The conserved motifs in the SBD of PluR and PauR are essential but not sufficient for ligand-binding specificity. by Sophie Brameyer (731567)

“…(<b>B</b>) and (<b>D</b>): The RLU values are depicted 2 h after the addition of the distinct signaling molecules, either 3.5 nM PPYD, 3.5 nM DAR or 100 nM C8-HSL. Reference line was set to 370 RLUs to compare better with <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124093#pone.0124093.g002" target="_blank">Fig 2</a>.…”

MIA/CD-RAP activates the <i>p54^nrb</i> promoter via the transcription factor YBX1. by Rainer Schmid (496848)

“…<p>(<b>A</b>) YBX1 activity as measured using a reporter gene assay revealed a significant decrease in YBX1 promoter activity in MIA-/- mMSCs compared with WT mMSCs (III) (**p<0.01). …”

Abberant α-Synuclein Confers Toxicity to Neurons in Part through Inhibition of Chaperone-Mediated Autophagy by Maria Xilouri (264240)

“…</p><h3>Methods and Findings</h3><p>Novel inducible SH-SY5Y and PC12 cell lines expressing human WT and A53T ASYN, as well as two mutant forms that lack the CMA-targeting motif were generated. …”

CV1720 cells show impaired ATR–dependent DNA damage responses. by Tomoo Ogi (122258)

“…<p>A) WT, DK0064 (ATR–SS), CV1720 (patient), CV1780 (patient's mother) and CV1783 (patient's father) cells were exposed to 5 Jm⁻² UV and the mitotic index (MI) assessed 2 h post exposure. …”

Proliferation of CTS-treated cells. by Rebecca J. Clifford (497197)

“…<p>A) Normal MCF10A cells exhibited a concentration-dependent decrease in BrdU incorporation (proliferation) when treated with 500 nM ouabain, 50 nM bufalin or 100 nM bufalin for 2 hours (p<0.01). …”

LPS triggered chemotactic signaling through fAR1. by Miao Pan (5285834)

“…A Student <i>t</i> test indicated a statistically significant difference in LPS binding between <i>far1</i>^<i>−</i> and <i>WT</i> cells (* indicates <i>P</i> < 0.01). …”

Optimizing light-cured composite through variations in camphorquinone and butylhydroxytoluene concentrations by Hani NASSAR (12805999)

“…Sixteen groups of resin composite were prepared using combinations of four CQ (0.1%, 0.5%, 1.0%, and 1.5%) and four BHT (0.0%, 0.5%, 1.0%, and 1.5%) concentrations. …”

Loss of Slfn3 induces a gender-dependent repair vulnerability after 50% bowel resection by Emilie DeKrey (9389738)

“…Mitotic marker, Phh3+, and proliferation marker expression of Lgr5, FoxL1, and PDGFRα were increased after resection in male WT but this effect was blunted in male Slfn3KO mice. …”

Effect of inhibitors of p38 MAPK or PKC on stimulated shedding by ADAM17T735A and ADAM17ΔC. by Katherine C. Hall (180988)

“…C) <i>Adam17−/−</i> mEFs transfected with TGFα together with WT ADAM17 were stimulated with 5 ng/ml IL-1β, 1 µM anisomycin or 25 ng/ml PMA in the presence or absence of 4 µM BIM I. …”

Myelin alteration in 8-week-old TRAF4-KO mice. by Sébastien Blaise (332585)

“…(C): Western blot analysis of the myelin-specific MBP marker and of the interglial-specific TJ marker claudin 11 (cl 11) showed a decreased expression level of both proteins in 4 independent TRAF4-KO cerebellum protein extracts (lanes 5–8) compared with 4 WT samples (lanes 1–4) ; tubulin served as a loading control. …”

Expression of two <i>tenascin</i> genes is affected by temperature. by Che-Yi Lin (218292)

“…<p><i>In situ</i> hybridization against <i>tenascin-c</i> (<i>tnc</i>) (A-H) and <i>tenascin-w</i> (<i>tnw</i>) (L-O) in 22-ss wild-type (WT) and <i>s1pr2^as10</i> (<i>as10</i>) mutant embryos raised at 28.5°C or 22.5°C. …”

Supplemental data of the manuscript “Accumulation of seminolipid in Sertoli cells is associated with increased levels of reactive oxygen species and male subfertility: Studies in a... by Kessiri Kongmanas (7467368)

“…</p> <p><b>Supplemental Figure S7</b> Levels superoxide anions (2HE) and other ROS + oxidants (Eth) of the cauda epididymis of 5-month-old <i>Arsa^-/-</i> mice and WT mice<b> (A)</b> and of the caput epididymis of 5-month-old and 8-month-old <i>Arsa^-/-</i> mice and age-matched WT mice <b>(B)</b>. …”

