Safe and effective subcutaneous adipolysis in minipigs by a collagenase derivative by Fuding Chen (8207553)

“…Three vectors were constructed for expressing rColH(FM, mutant for fat melting, with 6xHis tag), rColH(WT, wild-type, with 6xHis tag), and rColH(E451D, E451D mutant, without 6xHis tag) in <i>Escherichia coli</i>. rColH(FM) & rColH(WT) were purified by Ni Sepharose on a laboratory scale, while rColH(E451D) was purified by five chromatography purification steps on a large scale. …”

Spontaneous EPSCs and IPSCs in wildtype and <i>orexin/ataxin-3</i> mice. by Natsuko Tsujino (169784)

“…Inset shows mean sEPSC amplitude of wildtype mice (WT) and <i>orexin/ataxin-3</i> mice (Tg). C. Cumulative probability plots of sIPSC inter-event intervals (n = 6–7), showing decrease in sIPSC frequency in <i>orexin/ataxin-3</i> mice. …”

A genome-wide genetic screen identifies modifiers of LRRK2 toxicity in yeast. by Yulan Xiong (250746)

“…<p>(A) Yeast gene deletion strains <i>ahc2Δ</i> and <i>rmd8Δ</i> markedly enhance LRRK2-induced toxicity in yeast compared to the WT strain. Yeast WT strain BY4741 and gene deletion strains <i>ahc2Δ</i> and <i>rmd8Δ</i> cells were transformed with galactose-inducible expression constructs containing the central GTP-COR-Kin fragment of WT LRRK2 or empty vector as a control. …”

RESPONSES OF PLASMA ADIPOKINES TO HIGH INTENSITY INTERVAL TRAINING: SYSTEMATIC REVIEW by Geovani Messias da Silva (10403182)

Study on the properties of oil-water interface after the interaction between ionized water and naphthenic acid by Qin Guowei (22155073)

“…The results show that after the action, the interfacial tension of oil and water continues to decrease, and 1/3 ionized water (salinity diluted to 1/3 of the prepared ionized water, 1253 ppm) decreases to a maximum of 50.89%, while 1/5 ionized water (752 ppm) only decreases 14.40%; the thickness of the oil-water interface film decreases with the salinity of ionized water, but the decrease increases after 1/3 ionized water. …”

Examples of rtPCR Data Generated Using Quantified or Unquantified DNA from Specific ChIP Reactions. by Pamela D. Schoppee Bortz (199468)

“…The results illustrate three different scenarios affecting the interpretation of how PDGF-BB impacts <i>ACTA2</i> gene expression: in the first experiment (<b>A</b>) there was no effect of method (a 40% decrease in SRF interaction was detected with both methods); in the second experiment, using quantified DNA magnified the detected degree of change (a 92% versus a 50% decrease) in SRF interaction with the CArG-box region of the <i>ACTA2</i> promoter following PDGF-BB treatment (<b>B</b>) and actually reversed the results (<b>C</b>) and thus the interpretation of how PDGF-BB impacts histone 4 acetylation (a 74% decrease versus a 49% increase).…”

Mitochondrial function. by J. Anthony Graves (164485)

Microstructural evolution of aluminum-A356 alloy with and without SiC reinforcement submitted to mechanical milling by Stella Ordoñez (5551250)

Changes in cell adhesion and in neuronal morphology induced by sAPPα, sAPPβ and sAPP-Fc added to neurons after 1 DIV. by Stéphanie Chasseigneaux (231744)

“…(<b>C–E</b>) Morphometric analysis 96 hours after the addition of sAPPs (50 to 150 nM) to 1DIV primary neurons. (C) At 50 and 100 nM sAPPα and sAPPβ induce a significant decrease of primary dendrite length while at 150 nM, the decrease in primary dendrite length is still significant for sAPPβ only (50 nM: control (Ctrl) vs sAPPα: p<0.0001, control vs sAPPβ: p<0.0001; 100 nM: control vs sAPPα: p<0.0001, control vs sAPPβ: p<0.0001; 150nM: control vs sAPPα: NS, control vs sAPP-Fc: NS, sAPPα vs sAPP-Fc: NS, control vs sAPPβ: p<0.0001). …”

