Gcn4 dependent outputs can sustain high translation capacity during growth. by Rajalakshmi Srinivasan (395110)

“…Barplots, comparing the relative amount of proteins translated (in a methionine-dependent manner), for the genes shown in Fig 5B. These selected genes were cloned in frame with luciferase in an inducible system, to create a translation-reporter (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1009252#pgen.1009252.s014" target="_blank">S14 Fig</a>), and translation of these were induced in WT or <i>Δgcn4</i> cells in methionine supplemented medium (MM+Met). …”

Hyperacetylation induced by chemical HDAC inhibition stresses the replication fork of FANCA deficient cells. by Benilde García-de Teresa (17838878)

“…<b>(D)</b> Representative immunoblots after capture of the replication fork of SAHA treated wild type and FA cells. Lanes 1 to 5 total protein of INPUT samples: lane 1 no click control, iWT cells in lanes 2 (pulse) and 3 (SAHA and pulse), FA-A cells in lanes 4 (pulse) and 5 (SAHA and pulse). …”

PINK1 expression alters mitochondrial function and cellular viability. by Anna Sandebring (263540)

“…A control lentivirus expressing LacZ is shown in lane 1, lanes 2–4 are cell lines expressing wild-type (WT), G309D or kinase dead (KD) PINK1. The PINK1 in these cells is V5-tagged at the C-terminus, the precursor (arrow) and mature (closed arrowhead) forms of PINK1 are visible. …”

Effect of FAAH inhibition on depressive-like behavior in WKY rats. by K. Yaragudri Vinod (323103)

“…<p>Treatment with URB597 (0.3 mg/kg, i.p. for 7 days) elicited a significant decrease in total time spent in immobility (50%, p<0.01; A) and a marked increase in sucrose intake (48%, p<0.05; B) without any effect on the spontaneous locomotor activity in the open field (C) in WKY rats compared to vehicle treated WKY rats.…”

c-MET Protects Breast Cancer Cells from Apoptosis Induced by Sodium Butyrate by Bo Sun (177052)

Schematic illustration of the variable-ranking process for the derivation cohort. by Johan Nilsson (364438)

Lack of off-target silencing of other keratins by lead inhibitor for K12 Leu132Pro mutation, siRNA K12-L132P-9. by Haihui Liao (192426)

Kinetics of differential gene expression after rapid degradation of endogenous OGT in MEFs. by Sara Formichetti (20536052)

“…Differentially expressed genes with p-value < 10⁻⁵ and absolute log₂FC > 0.3 (i.e. 1.2-fold increase or decrease in expression) are labeled in red and their number is indicated. …”

Data_Sheet_1_Mitofusin 2 Is Essential for IP3-Mediated SR/Mitochondria Metabolic Feedback in Ventricular Myocytes.PDF by Lea K. Seidlmayer (6997946)

“…In contrast to ET-1, ISO induced a decrease in ATP levels in WT myocytes. Mfn2 KO abolished ATP generation upon ET-1 stimulation but increased ATP levels upon ISO application with highest levels observed in PNM regions.…”

Data_Sheet_1_Mitofusin 2 Is Essential for IP3-Mediated SR/Mitochondria Metabolic Feedback in Ventricular Myocytes.PDF by Lea K. Seidlmayer (6997946)

“…In contrast to ET-1, ISO induced a decrease in ATP levels in WT myocytes. Mfn2 KO abolished ATP generation upon ET-1 stimulation but increased ATP levels upon ISO application with highest levels observed in PNM regions.…”

Datasheet1_Heart rate reduction after genetic ablation of L-type Cav1.3 channels induces cardioprotection against ischemia-reperfusion injury.docx by Viviana Delgado-Betancourt (16719402)

“…Consistently, heart rate reduction in Ca_v1.3^+/− or by the f-channel blocker ivabradine was associated with significant decrease in infarct size (−27% and −32%, respectively) in comparison to WT mice.…”

The sequence between nt. 626/640 mediates both TPA-dependent activation and the negative regulation by T3/TR. by Hiroko Misawa (335240)

“…The stimulatory effect by TPA is decreased in MA. In the presence or absence of TPA (100 ng/ml), pBL-FFL-CAT5 (WT) or its mutants (MA-D) were transfected into CV1 cells. …”

Repression of <i>hmsT</i> by Fur. by Fengjun Sun (113206)

“…<b>b) LacZ fusion.</b> A promoter-proximal region of <i>hmsT</i> was cloned into the <i>lacZ</i> transcriptional fusion vector pRW50, and transformed into WT or <i>Δfur</i> to determine the <i>hmsT</i> promoter activity, i.e., the β-Galactosidase activity (Miller units) in the cellular extracts. …”

Soy isoflavones genistein and daidzein protect primary cortical cultures from Tat neurotoxicity. by Sheila M. Adams (322032)

“…<p>Primary cortical neurons were exposed to estrogen (0.1, 2.0 and 10 nM), or isoflavones (0.05, 0.2 and 1 µM) 24 hr prior to the start of Tat_1–86 B (50 nM) treatment. Cell viability was assessed by Live/Dead assay. …”

Table_1_The Metabolic Changes and Immune Profiles in Patients With COVID-19.pdf by Bing He (135034)

“…<p>To explore the metabolic changes and immune profiles in patients with COVID-19, we analyzed the data of patients with mild and severe COVID-19 as well as young children with COVID-19. …”

Changes in expression of epithelial and mesenchymal proteins in primary human small airway epithelial cells (SAEC) and large airway epithelial cells (LAEC) stimulated with TGFβ1 <i... by Balarka Banerjee (107946)

“…(A) Initial immunoblots suggested a marked increase in mesenchymal markers and corresponding decrease in epithelial markers after 96 h of stimulation. …”

Phenotypic analyses of tumour cells in co-culture experiments. by Victor Trevino (8900)

“…The presence of normal cells are seen to (A) increase the population doubling time (PDT), (B) decrease the total cell number, (C) have no significant effect on apoptosis, (D) decrease the number and (E) size of the cell clusters and (F) increase the number of single cells. …”

RPL37, RPS15, and RPS20 bind to and regulate MdmX levels. by Lilyn Daftuar (433919)

“…Endogenous proteins were detected by immunoblotting with the indicated antibodies. (g) Decrease in MdmX mRNA by RPL37. U2OS cells were seeded in 60 mM tissue culture plates and transfected with Myc-RP (0–5.0 µg). …”

Supplementary Material for: Effect of Heme Oxygenase-1 Deficiency on Glomerular Proteomics by Detsika M.G. (3195288)

Table_15_Transcriptome characteristics during cell wall formation of endosperm cellularization and embryo differentiation in Arabidopsis.xlsx by Chengcheng Li (2591632)

“…Here, we characterized the early embryo and endosperm development of the naa15 mutant that had abnormal embryo differentiation and incomplete endosperm cellularization compared to WT of Arabidopsis, and comparatively investigated the changes of gene expressions in WT seeds at 3, 4, and 5 days after pollination (3W, 4W, and 5W) and the white homozygous aborted naa15 seeds at 5, 6, and 7 DAP (5M, 6M, and 7M) from naa15-1/+ siliques using RNA sequencing and qPCR assays. …”