Thermally conductive polyethylene/expanded graphite composites as heat transfer surface: Mechanical, thermo-physical and surface behavior by Sobolčiak, Patrik

“…Moreover, the surface wettability of the HDPE/EG composite was enhanced by corona and RF plasma treatment as demonstrated by the increase in surface free energy from 28.5 mJ/m2 for untreated HDPE/EG to 55.5 and 54.5 mJ/m2 for HDPE/EG treated by corona and RF plasma, respectively. …”
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Rac2 promotes differentiation of M2 macrophages <i>in vitro</i>. by Shweta Joshi (555748)

“…(D) & (E), Heatmap representation of metabolites across BMDMs from WT (n = 5) and Rac2-/- (n = 5) mice. Shades of yellow represent elevation of a metabolite and shades of blue represent decrease of a metabolite relative to the median metabolite levels (see color scale). …”

SLS-4 is required for the <i>in vitro</i> ubiquitination of poly-SUMO-2 chains and degradation of SUMO conjugates <i>in vivo</i>. by Chris Boutell (205885)

“…<p>(A) <i>In vitro</i> ubiquitination reactions were carried out in the presence of E1, UbcH5a and ubiquitin (Ubmix), poly-SUMO-2 chains and purified wt ICP0, ICP0ΔRING, or ICP0 C-terminal truncation mutants ICP01-323, ICP01-396 or ICP01-396mSLS-4 as indicated (-/+). …”

Table_1.pdf by Hena Ahmad (4995290)

“…Both, western blot and confocal microscopy confirmed an increase of ataxin-2 in BE(2)-M17 cells transduced with lentivirus expressing mt-ELF2 (CEE-mt-ELF2), which was not observed in cells transduced with lentivirus expressing wt-ELF2 (CEE-wt-ELF2). Moreover, we observed a significant decrease in the number and size of lipid droplets in the CEE-mt-ELF2-transduced BE (2)-M17 cells, but not in the CEE-wt-ELF2-transduced BE (2)-M17. …”

Image_1.pdf by Hena Ahmad (4995290)

“…Both, western blot and confocal microscopy confirmed an increase of ataxin-2 in BE(2)-M17 cells transduced with lentivirus expressing mt-ELF2 (CEE-mt-ELF2), which was not observed in cells transduced with lentivirus expressing wt-ELF2 (CEE-wt-ELF2). Moreover, we observed a significant decrease in the number and size of lipid droplets in the CEE-mt-ELF2-transduced BE (2)-M17 cells, but not in the CEE-wt-ELF2-transduced BE (2)-M17. …”

<i>Mmp13</i>^−/− mice display increased bone volume during non-stabilized fracture healing. by Danielle J. Behonick (81485)

“…Scale bar = 1 mm (B) Histomorphometric measurements of total bone volume (BV) and total bone volume as a proportion of total callus volume (BV/TV) in WT and <i>Mmp13</i>^−/− mice at days 7 (WT <i>n</i> = 6, <i>Mmp13</i>^−/−<i>n</i> = 6), 10 (WT <i>n</i> = 8, <i>Mmp13</i>^−/−<i>n</i> = 6), 14 (WT <i>n</i> = 8, <i>Mmp13</i>^−/−<i>n</i> = 6), 21 (WT <i>n</i> = 6, <i>Mmp13</i>^−/−<i>n</i> = 6), 28 (WT <i>n</i> = 6, <i>Mmp13</i>^−/−<i>n</i> = 6) and 56 (WT <i>n</i> = 5, <i>Mmp13</i>^−/−<i>n</i> = 4) confirm this observation There is a statistically significant decrease in BV in <i>Mmp13</i>^−/− calluses compared with WT at day 7 (*p<0.05), but a statistically significant increase in BV and BV/TV in <i>Mmp13</i>^−/− calluses compared with WT at days 28 (**p<0.01 and p<0.05 respectively) and 56 (*p<0.05). …”

Rho-mediated control of <i>kinB</i> transcription is removed during sporulation. by Vladimir Bidnenko (4258573)

“…The highlighted points correspond to the WT profiles shown in panel (A): LBtran (orange), S1 (green), S5 (blue), S6 (pink); and RM (red) and WT (black) during exponential growth in LB. …”

Representation of the GE356 Fab crystal structure and paratope mapping analysis. by Marjon Navis (92808)

“…The color-coding reflects fold-decrease in binding or of neutralization activity as compared to WT, in red (>50× WT), dark orange (20–50× WT), light orange (10–20× WT) and yellow (5–10× WT). …”