Validation of target gene knockdown at mRNA and protein levels. by Rajshekhar Y. Gaji (417441)

“…<p>A. The percent decrease in mRNA levels of target genes transfected with gene-specific siRNA were determined using q-RT PCR 72 h post-transfection. …”

Immmocytochemistry of DMT1−IRE, DMT1+IRE, Fpn1 and TfR1 proteins in cortical neurons. by Fang Du (232423)

“…<p>Cells were treated with L-DOPA (0, 1, 5, 10, 100, 200 µM) in DMEM+5%FBS for 16 hours and then immunostained for DMT1−IRE (A), DMT1+IRE (B), Fpn1 (C) or TfR1 (D) antibodies. …”

File S1 - A Genetically Encoded Reporter for Real-Time Imaging of Cofilin-Actin Rods in Living Neurons by Jianjie Mi (504427)

“…</b> The affinity of cofilinR21Q for F-actin is decreased substantially below that of wt or cofilinR22Q as measured by F-actin sedimentation. …”

Positive feedback in <i>CLN2</i> activation and the coherence of entry into Start. by Debashis Barik (3389255)

“…</b> As the rate constant for phosphorylation of Whi5 by Cln1,2 (i.e., the strength of the Cln1,2-mediated positive feedback loop) is progressively decreased (in panels C1-C5), the coherence of gene activation upon entry into Start decreases. …”

Image 2_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Table 1_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Image 1_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

Table 2_Long-term trends and comparison of the burden of lower respiratory tract infections in China and globally from 1990 to 2021: an analysis based on the Global Burden of Disea... by Zhiwei Wang (120259)

“…Compared with the previous period, the COVID-19 pandemic has led to a significant decrease in the disease burden of LRIs. …”

File S1 - Novel NAC Transcription Factor TaNAC67 Confers Enhanced Multi-Abiotic Stress Tolerances in <i>Arabidopsis</i> by Xinguo Mao (233950)

“…Four replications were performed, and values are means ± SE. Fig. S4, A rapid decrease, slow increase and slow decrease pattern in chlorophyll content was identified Arabidopsis after exposure to high salinity. …”

Fitness and distribution of cell types. by Joshua R. Christie (725052)

“…The decrease in heteroplasmy in <i>B</i>₁<i>B</i>₂ cells between generations 0–100 is an artifact of introducing <i>U</i>₁ at a frequency of 0.01 (the influx of <i>U</i>₁ gametes homoplasmic for the wild type haplotype converts some heteroplasmic <i>B</i>₁ and <i>B</i>₂ gametes into homoplasmic gametes, which increases the proportion of homoplasmic <i>B</i>₁<i>B</i>₂ cells). …”

Interactions between active and inactive (binding-defective) β₂ARs during internalization. by Tien-Hung Lan (229446)

“…No detectable internalization of Flag-β₂AR D113S was promoted by unlabeled wt β₂AR. <b>C</b>, decreases in BRET (<i>left</i>) and loss of cell surface Flag immunoreactivity (<i>right</i>) after isoproterenol treatment in cells expressing the indicated combinations of β₂AR-Rluc8 and Flag-β₂AR (<i>n</i> = 4–5). *, <i>P</i><0.05; **, <i>P</i><0.005, paired t-test, meaning ΔBRET or % internalization is significantly different from zero. …”

Investigating Axonal Dysfunction in Alzheimer’s Disease: A Live Cell Imaging Approach by Jiyeon Song (5571476)

Supplementary Material for: Could a Possible Crosstalk between AMPK and TGF-β Signaling Pathways Be a Key Player in Benign and Malignant Salivary Gland Tumors? by Ghahhari N.M. (4136137)

Female protection against diabetic kidney disease is regulated by kidney-specific AMPK activity by Hak Joo Lee (18423230)

TSS-targeted siRNA abolishes the heparanase expression in cancer cells. by Guosong Jiang (182435)

“…The symbols (# and Δ) indicate a significant decrease from untransfected control (mock) and a significant increase from pGL3/Basic, respectively.…”

Met-depleted mosquitoes exhibit metabolic defects. by Yuan Hou (767606)

“…<p>(A) A decrease in the glycogen levels was observed in Met-depleted adult female mosquitoes, as measured by colorimetry (<i>left</i>) and PAS staining (<i>right</i>). …”

Fis binding site modifications, Langevin dynamics simulations of DNA breathing, and EMSA experiments, the first set. by Kristy Nowak-Lovato (101951)

“…<p>(A) Langevin dynamics simulations demonstrating suppressed local DNA breathing dynamics in the FIS1S sequence (compare to FIS1 in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002881#pcbi-1002881-g002" target="_blank">Figure 2</a>). …”

Systematic review of evidence for the impact and effectiveness of the 1-3-7 strategy for malaria elimination by Richard J. Maude (9416228)

“…</p><h2>Conclusion </h2><p dir="ltr">Overall, the 1-3-7 approach was effectively implemented with a concomitant decrease in cases in malaria elimination settings, however, it was not possible to quantify impact as it was not implemented in isolation. …”

<i>PIK3CA</i>^<i>mut</i> potentiate proliferation and migration <i>in vitro</i>. by Robert S. McNeill (5485064)

“…<p>MTS assays showed that <i>PIK3CA</i>^<i>WT</i> and all <i>PIK3CA</i>^<i>mut</i> decreased doubling times of NHA (<b>A</b>), but not NHA^RAS (<b>B</b>) (*, P≤0.02 vs parental, <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0200014#pone.0200014.s005" target="_blank">S5A and S5B Fig</a></b>). …”

DataSheet_1_Disruption of Glucocorticoid Action on CD11c+ Dendritic Cells Favors the Generation of CD4+ Regulatory T Cells and Improves Fetal Development in Mice.pdf by Lianghui Diao (9420926)

“…Reproductive outcome and maternal immune and endocrine adaptation of Balb/c-mated C57Bl/6 GR^flox/floxCD11c^cre/wt (mutant) females was assessed on gestation days (gd) 13.5 and 18.5. …”