Verification of the knock out efficiency of <i>VPREB1</i> gene in myeloma cells by immunofluorescence. by Mai Khaled (9962968)

“…There was no significant difference (p>0.05) in VPREB1 protein expression between the 1^st and the 2^nd transfection approaches. The (a, b and c) are 100X magnification and the (d, e and f) are 400X magnification. …”

Stability of trimers after fixation in selected conditions. by Yu Feng (1234077)

“…Unstable (–) is defined as < 30% relative binding of PGT145 and VRC01 with increased F105 binding coupled with a marked decrease (<30% remaining) of the trimer band by BN-PAGE.…”

FCF affects HIF-1α expression on the posttranslational level. by Dikla Vardi-Oknin (447319)

“…<p>(<b>A</b>) PC-3 cells were pretreated with 100 μM FCF for 2 h and then subjected overnight to normoxic or hypoxic conditions. …”

Data Sheet 3_Global burden and projections of stroke and its subtypes attributable to high alcohol use during 1990–2021: insights from the global burden of disease study 2021.docx... by Fangfang Zhan (12129294)

“…By 2030, the ASMR for high alcohol use-attributable stroke is projected to decline from 4.3 per 100,000 in 2021 to 4.15 per 100,000, while the ASDR is forecast to decrease from 98 per 100,000 in 2021 to 95 per 100,000.…”

Data Sheet 2_Global burden and projections of stroke and its subtypes attributable to high alcohol use during 1990–2021: insights from the global burden of disease study 2021.docx... by Fangfang Zhan (12129294)

“…By 2030, the ASMR for high alcohol use-attributable stroke is projected to decline from 4.3 per 100,000 in 2021 to 4.15 per 100,000, while the ASDR is forecast to decrease from 98 per 100,000 in 2021 to 95 per 100,000.…”

Data Sheet 1_Global burden and projections of stroke and its subtypes attributable to high alcohol use during 1990–2021: insights from the global burden of disease study 2021.docx... by Fangfang Zhan (12129294)

“…By 2030, the ASMR for high alcohol use-attributable stroke is projected to decline from 4.3 per 100,000 in 2021 to 4.15 per 100,000, while the ASDR is forecast to decrease from 98 per 100,000 in 2021 to 95 per 100,000.…”

DEP dissociate VEGF-R2 from adherens junction connection. by Chia-Yi Tseng (765451)

“…<p>(A) After 24 h DEP (1, 10 and 100 g/mL) exposure, the tube cells were lysed and harvested. …”

Data Sheet 4_Global burden and projections of stroke and its subtypes attributable to high alcohol use during 1990–2021: insights from the global burden of disease study 2021.docx... by Fangfang Zhan (12129294)

“…By 2030, the ASMR for high alcohol use-attributable stroke is projected to decline from 4.3 per 100,000 in 2021 to 4.15 per 100,000, while the ASDR is forecast to decrease from 98 per 100,000 in 2021 to 95 per 100,000.…”

the UNC-44/UNC-33 pathway regulates gap junction dynamics and turnover. by Lingfeng Meng (2576632)

“…<p><b>(A)</b> Time kymographs generated from a 30s movie of GFP::UNC-9 dynamics in control, <i>unc-33(lf)</i> and <i>unc-44(lf)</i> animals. …”

PKC signaling and calcineurin independently regulate tolerance to ergosterol biosynthesis inhibitors via a common target in <i>C. albicans</i>. by Shantelle L. LaFayette (243114)

“…(<b>B</b>) Simultaneous inhibition of calcineurin and Pkc1 signaling does not synergistically decrease FL tolerance of a WT strain (SN95). A fractional inhibitory concentration (FIC) assay was carried out in YPD medium containing a fixed concentration of 0.5 µg/mL FL and gradients of the calcineurin inhibitor cyclosporin A (CsA) and the PKC inhibitor staurosporine (STS). …”

Signal transduction. by Karen V. Kibler (230157)

“…For this CBA analysis, PBMCs were mock-infected or infected at an MOI of 5 with NYVAC wt, NYVAC-C, NYVAC-C-KC, NYVAC-C-ΔB19R or NYVAC-C-KC-ΔB19R. …”

