comparison of the fraction of positive relations for different behavioral updating. by Xiaochen He (455300)

“…<p>The simulation is carried out 10 times on a random network consisting of 100 nodes with average network connectivity 〈<i>k</i>〉 = 4. …”

Effects of bending stiffnesses of ACP^C, <i>κ</i>_b,ACP,1 and <i>κ</i>_b,ACP,2, on <i>G</i>′ and <i>G</i>″. by Taeyoon Kim (122486)

“…<i>κ</i>_b,ACP = control (black circles), 10-fold decrease (magenta triangles), 100-fold decrease (blue inverted triangles), and 0 (green diamonds) (A) <i>γ</i> = 0, (B) <i>γ</i> = 0.4, (C) <i>γ</i> = 0.55. …”

The metabotropic receptors linked to PLC-β4 in the LGNd were analyzed using Ca²⁺ imaging techniques. by Masayuki Ikeda (252072)

“…<p>A. Orexin-A (300 nM) and orexin-B (300 nM) failed to mobilize intracellular Ca²⁺ in the LGNd of wild-type mice. …”

Dose-dependent binding of the ficolins (0.2 µg) to microorganisms. by Tina Hummelshøj (237744)

“…OD450nm values at 100% for the ficolin-A, ficolin-2 and ficolin-3 assays were 1.263, 2.561 and 1.338, respectively. …”

Persulfidation modulates the activity of <i>A</i>. <i>fumigatus</i> virulence–associated proteins and is important for its pathogenic potential. by Monica Sueiro-Olivares (5785451)

“…<p><b>(a)</b> Representative images of in-gel detection of persulfidation levels in whole protein extracts of <i>A</i>. <i>fumigatus</i> wt and mutants <i>ΔmecB</i> (CTH), <i>ΔmecA</i> (CBS), and <i>ΔmstA</i> (MST). …”

Table_6_Depletion of tet2 results in age-dependent changes in DNA methylation and gene expression in a zebrafish model of myelodysplastic syndrome.xlsx by Yaseswini Neelamraju (4509259)

“…The gene encoding ten-eleven translocation 2 (tet2), a dioxygenase enzyme that catalyzes the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine, is a recurrently mutated tumor suppressor gene in MDS and other myeloid malignancies. …”