Real-time PCR quantifications in the presence of primers with different annealing abilities. by Michele Guescini (169183)

“…Each symbol represents a mean of 4 runs. SOD detected all the runs obtained using [ND1F5]≥250 nM as outliers.…”

Ischemia depresses the amplitude of evoked synaptic transmission without changes in the presynaptic volley amplitude. by Shirin Jalini (2551054)

“…<p>(A). fEPSPs were recorded from the stratum radiatum in the CA1 region while the Schaffer collaterals were stimulated every 15 s. fEPEP amplitude decreased reversibly in a time-dependent manner after 2 min (n = 7), 4 min (n = 5), and 6 min (n = 5) of OGD. …”

Involvement of clathrin-mediated endocytosis in NTBI uptake by PBMCs and HepG2 cells. by Joao Arezes (488491)

“…<p>(A–B) ⁵⁵Fe uptake by PBMCs and HepG2 cells in the presence of 0.45M of sucrose (A) or 80 µM of Dynasore (B) to inhibit clathrin-mediated endocytosis, after incubation with 5 µM of ⁵⁵Fe-citrate (5∶100) at 37°C (C–D) ¹²⁵I-Transferrin internalization in the presence of 0.45M of sucrose (C) or 80 µM of Dynasore (D). …”

MSR algorithm analysis. by Federico Comoglio (224744)

“…(B) The mean move length significantly decreases as a function of the protein length. …”

Collapse regime, hysteresis, and recovery of plant–pollinator mutualistic networks. by Gaurav Baruah (7257587)

“…(B, C) As the transition parameter, which is <i>γ</i>₀, decreases, the mutualistic network transitions from a high-functioning stable state—high species richness in B and high biomass in C—to a low-functioning undesirable state—low richness in B and low biomass depicted by dark pink points. …”

Vulnerability to high fat diet-induced obesity. by Alessandro Bartolomucci (165364)

“…At baseline, when mice were fed standard diet, all experimental groups showed a decrease in body weight, while controls (Con) showed a slight increase (F(3,23) = 3.2, p<0.05). …”

Selective Enrichment of Cysteine-Containing Phosphopeptides for Subphosphoproteome Analysis by Mingming Dong (1471396)

“…Interestingly, amino acid residues surrounding the identified phosphosites were enriched with buried residues (L, V, A, C) while depleted with exposed residues (D, E, R, K). …”

Measurement of mitochondrial function in nasal fibroblasts with and without TGF-β1 or with and without PX-478 To investigate whether mitochondrial function are elevated by TGF-β1,... by Min-Sik Jo (14636809)

“…<p>Oxygen consumption rate (OCR) was significantly increased at 5 ng/ml TGF-β1 (S1A Fig). To determined whether PX-478 decrease mitochondrial function, PX-478 (20 nM) was pretreated for 1 hour, followed by TGF-β1 48 hours. …”

DataSheet_2_Molecular characterization of cross-kingdom RNA interference in Botrytis cinerea by tomato small RNAs.xlsx by Si Qin (3627863)

“…In order to study the functionality of this plant sRNA in reducing the Bcspl1 transcript level, we generated a fungal mutant that contained a 5-nucleotide substitution that would abolish the interaction between the transcript and the sRNA without changing the encoded protein sequence. …”

Modelling of the effects of En1 and En2 on hippocampal cells. by Asma Soltani (4356841)

“…In normal conditions (black lines), low levels of endogenous secreted En1 (from GABA cell for instance) could participate to dendritogenesis (1), spinogenesis (2) (insert), and mTOR signaling (3) in neighbor glutamatergic neurons. In a pathological context, such as autism (red lines), a rise of En2 expression and secretion would generate additional effects, i.e. the increase in dendritic complexity of GABA cells (4), a decrease of clustered PSD95 (5), and the mispairing of glutamatergic synapses (6) (insert). …”

cGMP-Dependent Protein Kinase Type I Is Implicated in the Regulation of the Timing and Quality of Sleep and Wakefulness by Sonja Langmesser (57421)

“…Moreover, PRKG1 deficiency results in an aberrant pattern, and consequently a reduced quality, of sleep and wakefulness, possibly due to a decreased wake-promoting output of the circadian system impinging upon sleep.…”

Altered Cerebrospinal Fluid Proteins in Smith–Lemli–Opitz Syndrome by Wenping Li (440398)

Olfactory Bulb Glomerular NMDA Receptors Mediate Olfactory Nerve Potentiation and Odor Preference Learning in the Neonate Rat by Rebecca Lethbridge (173780)

“…<em>Ex vivo</em> experiments using bulbs from trained rat pups reveal an increase in the AMPA/NMDA EPSC ratio post-training, consistent with an increase in AMPA receptor insertion and/or the decrease in NMDAR subunits. These results support a model of a cAMP/NMDA interaction in generating rat pup odor preference learning.…”

Use of 2-Aminopurine Fluorescence To Examine Conformational Changes during Nucleotide Incorporation by DNA Polymerase I (Klenow Fragment)^† by Vandana Purohit (2947848)

“…Finally, when the primer had a 3‘-OH, a fluorescence decrease with a rate equal to the rate of nucleotide incorporation was observed with both 0 and +1 position reporters. …”

An immunohistochemically stained photomicrograph of the stomach mucosa using an anti-TLR4 antibody. by Rehab F. Taher (20478600)

“…<p>(a) few TLR4-positively stained cells in the normal group; (b) the control ethanol group showed elevated TLR4 expression and a higher proportion of positive cells having deep brown coloring, (c) Omeprazole group showed reduction in the percentage of TLR4 positive cells with strong brown coloring, (d) PM-EtOH (25 mg/kg) group showed lowered percentage of TLR4 positive cells with weak cytoplasmic staining, (e) PM-EtOH (5o mg/kg) group showed decreased percentage of TLR4 positive cells, (f) PM-EtOH (100 mg/kg) group giving weak TLR4 expression, with a significant decrease in TLR4-positively stained cells(TLR4 immunohistochemical staining; Scale bar=100µm).…”

An Inducible Cell-Cell Fusion System with Integrated Ability to Measure the Efficiency and Specificity of HIV-1 Entry Inhibitors by Alon Herschhorn (198447)

“…Target cells express the HIV-1 receptors, CD4 and CCR5, and carry the firefly luciferase (F-Luc) reporter gene under the control of a tTA-responsive promoter. …”

Altered Cerebrospinal Fluid Proteins in Smith–Lemli–Opitz Syndrome by Wenping Li (440398)

Representative kinetic traces illustrating the JRA-associated abnormality in metabolic oscillations of neutrophils by James N Jarvis (3342)

“…However, in contrast to MPO-negative cells, MPO-positive cells undergo both a decrease in period to 10 seconds and a dramatic increase in the oscillatory amplitude. …”

Changes in Expression Levels of miR-1 and miR-133 and their targets in canine HF Myocytes. by Andriy E. Belevych (338034)

“…*p<0.05, n = 5. C. Representative Western blots of putative targets of miR-1 and -133, regulatory subunits B56α, B56δ and catalytic subunit of PP2A respectively. …”

Lab 3: Biol2050 Abundance and Diversity in Insects Data Set 1 by Katrin Chapkis (797943)

“…When the wind speed was found in the Beaufort scale, it was given a Beaufort number of 5, which corresponded to Fresh Breeze in Seaman's term.…”