Inhibition of cellular proliferation and induction of cell cycle arrest by PEG-8000 in SCC-25 cells- SCC-25 cells were treated with different concentrations of PEG-8000 for 24 h an... by Ramesh K. Wali (305603)

“…<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0038047#pone-0038047-g004" target="_blank">Figure 4B</a> shows a ∼50% decrease on the expression of proliferation marker PCNA by PEG. …”

Data_Sheet_1_The Acetyltransferase RibT From Bacillus subtilis Affects in vivo Dynamics of the Multimeric Heavy Riboflavin Synthase Complex.pdf by Daniel Andreas Orlando Rotter (12417451)

“…Complementarily, either deletion of ribT or mutation of a key residue from RibH (K29) suspected to be the substrate of RibT for acetylation leads to increased levels of subpopulations considered as capsids of RibH-mVenus (RibH-mV) in comparison to wild-type (wt)-like cells. …”

Dlar is localized to the larval muscle membrane at Z-discs. by Jessica Kawakami (12667170)

“…Ubiquitous RNAi knockdown by da-Gal4 results in a decrease in Dlar protein levels (right panel). Bar graph shows the relative levels of Dlar/ATP5α in control and Dlar RNAi larvae. …”

Data Sheet 1_Concurrent training and intermittent fasting reduce transverse cross-sectional area of cardiomyocytes and body mass in Wistar rats.pdf by Henrique Izaias Marcelo (21810809)

“…</p>Conclusion<p>The practice of concurrent training for a period of 4 weeks precipitated a decrease in the transverse cross-sectional area of the cardiomyocytes of Wistar rats. …”

EVALUATION OF POLY(VINYL ALCOHOL) ADDITION EFFECT ON NANOFIBRILLATED CELLULOSE FILMS CHARACTERISTICS by Eliane Lopes da Silva (5695766)

“…The thermal and mechanical properties of the obtained films were evaluated. Low levels (2, 5 and 10 wt%) of PVA were used as an additive in a nanofibrillated cellulose (NFC) matrix. …”

The Mechanical Behavior of Mutant K14-R125P Keratin Bundles and Networks in NEB-1 Keratinocytes by Daniel R. Beriault (184352)

“…Disassembly of the F-actin network with Latrunculin A unexpectedly led to a marked decrease in stretch-induced necrosis in both WT and mutant cells. …”

The effects of Hb binding site number and strength on noise. by David M. Holloway (95831)

“…<p>(A) Simulation of lacZ expression from 6 Bcd sites (3A3X) and one Hb site (in a WT background), compare to (B) lacZ expression in an embryo with the pThb1 promoter construct. …”

Data_Sheet_1_Effects of the probiotic Lactiplantibacillus plantarum IMC 510® on body composition, biochemical parameters, gut microbiota composition and function, and clinical symp... by Giuditta Pagliai (8105576)

Schematic of experimental paradigms. by Shiva Farashahi (5018381)

“…Following the appearance of the fixation cross and information about the maximum reward (50 and 100 points), subjects were presented with a diminishing arc informing them about the rate of reward decrease on a given trial. …”

Preferential down-regulation of miRNAs and overexpression of XY genes in GCKO testis samples by P18. by Anne R. Greenlee (304274)

“…B) Validation of Febit miRNA array fold changes by qRT-PCR and primers amplifying miR-34b-3p, -15b-5p, -19a and -191. In comparison to WT (n  = 3; RFC  = 1.0), significant reductions in GCKO miRNA expression were observed using qRT-PCR and P18 RNA from GCKO testes (n = 3). …”

Targeted deletion of PPARγin macrophages inhibits lung cancer metastasis in an orthotopic mouse model of non-small cell lung cancer. by Howard Li (194066)

“…<p>Following lethal irradiation, WT C57BL/6 mice received bone marrow from either PPARγ-Mac^neg (KO) or PPARγ^flox/flox (Flox) donor mice as described in the “<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028133#s4" target="_blank">Methods</a>” section. …”