Data_Sheet_1_Improving the Lifetime of CsPbBr3 Perovskite in Water Using Self-Healing and Transparent Elastic Polymer Matrix.docx by Livy Laysandra (9462983)

“…The tensile strength and stretching ability of 5 wt% perovskite content in the random copolymer were observed to be 3.8 MPa and 553.5% respectively. …”

Table1_Proteomic study of medicinal mushroom extracts reveals antitumor mechanisms in an advanced colon cancer animal model via ribosomal biogenesis, translation, and metabolic pat... by Boris Jakopovic (9265517)

“…</p>Methods<p>In this study, we performed both qualitative and quantitative proteomic analyses using tandem mass tags (TMT) on CT26 wild type (CT26. WT) colon cancer tissues from Balb/c mice, which were treated with a special blend of medicinal mushroom extracts, either alone or in combination with the chemotherapy drug 5-fluorouracil.…”

DataSheet1_Proteomic study of medicinal mushroom extracts reveals antitumor mechanisms in an advanced colon cancer animal model via ribosomal biogenesis, translation, and metabolic... by Boris Jakopovic (9265517)

“…</p>Methods<p>In this study, we performed both qualitative and quantitative proteomic analyses using tandem mass tags (TMT) on CT26 wild type (CT26. WT) colon cancer tissues from Balb/c mice, which were treated with a special blend of medicinal mushroom extracts, either alone or in combination with the chemotherapy drug 5-fluorouracil.…”

Loss of <i>Fgf3</i> causes variable anterior-posterior axis truncation and caudal malformation. by Matthew J. Anderson (265883)

“…<i>Fgf3</i> mRNA is detected in the primitive streak (<b>ps</b>, E7.5, E8.5) as well as in the presomitic mesoderm (E8.5–12.5) and tail bud (E9.5–12.5). …”

SP causes morphological changes, intracellular calcium increases and impedance changes in U373MG cells. by John Meshki (36999)

“…<p>(<b>A</b>) Phase-contrast images of U373MG cells treated with SP (100 nM). …”

BV modulates GSK3β-mediated suppression of Foxp3⁺ Treg cells <i>in vitro</i>. by Ran Fu (434520)

“…(B), Both BV (100 μg/mL) and GSK3β siRNA (100 pmol/mL) were shown to significantly increase the frequencies of Foxp3+ Treg cells in cultured splenocytes. …”

Abnormal development of neural stem cell-derived neurospheres from <i>Raldh2^−/−</i> spinal cords. by Marie Paschaki (179823)

“…<p>Neurospheres were derived from dorsal cervical/brachial spinal cord from E12.5 WT and <i>Raldh2^−/−</i> embryos. (A,B) After growth of dissociated progenitor cells in suspension, in 6-well plates, for 10 days in a defined selective medium (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032447#s4" target="_blank">Materials and Methods</a>), the number of spheres developing from <i>Raldh2^−/−</i> embryos spinal cords is decreased by 25% compared to WT (E: 11.1±0.88 spheres/well for WT; 8.40±1.50 for mutants; n = 9; T- test: P value = 0,0009). …”

<i>Sim1</i> loss-of-function <i>in vitro</i> and <i>in vivo</i>. by Nadja Osterberg (352748)

“…B-M: In situ hybridization for <i>Pet1</i> (B-E), <i>RGS4</i> (F-I) and <i>Brn3.2</i> (J-M) in wild type (<i>wt</i>) and <i>Sim1-/-</i> mutants at P0. <i>Pet1</i> expression was observed in the area of rostral 5-HT neurons (B) including the area of dorsal raphe in <i>wt</i> (C: magnification of the inset in B), but was considerably decreased in <i>Sim1-/-</i> (D). …”

Knockout animals are less persistent and less consistent in their drinking behavior. by Admir Resulaj (12368645)

“…(D) This plot controls for the possibility that KO animals have a higher lick rate than WT and thus fatigue more quickly. KO and WT animals perform, on average, the same number of licks per trial (mean ± std: 23.2 ± 9.9 CD/trials for KO versus 29.4 ± 5.8 CDs/trial for WT; p > 0.17, two-sided t-test). …”